LOCUS       sequence-27373-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Makkinje A, Quinn DA, Chen A, Cadilla CL, Force T, Bonventre JV, 
            Kyriakis JM
  TITLE     Gene 33/Mig-6, a transcriptionally inducible adapter protein that 
            binds GTP-Cdc42 and activates SAPK/JNK. A potential marker 
            transcript for chronic pathologic conditions, such as diabetic 
            nephropathy. Possible role in the response to persistent stress.
  JOURNAL   J Biol Chem. 2000 Jun 9. 275(23):17838-47.
  PUBMED    10749885
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Jun 9. 275(23):17838-47."
COMMENT     Sequence Label: pCMV5-FLAG-Gene33
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             49..72
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagtcaatag cagagctcgt ttagtgaacc gtcagaattg ccaccatgga ctacaaggac
       61 gacgatgaca agaattctat gtcaacagca ggacttgctg ctcaggatat tcgagtccca
      121 ttaaaaactg gatttctcca taatggccag gctttgggga atatgaagac ctgctggggc
      181 agtcgcaatg agtttgaaaa gaatttttta aatatcgatc caataaccat ggcctacaat
      241 ctgaactccc ctgctccgga gcatctaaca accctcggat gtgcttctcc gtccgctcca
      301 gggagcggtc acttctttgc agagcgtggt ccatctccaa agtcaagctt gccccctctt
      361 gttatcccac caagtgaaag ctcgggacag cgtgaagagg atcaagttct gtgtggtttt
      421 aagaaactct cagtgaatgg ggtgtgcgct tccacacctc cacttacacc cattcaaagc
      481 tgctcttccc cgtttccctg tgcagctccc tgtgatcgca gttcccgacc gctcccacca
      541 ttgcccatct ctgaggaccc atctctggat gaggccgact gtgaggtgga atttctaacc
      601 agtgcagata cagacttcct tttagaagac tgcgtgcctt ccgatttcaa atacgatgtt
      661 ccctggcagg cgaagcttcc gtgggtgcgg acagatcaat tatgcgtatt ttgacagccc
      721 aactgtttct gtggcagatc ttagctgtgc atctgaccag aacagagttg t
//
