LOCUS       sequence-273664-        3912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Prochlorococcus marinus NATL1A  Fd 1 fused to mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Campbell IJ, Olmos JL Jr, Xu W, Kahanda D, Atkinson JT, Sparks ON, 
            Miller MD, Phillips GN Jr, Bennett GN, Silberg JJ
  TITLE     Prochlorococcus phage ferredoxin: Structural characterization and 
            electron transfer to cyanobacterial sulfite reductases.
  JOURNAL   J Biol Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501.
  PUBMED    32434930
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501."
COMMENT     Sequence Label: pFd029.R2.RFP
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1032
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     terminator      1062..1133
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1149..1176
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1228..1262
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             1297..1923
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1373..1392)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      1929..2015
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2180..2768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2260..2279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2856..2950)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2971..3630)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3545..3564
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3799..3817
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(3838..3856,3857..3862,3863..3879,3880..3885,
                     3886..3911)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    3838..3856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3863..3881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 attaccggca acaggagaca caaaatgacc tccttcaaaa tcaccttcat caacgaattg
       61 agcggtctga aagaaattat tgatatccct gatgataaat acattctgga cgccgcatgc
      121 gaacaaaaca tcgaactgcc tgcatcctgc cgggaaggca cttgctcgac ctgcgtggca
      181 aagttggaaa aagggaacgt agaccaacag gaccagagct tcttggatga cgaccagatt
      241 gaaaagggtt acattctgat ctgtaaagcg tacccactgt cagattgtat tattcggact
      301 catgcggaag accagttata tgggggctct ggaggttctg gaggttctgg tgggtccgcg
      361 agtagcgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt
      421 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag
      481 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc
      541 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac
      601 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt
      661 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt
      721 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt
      781 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
      841 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
      901 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
      961 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
     1021 accggtgctt aagaattctg ataataatct agaccaggca tcaaataaaa cgaaaggctc
     1081 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1141 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggtacccg tggatcctct
     1201 agagtcgacc tgcaggcatg caagcttttt acggctagct cagtcctagg tatagtgcta
     1261 gcccagccag agaaacactc tttaacaggg ggtagtatga tgtctcgttt agataaaagt
     1321 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
     1381 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
     1441 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     1501 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     1561 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     1621 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     1681 gagaatgcat tatatgcact cagcgcagtg gggcatttta ctttaggttg cgtattggaa
     1741 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     1801 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     1861 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtct
     1921 taaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1981 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagacc tagggcgttc
     2041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2101 gggataacgc atgcaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     2641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2821 cacgttaagg gattttggtc atgactagtg cttggattct caccaataaa aaacgcccgg
     2881 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2941 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     3001 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     3061 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     3121 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     3181 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     3241 gttttcacca taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     3301 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     3361 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     3421 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     3481 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     3541 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     3601 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     3661 taactcaaaa aatacgaccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     3721 tacgggccgg ccaacgtctc attttcgcca gatatcgacg tctaagaaac cattattatc
     3781 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagtcc
     3841 ctatcagtga tagagattga catccctatc agtgatagag atactgagca catcagcagg
     3901 acgcactgac cg
//
