LOCUS       sequence-273673-        8019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spizellomyces Hsp70 promoter driving expression of Hygromycin 
            resistance fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C,
            Fritz-Laylin LK, Buchler NE
  TITLE     Genetic transformation of Spizellomyces punctatus, a resource for 
            studying chytrid biology and evolutionary cell biology.
  JOURNAL   Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.
  PUBMED    32392127
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-11"; volume: "9. pii: 52741. doi"; pages: " 
            10.7554/eLife.52741"
COMMENT     Sequence Label: pEM09
FEATURES             Location/Qualifiers
     source          1..8019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      746..1334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1235..1254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1488..1505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(1917..1922,1923..1940,1941..1947)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1955..1971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1960..1982
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1979..1995
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1979..1995
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2014..2034
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2016..2034
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3135..4160
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             3135..4160
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             4179..4889
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4206..4234)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4829..4849
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      4912..5099
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4967..4991)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(5549..5568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5550..5568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5578..5595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5578..5594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5587..5609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5735..6190
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5822..5841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6032..6053
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6321..7124)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7125..7340)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7364..7383)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7483..7505
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7543..7561)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7629..7733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7734..7802,7803..8019,1..575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7952..7971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
       61 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
      121 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
      181 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
      241 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
      301 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
      361 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
      421 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
      481 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
      541 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
      601 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
      661 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
      721 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      781 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      841 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      901 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      961 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1021 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1081 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1141 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1201 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1261 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1321 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1381 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1441 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1501 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1561 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     1621 ttaatgtgag ttacctcact cattaggccg ttgatatagg ccagtaacta atgcagctac
     1681 tgcatagaat cgatcaagag actcgagttt cggcaatgga actgtgcgcg gagcatctcc
     1741 aagtgtggat ttcgtagaga aggttgtgac tcctgccatt gcaaaagtag caaaaggctc
     1801 ccagggatct tctctcacct cattgaaaaa agcctttgaa cttgcggaac aggtatgttg
     1861 acaactgtac gcatagacaa catgagtcgg tctaatgcat actttgacac ctttcattta
     1921 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     1981 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     2041 aagctggagc tgagctcttt taaaatcttg tctttgtgca ctcttctctc cctgtcatat
     2101 tgctttacgt cgagagactg gtcaaaggat ccggtgagat cattgagcgc attgttaccc
     2161 aaaatcagca tcgagaaatt aacaagatcg cgacgctggg cgacgggaac atgtatgcga
     2221 gcacattgga taaggttgcc aaaggcggtc gatagagctc tagtatttga atagtgattc
     2281 ctgctgctca ttctcacaaa tgtctatgaa tgtcgtctgt ccatcgcttg aaatcaaaga
     2341 atgcgataca tggcgtgtcc tgatgcagcg attccaattg taaatgcgtg catgtgttcc
     2401 atgggcctgc gttaaaatgg attctgcatg tgcagcaata aatcaataaa aggatgagac
     2461 atgcagcaag gaattcactg aaagctatgg aaggatatcg aataaagaac aatttggaag
     2521 ctcatagaaa ttggatgtga tggtccaaaa tggatttccg ttttgctttc cgcgcgacgc
     2581 tctttttctt tttacttgtc ttctttcaac aaaccccccc ttcttctact ttctcgttga
     2641 aaacgataca aggaagaaag cgagagttca aaaggtaata aaagcccttg cttttgcagt
     2701 ttgcttttac gtagagtttg ttcgcgtcat cagccagagc gcccttttac ctggcaacgc
     2761 gacgctttca atttatttct cgctgtgtga aatacttttc atgctgacta tcccttgtcc
     2821 atccccattc cttccgtcat ttgcttgcta gctcgctcgc gaagattccc tctcaatcct
     2881 tctccctatt tggcaccctc ctcccgacac cgggcactaa cctcgccgca gatattctca
     2941 tcggcgtcgt cgctattgga gttgtaggcc tacttgcaaa cttcctcttt cgcagaacat
     3001 caagcagcac caagcgtcgg cgtaataaaa aacgcacgaa gcaagaagct actcatcgtc
     3061 aacaggagcc gcttttagac gagcgacata aagacactac cagtaacgga ccaaagatca
     3121 gcacaattta attaatgggt aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     3181 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     3241 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     3301 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     3361 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     3421 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     3481 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     3541 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     3601 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     3661 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     3721 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     3781 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     3841 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     3901 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     3961 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     4021 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     4081 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     4141 ctcgtccgag ggcaaaggaa ggaggaggag gaggaggaag taaaggagaa gaacttttca
     4201 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     4261 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     4321 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcact tatggtgttc
     4381 aatgcttttc aagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc
     4441 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     4501 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     4561 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     4621 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     4681 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     4741 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     4801 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     4861 ttacacatgg catggatgaa ctatacaaat aggcgcgcca cttctaaata agcgaatttc
     4921 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt
     4981 taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag
     5041 gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc
     5101 agatccgcta gggataacag ggtaatattg tttagcttgc ctcgtccccg ccgggtcacc
     5161 cggccagcga caggaacacc cagggatagt gcagcaattg ttggtggaaa tgatggacaa
     5221 attggaaata cgataagtgg ggattcattt tgggaaaaga caaaagaaag atgctttagg
     5281 gacctatggg gggtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
     5341 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
     5401 aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg
     5461 tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg
     5521 ctttaatttg cggccggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     5581 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5641 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5701 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     5761 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5821 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5881 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5941 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6001 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6061 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6121 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6181 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     6241 tagggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag tatacatgca
     6301 tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata tgcttcccag
     6361 cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc aaatagtcct
     6421 cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt tctatactgt
     6481 tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat caaccaatcg
     6541 taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac aaaatctttg
     6601 tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag ggagcccttg
     6661 catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc ttctgccgcc
     6721 tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt aatgtctgcc
     6781 cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt accaatgtca
     6841 gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc
     6901 ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt tagtaaacaa
     6961 attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg aacatccaat
     7021 gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc agcaacagga
     7081 ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta tcttcgtttc
     7141 ctgcaggttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca
     7201 acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc
     7261 tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa
     7321 aaaaaaaatg atgaattgaa aaggtggtat ggtgcactct cagtacaatc tgctctgatg
     7381 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7441 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7501 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7561 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7981 acgccgggca agagcaactc ggtcgccgca tacactatt
//
