LOCUS       sequence-273746-       12348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C,
            Fritz-Laylin LK, Buchler NE
  TITLE     Genetic transformation of Spizellomyces punctatus, a resource for 
            studying chytrid biology and evolutionary cell biology.
  JOURNAL   Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.
  PUBMED    32392127
REFERENCE   2  (bases 1 to 12348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-11"; volume: "9. pii: 52741. doi"; pages: " 
            10.7554/eLife.52741"
COMMENT     Sequence Label: pGI3EM18
FEATURES             Location/Qualifiers
     source          1..12348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1101..2443
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(2064..2111)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     2484..2502
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2705..2920
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2921..3724
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    3891..3915
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4096..4118
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4110..4127
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4111..4127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4426..5451
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             4426..5451
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             5467..6897
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     terminator      6919..7106
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6974..6998)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7121..7137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7121..7137)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7134..7156)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7145..7161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7169..7175,7176..7193,7194..7199))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7214..7235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7412..7436
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(7718..7737)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7729..8544
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             7729..8544
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7802..7821)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8639..9227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9128..9147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9381..9398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9413..9553
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      9897..10091
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10157..11230)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11659..12288)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
       61 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
      121 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
      181 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
      241 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
      301 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
      361 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
      421 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
      481 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
      541 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
      601 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
      661 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
      721 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
      781 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
      841 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
      901 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
      961 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
     1021 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
     1081 agaaggaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca
     1141 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     1201 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa
     1261 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
     1321 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta
     1381 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct
     1441 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg
     1501 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt
     1561 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc
     1621 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt
     1681 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact
     1741 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta
     1801 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga
     1861 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
     1921 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat
     1981 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg
     2041 cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga
     2101 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc
     2161 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac
     2221 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta
     2281 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc
     2341 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat
     2401 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatactaa gaaaccatta
     2461 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     2521 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2581 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2641 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatacca
     2701 ccttttcaat tcatcatttt ttttttattc ttttttttga tttcggtttc cttgaaattt
     2761 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
     2821 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
     2881 actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa
     2941 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
     3001 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
     3061 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
     3121 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
     3181 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
     3241 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
     3301 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
     3361 tttgatgcta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
     3421 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
     3481 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
     3541 tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg
     3601 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
     3661 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
     3721 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
     3781 ttaattatat cagttattac ctggcatgca catacaaatg gacgaacgga taaacctttt
     3841 cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc
     3901 caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc
     3961 aagctcaagc tcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4021 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4081 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttttat
     4141 gctccaagcg gagacgatga ggccgatatt acagtggctt agtagcgtgt acaatatttc
     4201 cataaaaggc tacatggtga gtttttcacg tgaccgtaat tagtcccaat taggctttat
     4261 tgtatattgc tactaagcat cgttaaatga cctgctcgaa gcttcgtcta taaaaggcgg
     4321 gcgtgtgtgc gcttcccccg ctacgtcttt ttcacaaaca ctttgcgact tgaccaagac
     4381 ctttcacccg caccactctc atccatcaca cacacaaatt aattaatggg taaaaagcct
     4441 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     4501 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     4561 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     4621 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     4681 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     4741 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     4801 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     4861 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     4921 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     4981 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5041 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5101 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5161 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     5221 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     5281 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     5341 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     5401 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga aggaggagga
     5461 ggatccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     5521 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     5581 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     5641 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     5701 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     5761 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     5821 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     5881 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     5941 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     6001 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     6061 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     6121 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc
     6181 ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag
     6241 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     6301 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     6361 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     6421 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     6481 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     6541 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     6601 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     6661 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     6721 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     6781 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     6841 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaaagg
     6901 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
     6961 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     7021 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc
     7081 gctcttattg accacacctc taccgggaat tcgtaatcat gtcatagctg tttcctgtgt
     7141 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7201 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7261 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7321 gcggtttgcg tattggctag agcaattcgg cgttaattca gtacattaaa aacgtccgca
     7381 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     7441 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     7501 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     7561 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     7621 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atccagatca
     7681 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     7741 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat
     7801 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat
     7861 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     7921 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     7981 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa
     8041 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     8101 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     8161 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     8221 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     8281 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     8341 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     8401 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     8461 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     8521 ttgatgctcg atgagttttt ctaagaatta attcatgatc ctgcatgacc aaaatccctt
     8581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8761 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     9421 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     9481 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     9541 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     9601 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     9661 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
     9721 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     9781 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     9841 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     9901 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     9961 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
    10021 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
    10081 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
    10141 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
    10201 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
    10261 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
    10321 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
    10381 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
    10441 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
    10501 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
    10561 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
    10621 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
    10681 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
    10741 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
    10801 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
    10861 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
    10921 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
    10981 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
    11041 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
    11101 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
    11161 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
    11221 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
    11281 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
    11341 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
    11401 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
    11461 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
    11521 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
    11581 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    11641 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    11701 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    11761 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    11821 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    11881 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    11941 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    12001 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    12061 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    12121 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    12181 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    12241 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    12301 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatga
//
