LOCUS       sequence-273658-        5322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Zea mays FNR and Prochlorococcus marinus NATL2A SIR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Campbell IJ, Olmos JL Jr, Xu W, Kahanda D, Atkinson JT, Sparks ON, 
            Miller MD, Phillips GN Jr, Bennett GN, Silberg JJ
  TITLE     Prochlorococcus phage ferredoxin: Structural characterization and 
            electron transfer to cyanobacterial sulfite reductases.
  JOURNAL   J Biol Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501.
  PUBMED    32434930
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501."
COMMENT     Sequence Label: pSAC16
FEATURES             Location/Qualifiers
     source          1..5322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(159..164,165..181,182..188)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_feature    2086..2106
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2161..2188
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        join(2208..2213,2214..2230,2231..2237)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      3312..3383
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3399..3426
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3587..4378
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      4390..4484
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4598..5143
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     5044..5063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5260..5277
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtctgcaaag tctcgaaata ttaaaccaga gaataaataa ttaaacgcca aaatatttct
       61 agcaaaaacc ccagttatta aaccgcctaa gtcccccagg aaagggggat ataacagtat
      121 agattttgtc agccttcagc ttggctttac cgtcagagtt gacaattaat catccggctc
      181 gtataatgtg tggagggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt
      241 cgtactgaaa catcttaatc atgcaatgga ggctttctaa tgaacttcga cgaaaaagaa
      301 tcaacaaaca tcgaagaaat cgaacagacg gtcccatccc catgcattgc caacaattcc
      361 ccacgcgcaa agttcgaaca atttaaggca gatagtaatt atctgtcaga gccgttgcac
      421 agtgagttgc ttaatgactc ggaccatttt acgaatgatg cggtacaact gctgaaattc
      481 cacggttcat accaacagga cgaccgtgaa catcgcaaac gtggcggtac agggaaagac
      541 tggcaaatga tgttgcgcct gcggaatcca gctgggtatg tacctggccc gctcttcgtg
      601 gccttagatg agctttcgga tcggcttggc aatcagactt tacgtgctac cactcgtcaa
      661 tgcttccaga tgcatgggat caaaaagggc aacattaagg aagtgatcgg gacgattgta
      721 aaaagcatgg gctcgactct ggcagcctgt ggggatgtta atcggaacgt gatggcaccg
      781 gccgcaccat acgagcaagg ttcgtatcct gctgcacgta aattggctaa cgatatcgct
      841 gacgttcttt ctccgcagaa ggccgaaaaa acgtatatcg atctctgggt agatggtgaa
      901 atgaagtatg ccattaaacc ttcaagcgag gtgaagaaga acaaaaagct ccaattgaaa
      961 ccgggggtat ttagtgggga caaaaaggag ccgttgtacg gtgcaacata tttaccacgg
     1021 aaattcaaat gtgccactac cgtgccaggg gacaattcag tggatattct gacacatgat
     1081 attggtctgg tgactttcac caataaaaag ggggttttgg aaggctgcaa cgtgtacgta
     1141 ggtggtggga tgggccgcac gcacaattta gacacgacgt ttgcccggat cgcggaccct
     1201 attgggtatg tagagggtga gcacattctg gagcttgtgc agtccatcct tgcgctgcaa
     1261 cgcgactatg gtgatcgtaa gacccggcgc cattcgcgcc tgaagtacgt tctccatgat
     1321 atgggggtcg attggttcaa aaaacagctc acaactaaat atttcacacg tcaaatcgag
     1381 aacctcaagc acgagggcga caccatcctg gaagattatc tcggctggca ccagcagtcg
     1441 gaaaagctct ggtttgtcgg ccttccactc ttgtccgggc gtctcactgg ccgggttaag
     1501 aaagagctcc gcaatatcgt cgaaaaattt gctttagatg tccgcctgac cccaaatcag
     1561 gacctcttac tttgtaacat tggtaactac cagaaagctt ccgtgaaacg ggccctgatt
     1621 aatatcgggt ttatcaaccc aggcgcccct gactccttag cgcggaatgc catggcctgt
     1681 ccagcgttac cactttgtgg cctcgcaatg accgaggccg agcgctttct cccggagctg
     1741 ttggaacgca ttaataacca gctcaagatc cttgaaatta acaagcctat cttaattcgc
     1801 gtgactggtt gtccaaatgg ctgtgcgcgg ccatatatgg ccgaattagc gttggttggt
     1861 agtgggctca accaatatca gctttggctg ggtggctcta caaacctcaa gcgtctcgcg
     1921 accccttatt tgcagaagat gcctattgat gacttggaaa aaacactgga acctcttttt
     1981 ctcagttgga aagatacggg tgcgagtagt tcactcggtg atcatgtcac taaattaggc
     2041 tcagagtctg taatgtcgct tctcacctcc tctgcagccc cgtgatacta gtagcggccg
     2101 ctgcaggcat gcctcgagat gcatggcgcc taacctaaac tgacaggcat caaattaagc
     2161 agaaggccat cctgacggat ggcctttttg cgtttcgaac aattgaattg acaattaatc
     2221 atccggctcg tataatgtgt ggagggccca agttcactta aaaaggagat caacaatgaa
     2281 agcaattttc gtactgaaac atcttaatca tgcaatggag gctttctagg agagcattag
     2341 gtctcgatgg gcagtgttca acaggcttct aggtcaaaag tatccgtcgc accattgcat
     2401 ttagaaagtg ccaaggagcc tccactaaat acttataaac ctaaggaacc atttacagct
     2461 accattgtga gcgttgagtc tcttgtaggt cctaaagcac caggagaaac ttgtcacata
     2521 gtcatcgatc atggcggtaa cgtgccttac tgggaaggac aatcatatgg cgttattcca
     2581 cctggtgaga atccaaagaa acctggtgcc ccacagaacg taaggttgta ctccatagct
     2641 agtacaagat atggagacaa tttcgatggc aggaccggta gcttatgcgt cagaagagca
     2701 gtttactatg accctgaaac tggaaaggaa gatccatcta aaaacggtgt gtgttcaaat
     2761 tttctatgca actccaagcc aggtgacaaa atccaactta caggtcctag tggaaagatt
     2821 atgttgttac cagaggaaga tcctaatgca acccacataa tgatcgctac tggtacaggc
     2881 gttgcaccat tcaggggtta cttgagaaga atgtttatgg aagatgtacc taactatagg
     2941 ttcggaggtt tagcttggct atttcttggc gtcgccaatt ctgacagctt gttatacgat
     3001 gaggagttca cctcatatct aaaacaatac ccagacaatt ttagatatga taaggcattg
     3061 tccagggagc agaaaaacag aagtggaggt aaaatgtacg tgcaagataa gattgaagaa
     3121 tattctgacg agattttcaa attacttgat ggcggtgctc atatatactt ttgtggattg
     3181 aagggtatga tgcctggcat ccaagacact ttaaaaaagg ttgccgaaag aaggggtgaa
     3241 tcatgggatc agaaactagc acaacttaag aaaaataagc agtggcacgt agaggtctat
     3301 taaccaggca tcaaataaaa ctaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3361 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     3421 tctgcgttta taggtaccag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     3481 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     3541 caggggatca agatctgatc aagagacagg atgaggatcg tttcgtatga gggaagcggt
     3601 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     3661 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     3721 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     3781 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     3841 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     3901 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     3961 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     4021 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     4081 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     4141 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     4201 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     4261 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     4321 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataaga
     4381 gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     4441 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc actagtaaca
     4501 acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat tgcactgaaa
     4561 tctagtaata ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg
     4621 taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct
     4681 gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg
     4741 tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta
     4801 ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag
     4861 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
     4921 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa
     4981 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc
     5041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
     5101 tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc
     5161 cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg
     5221 taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa
     5281 gcggaatata tcccctaggt ggagtctggc ctcaaataca aa
//
