LOCUS       sequence-273688-       12420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium  T-DNA  with SpunHsp70pr driving hph-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C,
            Fritz-Laylin LK, Buchler NE
  TITLE     Genetic transformation of Spizellomyces punctatus, a resource for 
            studying chytrid biology and evolutionary cell biology.
  JOURNAL   Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.
  PUBMED    32392127
REFERENCE   2  (bases 1 to 12420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-11"; volume: "9. pii: 52741. doi"; pages: " 
            10.7554/eLife.52741"
COMMENT     Sequence Label: pGI3EM09
FEATURES             Location/Qualifiers
     source          1..12420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..630)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     rep_origin      1791..3133
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2754..2801)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3174..3192
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3395..3610
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3611..4414
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    4581..4605
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4786..4808
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4800..4817
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4801..4817
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5904..6929
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             5904..6929
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             6948..7658
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6975..7003)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7598..7618
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      7681..7868
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7736..7760)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7883..7899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7883..7899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7896..7918)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7907..7923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7931..7937,7938..7955,7956..7961))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7976..7997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8174..8198
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(8480..8499)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8491..9306
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             8491..9306
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8564..8583)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      9401..9989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9890..9909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10143..10160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10175..10315
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      10659..10853
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10919..11992)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
       61 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
      121 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
      181 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
      241 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
      301 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
      361 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
      421 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
      481 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
      541 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
      601 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
      661 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
      721 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
      781 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
      841 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
      901 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
      961 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
     1021 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
     1081 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
     1141 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
     1201 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
     1261 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
     1321 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
     1381 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
     1441 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
     1501 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
     1561 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
     1621 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
     1681 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
     1741 cgttttcaga agacggctgc actgaacgtc agaaggaaaa gtgccacctg aacgaagcat
     1801 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     1861 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
     1921 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     1981 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca
     2041 acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc
     2101 taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg
     2161 ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt
     2221 ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc
     2281 gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg
     2341 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga
     2401 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt
     2461 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact
     2521 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt
     2581 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag
     2641 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt
     2701 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc
     2761 ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga
     2821 gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta
     2881 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg
     2941 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct
     3001 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct
     3061 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct
     3121 ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3181 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     3241 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     3301 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     3361 agcagattgt actgagagtg caccatacca ccttttcaat tcatcatttt ttttttattc
     3421 ttttttttga tttcggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga
     3481 agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga
     3541 aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct gcaggaaacg
     3601 aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt
     3661 tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga
     3721 tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt
     3781 actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct
     3841 aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat
     3901 tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga
     3961 cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc
     4021 agaagaagta acaaaggaac ctagaggcct tttgatgcta gcagaattgt catgcaaggg
     4081 ctccctatct actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga
     4141 ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg
     4201 gttgattatg acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta
     4261 tagaaccgtg gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact
     4321 atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga
     4381 agcatatttg agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg
     4441 tatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac ctggcatgca
     4501 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
     4561 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
     4621 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tcattcgcca ttcaggctgc
     4681 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     4741 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     4801 gtaaaacgac ggccagtgcc aagctttttt aaaatcttgt ctttgtgcac tcttctctcc
     4861 ctgtcatatt gctttacgtc gagagactgg tcaaaggatc cggtgagatc attgagcgca
     4921 ttgttaccca aaatcagcat cgagaaatta acaagatcgc gacgctgggc gacgggaaca
     4981 tgtatgcgag cacattggat aaggttgcca aaggcggtcg atagagctct agtatttgaa
     5041 tagtgattcc tgctgctcat tctcacaaat gtctatgaat gtcgtctgtc catcgcttga
     5101 aatcaaagaa tgcgatacat ggcgtgtcct gatgcagcga ttccaattgt aaatgcgtgc
     5161 atgtgttcca tgggcctgcg ttaaaatgga ttctgcatgt gcagcaataa atcaataaaa
     5221 ggatgagaca tgcagcaagg aattcactga aagctatgga aggatatcga ataaagaaca
     5281 atttggaagc tcatagaaat tggatgtgat ggtccaaaat ggatttccgt tttgctttcc
     5341 gcgcgacgct ctttttcttt ttacttgtct tctttcaaca aaccccccct tcttctactt
     5401 tctcgttgaa aacgatacaa ggaagaaagc gagagttcaa aaggtaataa aagcccttgc
     5461 ttttgcagtt tgcttttacg tagagtttgt tcgcgtcatc agccagagcg cccttttacc
     5521 tggcaacgcg acgctttcaa tttatttctc gctgtgtgaa atacttttca tgctgactat
     5581 cccttgtcca tccccattcc ttccgtcatt tgcttgctag ctcgctcgcg aagattccct
     5641 ctcaatcctt ctccctattt ggcaccctcc tcccgacacc gggcactaac ctcgccgcag
     5701 atattctcat cggcgtcgtc gctattggag ttgtaggcct acttgcaaac ttcctctttc
     5761 gcagaacatc aagcagcacc aagcgtcggc gtaataaaaa acgcacgaag caagaagcta
     5821 ctcatcgtca acaggagccg cttttagacg agcgacataa agacactacc agtaacggac
     5881 caaagatcag cacaatttaa ttaatgggta aaaagcctga actcaccgcg acgtctgtcg
     5941 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     6001 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     6061 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     6121 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     6181 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     6241 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     6301 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     6361 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     6421 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     6481 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     6541 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     6601 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     6661 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     6721 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     6781 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     6841 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     6901 gccccagcac tcgtccgagg gcaaaggaag gaggaggagg aggaggaagt aaaggagaag
     6961 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     7021 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
     7081 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcactt
     7141 atggtgttca atgcttttca agatacccag atcatatgaa acggcatgac tttttcaaga
     7201 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
     7261 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     7321 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     7381 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
     7441 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     7501 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     7561 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     7621 ctgctgggat tacacatggc atggatgaac tatacaaata ggcgcgccac ttctaaataa
     7681 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
     7741 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
     7801 ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc
     7861 tctaccggga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7921 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7981 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8041 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct
     8101 agagcaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
     8161 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
     8221 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
     8281 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
     8341 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
     8401 attgtaacga tgacagagcg ttgctgcctg tgatccagat catgaacaat aaaactgtct
     8461 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
     8521 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     8581 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
     8641 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     8701 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     8761 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
     8821 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     8881 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     8941 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     9001 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     9061 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     9121 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     9181 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     9241 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     9301 ttctaagaat taattcatga tcctgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9541 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10141 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
    10201 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
    10261 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
    10321 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
    10381 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
    10441 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
    10501 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
    10561 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
    10621 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
    10681 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
    10741 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
    10801 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
    10861 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
    10921 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
    10981 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
    11041 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
    11101 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
    11161 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
    11221 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
    11281 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
    11341 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
    11401 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
    11461 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
    11521 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
    11581 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
    11641 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
    11701 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
    11761 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
    11821 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
    11881 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
    11941 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
    12001 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc
    12061 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
    12121 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
    12181 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
    12241 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
    12301 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
    12361 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
//
