LOCUS       sequence-273736-        4952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Same as pLz47 but with randomized N*-peptide sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz55_polyPro_random_Npeptide
FEATURES             Location/Qualifiers
     source          1..4952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     623..642
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     726..745
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        726..744
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             776..798
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             817..834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             835..855
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(1207..1225)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1221..1268
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1634..1639,1640..1656,1657..1662))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1689..1707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1775..1879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1880..1948,1949..2740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2098..2117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2911..3499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3400..3419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3653..3670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3685..3824
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3750..3769)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3910..3932
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3926..4117)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
       61 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga
      121 cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
      181 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
      241 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
      301 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
      361 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
      421 tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
      481 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
      541 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
      601 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
      661 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc
      721 gaaattaata cgactcacta tagggacacc acaacggttt ccctctagaa ataattttgt
      781 ttaactttaa gaaggagata taccatgtcc tggaagcacc atcaccatca ccatgaaaac
      841 ctgtacttcc agtccgcagc tgttggtggg ggatctggag gtgggtctgg tggacctccc
      901 ccgcctccac cgccaccgcc agctcctgca cctgcgcctg ctcctccgcc accccctcca
      961 ccgccacctg ctcctgctcc tgctccggca ccaccacctc cgcctccgcc acctggaggt
     1021 ggatcaggag gtggttcagg tggaggacgc cgcaaagggt tgcgtgccgc tggacgtgcc
     1081 cttcaggtag agcaagcaga tagccgtgca ggtggcggta gcggaggtgg tagtggtgga
     1141 agcggttaag cggttaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     1201 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     1261 gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg
     1321 atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag
     1381 cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct
     1441 agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc
     1501 ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg
     1561 acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt
     1621 gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag
     1681 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1741 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1801 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1861 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1921 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1981 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2041 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2101 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     2161 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2221 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     2281 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     2341 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     2401 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     2461 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2521 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2581 agccggtgag cgtggctctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2641 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2701 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2761 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2821 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3541 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3601 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3661 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3721 tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3781 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     3841 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3901 tgaccgcctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3961 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
     4021 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     4081 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     4141 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
     4201 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
     4261 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
     4321 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
     4381 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
     4441 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
     4501 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
     4561 ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc
     4621 ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca
     4681 cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt
     4741 gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg
     4801 gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc
     4861 cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag
     4921 agccgcgagc gatccttgaa gctgtccctg at
//
