LOCUS       sequence-273689-       11632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium T-DNA with  SpunH2Bpr driving hph-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C,
            Fritz-Laylin LK, Buchler NE
  TITLE     Genetic transformation of Spizellomyces punctatus, a resource for 
            studying chytrid biology and evolutionary cell biology.
  JOURNAL   Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.
  PUBMED    32392127
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-11"; volume: "9. pii: 52741. doi"; pages: " 
            10.7554/eLife.52741"
COMMENT     Sequence Label: pGI3EM11
FEATURES             Location/Qualifiers
     source          1..11632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..1074)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(1503..2132)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     rep_origin      3293..4635
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(4256..4303)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     4676..4694
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4897..5112
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5113..5916
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    6083..6107
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6288..6310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6302..6319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6303..6319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6618..7643
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             6618..7643
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             7662..8372
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(7689..7717)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8312..8332
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      8395..8582
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8450..8474)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(8597..8613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8597..8613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8610..8632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8621..8637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8645..8651,8652..8669,8670..8675))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8690..8711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8888..8912
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(9194..9213)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9205..10020
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             9205..10020
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9278..9297)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10115..10703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10604..10623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10857..10874
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10889..11029
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      11373..11567
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
       61 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
      121 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
      181 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
      241 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
      301 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
      361 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
      421 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
      481 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
      541 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
      601 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
      661 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
      721 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
      781 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
      841 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
      901 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
      961 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     1021 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
     1081 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
     1141 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
     1201 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
     1261 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
     1321 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
     1381 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
     1441 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
     1501 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
     1561 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
     1621 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
     1681 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
     1741 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
     1801 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
     1861 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
     1921 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
     1981 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
     2041 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
     2101 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
     2161 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
     2221 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
     2281 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
     2341 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
     2401 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
     2461 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
     2521 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
     2581 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
     2641 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
     2701 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
     2761 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
     2821 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
     2881 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
     2941 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
     3001 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
     3061 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
     3121 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
     3181 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
     3241 gtcgttttca gaagacggct gcactgaacg tcagaaggaa aagtgccacc tgaacgaagc
     3301 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     3361 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
     3421 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa
     3481 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
     3541 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
     3601 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
     3661 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
     3721 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
     3781 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
     3841 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
     3901 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
     3961 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
     4021 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
     4081 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
     4141 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
     4201 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
     4261 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac
     4321 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc
     4381 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta
     4441 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
     4501 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag
     4561 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc
     4621 ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag
     4681 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     4741 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     4801 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     4861 agagcagatt gtactgagag tgcaccatac caccttttca attcatcatt ttttttttat
     4921 tctttttttt gatttcggtt tccttgaaat ttttttgatt cggtaatctc cgaacagaag
     4981 gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa
     5041 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa
     5101 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct
     5161 gttgctgcca agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg
     5221 gatgttcgta ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt
     5281 ttactaaaaa cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg
     5341 ctaaaggcat tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac
     5401 attggtaata cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca
     5461 gacattacga atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg
     5521 gcagaagaag taacaaagga acctagaggc cttttgatgc tagcagaatt gtcatgcaag
     5581 ggctccctat ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa
     5641 gattttgtta tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat
     5701 tggttgatta tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag
     5761 tatagaaccg tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga
     5821 ctatttgcaa agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg
     5881 gaagcatatt tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca
     5941 tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt acctggcatg
     6001 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
     6061 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
     6121 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctcattcgc cattcaggct
     6181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6301 ttgtaaaacg acggccagtg ccaagctttt atgctccaag cggagacgat gaggccgata
     6361 ttacagtggc ttagtagcgt gtacaatatt tccataaaag gctacatggt gagtttttca
     6421 cgtgaccgta attagtccca attaggcttt attgtatatt gctactaagc atcgttaaat
     6481 gacctgctcg aagcttcgtc tataaaaggc gggcgtgtgt gcgcttcccc cgctacgtct
     6541 ttttcacaaa cactttgcga cttgaccaag acctttcacc cgcaccactc tcatccatca
     6601 cacacacaaa ttaattaatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     6661 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     6721 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     6781 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     6841 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     6901 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     6961 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     7021 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     7081 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     7141 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     7201 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     7261 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     7321 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     7381 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     7441 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     7501 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     7561 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     7621 gcactcgtcc gagggcaaag gaaggaggag gaggaggagg aagtaaagga gaagaacttt
     7681 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     7741 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     7801 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc acttatggtg
     7861 ttcaatgctt ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca
     7921 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     7981 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     8041 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     8101 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     8161 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     8221 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     8281 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     8341 ggattacaca tggcatggat gaactataca aataggcgcg ccacttctaa ataagcgaat
     8401 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
     8461 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg
     8521 taggtcaggt tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc
     8581 gggaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8641 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8701 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca
     8821 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     8881 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     8941 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     9001 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     9061 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     9121 acgatgacag agcgttgctg cctgtgatcc agatcatgaa caataaaact gtctgcttac
     9181 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctctagg
     9241 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     9301 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
     9361 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     9421 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     9481 gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa
     9541 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     9601 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     9661 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     9721 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     9781 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     9841 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     9901 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     9961 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
    10021 gaattaattc atgatcctgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10081 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10141 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10201 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10261 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10321 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10381 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10441 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10501 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10561 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10621 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10681 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10741 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10801 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10861 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
    10921 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10981 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
    11041 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    11101 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    11161 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
    11221 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
    11281 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
    11341 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
    11401 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
    11461 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
    11521 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
    11581 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta ca
//
