LOCUS       sequence-273754-       11899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derived from pAH-CTX1-rha. The Burkholderia cenocepacia 
            codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is 
            controlled by the rhamnose-inducible promoter system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Hogan AM, Rahman ASMZ, Lightly TJ, Cardona ST
  TITLE     A broad-host-range CRISPRi toolkit for silencing gene expression in 
            Burkholderia.
  JOURNAL   ACS Synth Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232.
  PUBMED    31491085
REFERENCE   2  (bases 1 to 11899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232."
COMMENT     Sequence Label: pAH-CTX1-rhadCas9
FEATURES             Location/Qualifiers
     source          1..11899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..203
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      4357..4428
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4444..4471
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(4509..4529)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4509..4527)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    5066..5113
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(5332..5337,5338..5354,5355..5360)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             5408..6598
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(5760..5779)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      7420..8008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7909..7928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            9440..9548
                     /note="incP origin of transfer"
     protein_bind    9755..9802
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9976..9995
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9976..9994
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     10019..10035
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     10020..10036
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(10080..10928)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(11002..11838)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLDQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 aaattttcga cggaaaacca cgtaaaaaac gtcgattttt caagatacag cgtgaatttt
       61 caggaaatgc ggtgagcatc acatcaccac aattcagcaa attgtgaaca tcatcacgtt
      121 catctttccc tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc
      181 aggcgctttt tagactggtc gtaatgaaat tcagcaggaa ctagttatgg acaagaagta
      241 ctcgatcggc ctcgcgatcg gcacgaattc cgtgggctgg gcggtcatca cggacgaata
      301 caaggtcccg agtaagaaat tcaaagtgct cggcaatacg gaccgccact ccattaagaa
      361 aaatctgatt ggcgcgctgc tgttcgattc gggcgaaacg gccgaagcga cccgcctcaa
      421 gcgtacggcg cgtcggcgct acacgcgccg caaaaatcgc atttgctacc tgcaggaaat
      481 cttcagcaac gaaatggcca aggtggacga tagcttcttc caccgtctgg aggagagctt
      541 cctggtcgag gaggacaaga agcacgagcg ccatccgatt ttcggtaaca tcgtcgacga
      601 agttgcgtac catgagaagt atcccacgat ctatcacctc cgcaagaaac tggttgactc
      661 caccgataaa gcggacctgc gcctcatcta cctcgccctg gcccacatga ttaagttccg
      721 cggtcacttc ctgatcgagg gcgacctgaa ccccgataat tcggacgtcg ataagctctt
      781 cattcagctg gtgcagacgt acaatcagct gttcgaagaa aaccccatta atgcgtccgg
      841 cgtcgacgcg aaggcgatcc tcagcgcgcg cctcagtaag tcgcgccgcc tggaaaacct
      901 catcgcccaa ctcccgggcg agaagaagaa cggtctcttc ggcaacctca tcgccctgtc
      961 cctgggcctc acgcccaact tcaagagcaa ctttgacctg gccgaagacg cgaagctgca
     1021 actgagcaag gatacctacg acgacgacct ggacaacctg ctggcccaga tcggcgatca
     1081 gtacgccgat ctcttcctcg ccgccaagaa cctgtccgac gcgattctgc tctccgacat
     1141 tctccgcgtc aacacggaga ttacgaaggc gccgctgtcg gccagcatga ttaagcgcta
     1201 cgacgaacac caccaggatc tgaccctgct gaaggccctc gttcgccagc aactcccgga
     1261 gaagtacaag gaaattttct tcgaccagtc gaaaaacggc tatgccggct atatcgacgg
     1321 tggcgcgtcc caagaagagt tctacaagtt cattaaaccg attctggaga agatggacgg
     1381 cacggaggag ctgctggtca agctgaaccg cgaagatctg ctgcgcaaac aacgcacctt
     1441 cgacaacggc agcatccccc accagattca cctgggcgaa ctccatgcca tcctgcggcg
     1501 ccaggaggac ttctatccgt tcctgaaaga caatcgggaa aaaatcgaaa aaattctcac
     1561 cttccgcatt ccgtactatg tcggcccgct ggcgcgcggc aacagccggt tcgcctggat
     1621 gacgcgcaag agtgaagaga ccatcacgcc ctggaacttc gaagaagtgg ttgataaggg
     1681 tgccagcgcc cagagcttca ttgaacgcat gacgaatttc gacaagaacc tgccgaacga
     1741 gaaggtgctc cccaagcact cgctcctcta tgaatacttc accgtgtaca acgagctcac
     1801 caaggtcaag tatgtcaccg agggcatgcg caagccggcc ttcctctcgg gcgagcagaa
     1861 gaaggccatt gtcgacctgc tcttcaagac gaaccgtaaa gtcaccgtga agcagctgaa
     1921 ggaggactac ttcaaaaaaa tcgaatgctt cgacagcgtg gagatttcgg gcgtggagga
     1981 ccgcttcaac gccagcctcg gtacctacca cgatctgctc aaaatcatca aggataagga
     2041 cttcctggac aacgaggaga atgaggatat tctggaagat atcgtcctga cgctgacgct
     2101 ctttgaagac cgggaaatga tcgaggagcg cctgaagacg tacgcccacc tcttcgacga
     2161 caaggtgatg aaacaactca aacgccgccg ctatacgggc tggggccgcc tgagtcgcaa
     2221 gctgatcaac ggcatccggg acaaacagag cggcaagacc attctcgact tcctgaaatc
     2281 cgacggcttc gccaaccgca acttcatgca gctcatccat gacgatagcc tgacgttcaa
     2341 ggaggacatc cagaaggcgc aggtgtcggg ccagggtgac tcgctccacg aacatattgc
     2401 caacctggca ggctccccgg caatcaagaa gggcattctg cagaccgtca aagttgtgga
     2461 cgaactcgtc aaagtgatgg gtcgccataa gcccgagaac atcgtgatcg aaatggcccg
     2521 cgagaaccag acaacccaga agggtcagaa gaactcccgc gagcgcatga agcgcattga
     2581 agaaggcatc aaagaactgg gcagccaaat cctcaaggag cacccggttg agaataccca
     2641 gctgcagaac gaaaagctct acctgtacta cctgcagaac ggccgcgaca tgtatgttga
     2701 ccaggagctg gacattaatc gtctctcgga ttacgacgtc gatgccatcg tgccccagtc
     2761 cttcctcaaa gacgactcga ttgacaacaa agtgctcacc cgctccgaca aaaaccgcgg
     2821 caagagcgac aacgtgccga gtgaggaagt ggtcaaaaag atgaagaatt actggcgcca
     2881 gctgctgaac gcgaagctca ttacgcagcg caagtttgac aacctgacca aagccgaacg
     2941 cggtggcctc agcgagctgg acaaggcagg cttcattaag cgccagctcg tggaaacgcg
     3001 ccagatcacc aagcacgtcg cgcagatcct cgacagccgg atgaatacga agtacgacga
     3061 aaatgataag ctgatccgcg aagtgaaggt cattaccctc aagtcgaagc tcgtctcgga
     3121 tttccgcaaa gacttccagt tttacaaggt tcgcgaaatt aacaactatc accacgcaca
     3181 cgacgcatat ctcaacgcgg tcgtcggcac cgcgctgatt aagaaatatc ccaagctgga
     3241 atcggaattc gtctacggcg actataaggt gtatgacgtg cggaagatga ttgcgaaaag
     3301 cgaacaggaa atcggcaagg ccacggccaa gtacttcttc tactcgaata tcatgaattt
     3361 ctttaaaacc gagattacgc tcgccaacgg cgagatccgc aaacgcccgc tcatcgagac
     3421 gaacggcgaa acgggtgaaa tcgtgtggga taagggccgc gatttcgcga ccgtgcgcaa
     3481 ggtgctctcg atgccgcaag tcaatatcgt caagaagacc gaagtccaga cgggcggttt
     3541 ctacaaggaa agcatcctgc cgaagcgcaa ctcggacaaa ctcatcgccc gcaagaaaga
     3601 ttgggatccc aagaagtatg gcggcttcga ctcgcccacg gtggcgtatt cggtgctcgt
     3661 ggtcgcgaag gtggaaaagg gcaagtccaa aaaactcaaa tcggttaaag agctgctcgg
     3721 catcaccatt atggagcgct ccagcttcga gaaaaacccg atcgacttcc tcgaagccaa
     3781 gggctacaaa gaggtgaaaa aggatctgat tatcaagctg ccgaaatatt cgctgttcga
     3841 actcgaaaat ggccgcaagc gcatgctggc gtcggcgggt gagctccaaa aaggtaacga
     3901 actggcgctg ccgagcaaat acgtcaactt tctgtacctc gccagccact acgaaaagct
     3961 caagggttcg ccggaggata acgagcaaaa gcaactcttt gttgaacagc ataaacacta
     4021 cctggatgag atcattgaac agatctcgga gttctccaag cgcgtcattc tcgcggacgc
     4081 aaacctggat aaagtcctga gcgcctacaa caaacaccgc gataagccga tccgcgaaca
     4141 ggcggagaac atcatccacc tgttcaccct gaccaacctg ggcgcccccg cggccttcaa
     4201 gtatttcgac acgaccatcg accgcaagcg ctataccagc accaaggagg ttctcgacgc
     4261 cacgctgatc caccagagca ttaccggcct ctacgaaacg cgtatcgacc tctcgcagct
     4321 gggtggtgac tgactcgagt gaggatctcc aggcatcaaa tgaaacgaaa ggctcagtcg
     4381 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     4441 actggctcac cttcgggtgg gcctttctgc gtgcggccgc caccgcggtg gagctccagc
     4501 ttttgttccc tttagtgagg gttaattgcg cgggtggatg accttttgaa tgacctttaa
     4561 tagattatat tactaattaa ttggggaccc tagaggtccc cttttttatt ttaaaaattt
     4621 tttcacaaaa cggtttacaa gcataaagct aattcagcaa ggacggcccc gacatcgtag
     4681 gtctgctgaa tcaggcgctg aaactttcct gaagcaacga aaaaggggcc ctgcggggcc
     4741 ccttttttct gcgcctcgtt cccagtttgt tcccaaatcg ttcactcctg agagtgggtc
     4801 gaatggaaac atcaatgaaa acagcaggtt atatggcgga ggcggtgaga ttcgaactca
     4861 tatgaaacgc cccaacctcg ctggaggccg cattattcct gggccctact gtccattttc
     4921 actcgcgttt gttcccagag tgttcccagc gtgtgcgaga gagcctggaa gcctgccgtc
     4981 aaacctgccg tcccagggca acagccggat ttcatgttga cccattgaaa tccattgctg
     5041 ttgacaaagg gaatcagggg atcttgaagt tcctattccg aagttcctat tctctagaaa
     5101 gtataggaac ttcagagcgc ttttgaagct aattcgctgc attaatgaat cggccaacgc
     5161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5281 tccacagaat caggggataa cgcaggaaag aacatgaatt aattctcatg tttgacagct
     5341 tatcatcgat tagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc
     5401 accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc
     5461 tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata ttgtccattc
     5521 cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct
     5581 atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc
     5641 ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat
     5701 tctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     5761 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     5821 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     5881 cttacatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     5941 cttcctaatg caggagtcgc ataagggaga gcgccgaccc atgcccttga gagccttcaa
     6001 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     6061 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     6121 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     6181 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     6241 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     6301 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     6361 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     6421 atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat
     6481 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
     6541 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
     6601 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
     6661 ttgcggagaa ctgtgaatgc gcaaaccaac ccttgactca ccagtcacag aaaagcatct
     6721 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6781 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6841 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6901 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6961 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7021 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7081 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7141 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7261 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7321 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7381 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7441 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7561 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7621 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7681 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7741 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7861 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7921 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7981 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8041 ggccttttgc tggccttttg ctcacatcca gcttggtagc ctgaacgctt ggtgtggatc
     8101 aggggaacca gatggcagac ggagtcgagg tacgcggaaa acggatcagg atctacttcc
     8161 gctaccaggg cgagctgtgt agggagtcca taccagggga cgcaacgccc gagaatatag
     8221 ccaatgccga acagctggcc ggcatcatca actacgagat caagcaagga gtcttcagct
     8281 acagccgaca cttcccggac tcaccgcggg tgaaaagcaa caccttgggc cattacatcg
     8341 acctctggct cgacatcaaa cgcaaccaga ttgccgccag cggcttccgc ggctacacca
     8401 gccgcgtgga aacccacatc cgacctcgct ggggtgacag ccaggccgac agcatcgacc
     8461 acctggatat tcaagactgg gtgcagaaca cgctcatgcc caagctgcac aacaagacgg
     8521 tgcgagagat cgtcagcaac ctgcggcaga tcttccggct gtatcgaact cggaaccgga
     8581 gcgcgcacga cccaacagac ggcatcgtga tcactcttcc ggatgcggat gacccagacc
     8641 cattcactcg tgaggaaatc gacctcatcc tcggtactga aaccgcgcgc atcggtgagc
     8701 tgaacctggc ggagttcatg atctggagcg gcccccgcgt cagcgaggcc atagctctgg
     8761 cctgggaaga tgtcgatttg gacacgggga ccgtggtttt ccgccgcgct cgtgtacgca
     8821 gccagtacaa ggtcaccaag actcgccgat cgacgaggaa ggtccagcta ctggccccgg
     8881 cgctgcgcgc actccagcag caggccaagc taaccagacg ccttccgccc gtacagatcg
     8941 aggtgataga ccgcgacaac cggaccagaa agcctcaacg ggtacgcttc gtgttccaca
     9001 acagtgctag cggggcggcg tactccacgt cggatacgct gcgcaacggc tggtggcatg
     9061 gccacctcag gaatgcgggc gtccgctcgc gtggtcccaa tcagtgccgc cacaccttcg
     9121 ccagccagat gctcagcagc ggcatcgcca ctcccgaatg gatcgcggac cagatggggc
     9181 acacctcgac ggcgatgatc ttcaagcatt acgccaagtg gatcagcaag gacggccccg
     9241 acatcgtagg tctgctgaat caggcgctga aactttcctg aagcaacgaa aaaggttaag
     9301 gtatactttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     9361 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     9421 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     9481 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     9541 gctggccgat aagctctagc atgttctttc ctgcgttatc ccctgattct gtggataacc
     9601 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9661 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9721 ggccgattca ttaatgcagc gaattgggga tcttgaagta cctattccga agttcctatt
     9781 ctctagaaag tataggaact tcagagcgct tttgaagctg atgtgcttaa aaacttactc
     9841 aatggaatta gctttatgct tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg
     9901 ggacctctag ggtccccaat taattagtaa tataatctat taaaggtcat tcaaaaggtc
     9961 atccaccggc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc
    10021 gaggtcgacg gtatcgataa gcttatgtca actgggttcg tgcgagctca tcgatgcatt
    10081 taatctttct gcgaattgag atgacgccac tggctgggcg tcatcccggt ttcccgggta
    10141 aacaccaccg aaaaatagtt actatcttca aagccacatt cggtcgaaat atcactgatt
    10201 aacaggcggc tatgctggag aagatattgc gcatgacaca ctctgacctg tcgcagatat
    10261 tgattgatgg tcattccagt ctgctggcga aattgctgac gcaaaacgcg ctcactgcac
    10321 gatgcctcat cacaaaattt atccagcgca aagggacttt tcaggctagc cgccagccgg
    10381 gtaatcagct tatccagcaa cgtttcgctg gatgttggcg gcaacgaatc actggtgtaa
    10441 cgatggcgat tcagcaacat caccaactgc ccgaacagca actcagccat ttcgttagca
    10501 aacggcacat gctgactact ttcatgctca agctgaccga taacctgccg cgcctgcgcc
    10561 atccccatgc tacctaagcg ccagtgtggt tgccctgcgc tggcgttaaa tcccggaatc
    10621 gccccctgcc agtcaagatt cagcttcaga cgctccgggc aataaataat attctgcaaa
    10681 accagatcgt taacggaagc gtaggagtgt ttatcgtcag catgaatgta aaagagatcg
    10741 ccacgggtaa tgcgataagg gcgatcgttg agtacatgca ggccattacc gcgccagaca
    10801 atcaccagct cacaaaaatc atgtgtatgt tcagcaaaga catcttgcgg ataacggtca
    10861 gccacagcga ctgcctgctg gtcgctggca aaaaaatcat ctttgagaag ttttaactga
    10921 tgcgccaccg tggctacctc ggccagagaa cgaagttgat tattcgcaat atggcgtaca
    10981 aatacgttga gaagattcgc gttattgcag aaagccatcc cgtccctggc gaatatcacg
    11041 cggtgaccag ttaaactctc ggcgaaaaag cgtcgaaaag tggttactgt cgctgaatcc
    11101 acagcgatag gcgatgtcag taacgctggc ctcgctgtgg cgtagcagat gtcgggcttt
    11161 catcagtcgc aggcggttca ggtatcgctg aggcgtcagt cccgtttgct gcttaagctg
    11221 ccgatgtagc gtacgcagtg aaagagaaaa ttgatccgcc acggcatccc aattcacctc
    11281 atcggcaaaa tggtcctcca gccaggccag aagcaagttg agacgtgatg cgctgttttc
    11341 caggttctcc tgcaaactgc ttttacgcag caagagcagt aattgcataa acaagatctc
    11401 gcgactggcg gtcgagggta aatcattttc cccttcctgc tgttccatct gtgcaaccag
    11461 ctgtcgcacc tgctgcaata cgctgtggtt aacgcgccag tgagacggat actgcccatc
    11521 cagctcttgt ggcagcaact gatccagccc ggcgagaaac tgaaatcgat ccggcgagcg
    11581 atacagcaca ttggtcagac acagattatc ggtatgttca tacagatgcc gatcatgatc
    11641 gcgtacgaaa cagaccgtgc caccggtgat ggtatagggc tgcccattaa acacatgaat
    11701 acccgtgcca tgttcgacaa tcacaatttc atgaaaatca tgatgatgtt caggaaaatc
    11761 cgcctgcggg agccggggtt ctatcgccac ggacgcgtta ccagacggaa aaaaatccac
    11821 actatgtaat acggtcatac tggcctcctg atgtcgtcaa cacggcgaaa tagtaatcac
    11881 gaggtcaggt tcttacctt
//
