LOCUS       sequence-273665-        5313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Zea mays FNR and Prochlorococcus marinus MIT9211 SIR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Campbell IJ, Olmos JL Jr, Xu W, Kahanda D, Atkinson JT, Sparks ON, 
            Miller MD, Phillips GN Jr, Bennett GN, Silberg JJ
  TITLE     Prochlorococcus phage ferredoxin: Structural characterization and 
            electron transfer to cyanobacterial sulfite reductases.
  JOURNAL   J Biol Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501.
  PUBMED    32434930
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501."
COMMENT     Sequence Label: pSAC17
FEATURES             Location/Qualifiers
     source          1..5313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     143..160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(364..369,370..386,387..393)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_feature    2282..2302
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2357..2384
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        join(2404..2409,2410..2426,2427..2433)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      3508..3579
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3595..3622
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3783..4574
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      4586..4680
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(4794..5313,1..26)
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     5240..5259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt
       61 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg
      121 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatccccta
      181 ggtggagtct ggcctcaaat acaaagtctg caaagtctcg aaatattaaa ccagagaata
      241 aataattaaa cgccaaaata tttctagcaa aaaccccagt tattaaaccg cctaagtccc
      301 ccaggaaagg gggatataac agtatagatt ttgtcagcct tcagcttggc tttaccgtca
      361 gagttgacaa ttaatcatcc ggctcgtata atgtgtggag ggcccaagtt cacttaaaaa
      421 ggagatcaac aatgaaagca attttcgtac tgaaacatct taatcatgca atggaggctt
      481 tctaatgact aaggaagcct tgtctttgcc ggacatcgat gactcgccat tgtccccgtg
      541 cgttgcaaat gggcaagatc gtacaaagtt tgagcaattc aaagcagaca gcgattacct
      601 gaaggaaccg cttcggacgg aattgcagaa caattcggat cacttctcaa atgacgcggt
      661 acaattgctt aagtttcatg gcagctatca acaggacaac cgcgacacgc gccagaaagg
      721 tgtggaaaag gattggcaaa tgatgttacg gttacgttcg ccgggcggcc atattccacc
      781 agaactgttt gtcgcgttag atcatctgag tgaccaatta ggtaacggta ctcttcgcgc
      841 tactacgcgg caggcgtttc aaatgcacgg tattaagaag gagtcgctga aaaccgtaat
      901 tgaaactatc attcgctcta tgggttctac tttagcggcg tgtggggaca ttaaccgtaa
      961 tgtaatggcg ccagcggcac cgttcacgta tggtgattat ccagtcgctc ggcggctggc
     1021 aaaccagatt gcggacgtgc tttcaccttc ttacgcggag aagacttatt tagagctttg
     1081 ggttgatggc gatattagct ataagatttc accttctaac gaggtgaaga aggtgcggaa
     1141 aaaccagtac aaggagggtg tctattcagg tgacatcaag gagccgctct acggctcaac
     1201 ttacttgcct cggaagttta aatgcgcagt aactgttccg ggggacaata gtgtcgatct
     1261 cttaacacaa gacattgggc ttgttgtttt caccaacgcg aaaaataagt taattggctg
     1321 caatgtttac gtggggggcg ggatggggcg cacccataac aatgaagaga catttgcccg
     1381 gagtgctgag ccgatcgggt ttgtgagtgc ggagtatgtt ctggaactgg ttcagtctat
     1441 tctggcttta cagcgtgact acggggaccg taaggttcgg cgccatgccc gtatgaaata
     1501 ccttatcaat gacaagggga tcgagtggtt catcgataag ttgaagaaca attacttcaa
     1561 atatccaatt aaaagtttgc gtaaagaacc gacttccaaa ctcttggatt atctcggttg
     1621 gcataaacag tcacacgatc tttattttgt tggcattcct ctgcttagtg gtcgtttgag
     1681 tggcgaatac aagaaagcta tttgtaagtt agtgaacaaa tttaagcttg atattcagct
     1741 gacgccgaac caagatttac ttctgtgtaa catcgggagt tatcagcgtt cttctattaa
     1801 aaaagaatta agcaatattg ggatcattaa gccggatagt ccggaaccga tccagcgtca
     1861 cgctctggct tgccctgcct tgccgctttg cggcctcgca gtaacagaag cagagcgcat
     1921 cttaccggag atcttagatc gtattaacac ccaattatca gacttgcgca ttcagaaaac
     1981 acttcttttc cgcatgactg ggtgtccaaa cggttgtgca cggccataca tggcggagtt
     2041 agctttggtg gggtcggggc ttgatcaata ccagttgtgg ttagggggct ctcctaatct
     2101 gcaacggctc gcgaagcctt tcattcagcg gatgcctctc actagcctcg aggagacact
     2161 taagccactg ttcattagct ggaaaaattc caagaaggag atcagcttcg gtgaccatat
     2221 gaacgagctg ggggaccaga acatcatgga acttctttca ttgaaagaca aggagccttg
     2281 atactagtag cggccgctgc aggcatgcct cgagatgcat ggcgcctaac ctaaactgac
     2341 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tcgaacaatt
     2401 gaattgacaa ttaatcatcc ggctcgtata atgtgtggag ggcccaagtt cacttaaaaa
     2461 ggagatcaac aatgaaagca attttcgtac tgaaacatct taatcatgca atggaggctt
     2521 tctaggagag cattaggtct cgatgggcag tgttcaacag gcttctaggt caaaagtatc
     2581 cgtcgcacca ttgcatttag aaagtgccaa ggagcctcca ctaaatactt ataaacctaa
     2641 ggaaccattt acagctacca ttgtgagcgt tgagtctctt gtaggtccta aagcaccagg
     2701 agaaacttgt cacatagtca tcgatcatgg cggtaacgtg ccttactggg aaggacaatc
     2761 atatggcgtt attccacctg gtgagaatcc aaagaaacct ggtgccccac agaacgtaag
     2821 gttgtactcc atagctagta caagatatgg agacaatttc gatggcagga ccggtagctt
     2881 atgcgtcaga agagcagttt actatgaccc tgaaactgga aaggaagatc catctaaaaa
     2941 cggtgtgtgt tcaaattttc tatgcaactc caagccaggt gacaaaatcc aacttacagg
     3001 tcctagtgga aagattatgt tgttaccaga ggaagatcct aatgcaaccc acataatgat
     3061 cgctactggt acaggcgttg caccattcag gggttacttg agaagaatgt ttatggaaga
     3121 tgtacctaac tataggttcg gaggtttagc ttggctattt cttggcgtcg ccaattctga
     3181 cagcttgtta tacgatgagg agttcacctc atatctaaaa caatacccag acaattttag
     3241 atatgataag gcattgtcca gggagcagaa aaacagaagt ggaggtaaaa tgtacgtgca
     3301 agataagatt gaagaatatt ctgacgagat tttcaaatta cttgatggcg gtgctcatat
     3361 atacttttgt ggattgaagg gtatgatgcc tggcatccaa gacactttaa aaaaggttgc
     3421 cgaaagaagg ggtgaatcat gggatcagaa actagcacaa cttaagaaaa ataagcagtg
     3481 gcacgtagag gtctattaac caggcatcaa ataaaacgaa atgctcagtc gaaagactgg
     3541 gcctttcgtt ttatctgttg tttgttggtg aacgctctct actagagtca cactggctca
     3601 ccttcgggtg ggcctttctg cgtttatagg taccagcaag cgaaccggaa ttgccagctg
     3661 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     3721 caaggatctg atggcgcagg gtatcaagat ctgatcaaga gacaggatga ggatcgtttc
     3781 gtatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     3841 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     3901 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     3961 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     4021 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     4081 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     4141 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     4201 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     4261 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     4321 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     4381 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     4441 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     4501 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     4561 tagtcggcaa ataagagctc gcttggactc ctgttgatag atccagtaat gacctcagaa
     4621 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat
     4681 ccaagcacta gtaacaactt atatcgtatg gggctgactt caggtgctac atttgaagag
     4741 ataaattgca ctgaaatcta gtaatatttt atctgattaa taagatgatc ttcttgagat
     4801 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt
     4861 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg
     4921 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
     4981 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
     5041 ggactcaaga cgatagttac cggataaggc gcagcggtag gactgaacgg ggggttcgtg
     5101 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     5161 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     5221 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5281 accactgatt tgagcgtcag atttcgtgat gct
//
