LOCUS       sequence-273767-        6165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz33_CsoS2_NTD_GFP
FEATURES             Location/Qualifiers
     source          1..6165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..73)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      69..116
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(482..487,488..504,505..510))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(537..555)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        623..727
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(728..796,797..1588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(946..965)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1759..2347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2248..2267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2501..2518
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2533..2672
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2598..2617)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2758..2780
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2774..2965)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4423..4442
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4526..4545
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4526..4544
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4576..4598
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4608..4625
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4626..4646
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gttaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
       61 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      121 aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
      181 atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
      241 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
      301 tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
      361 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
      421 ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
      481 cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct
      541 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      601 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      661 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      721 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      781 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      841 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      901 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      961 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1021 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1081 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1141 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1201 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1261 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1321 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1381 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1441 tggctctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1501 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1561 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1621 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1681 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1741 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1801 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1861 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     1921 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1981 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2041 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2101 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2161 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2221 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2281 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2341 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2401 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2461 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2521 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2581 caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     2641 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     2701 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgcctccg
     2761 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     2821 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     2881 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     2941 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     3001 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     3061 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     3121 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     3181 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     3241 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     3301 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     3361 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     3421 tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga
     3481 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
     3541 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc
     3601 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
     3661 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
     3721 aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga
     3781 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     3841 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     3901 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
     3961 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     4021 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     4081 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     4141 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     4201 gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa
     4261 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     4321 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     4381 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     4441 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     4501 gatcgagatc tcgatcccgc gaaattaata cgactcacta tagggacacc acaacggttt
     4561 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgcac catcaccatc
     4621 accatgaaaa cctgtacttc cagtcccctt cacagtcagg aatgaatcct gccgacctga
     4681 gcggactctc tggcaaggaa ctggcacgcg cacgccgcgc tgcactatcc aagcaaggga
     4741 aagcagctgt ttctaataaa acggctagcg taaaccgtag cactaaacag gcggcatctt
     4801 cgatcaatac aaatcaggtg cgctcttctg taaatgaagt gcccactgat taccaaatgg
     4861 cggatcaatt gtgctctacg attgatcatg ctgactttgg taccgaaagc aatcgcgtta
     4921 gagatctctg ccgtcaacgc agagaggcac tatcaactat cggtaaaaaa gcggttaaaa
     4981 ccaacggcaa gccgtcgggt cgcgttcgac cacagcaatc agtggttcac aacgacgcaa
     5041 tgatcgaaaa tgccggtgat actaaccaat catcgtccac ttcattaaat aatgaacttt
     5101 ccgaaatctg ctccatagca gacgacatgc cggagcgttt tggttcacaa gccaaaaccg
     5161 tccgtgatat ctgccgtgca cgccgtcaag cgctctctga gcgtggaact cgcgccgtgc
     5221 cgccaaagcc gcaatctcaa ggtggtccag gacgcaatgg ctatcaaatt gatggatacc
     5281 tagataccgc acttcatggc cgcgatgccg ccaagcgcca ccgtgaaatg ctctgtcaat
     5341 acggccgcgg cacagcacct tcctgcaagc caacaggccg tgtcaaaaat tctgtacagt
     5401 cgggcaacgc agcgccaaaa aaggtttcag gtagcggaag cggcggttct aaaggtgaag
     5461 aactgttcac cggtgttgtt ccgatcctgg ttgaactgga tggtgatgtt aacggccaca
     5521 aattctctgt tcgtggtgaa ggtgaaggtg atgcaaccaa cggtaaactg accctgaaat
     5581 tcatctgcac taccggtaaa ctgccggttc catggccgac tctggtgact accctgacct
     5641 atggtgttca gtgtttttct cgttacccgg atcacatgaa gcagcatgat ttcttcaaat
     5701 ctgcaatgcc ggaaggttat gtacaggagc gcaccatttc tttcaaagac gatggcacct
     5761 acaaaacccg tgcagaggtt aaatttgaag gtgatactct ggtgaaccgt attgaactga
     5821 aaggcattga tttcaaagag gacggcaaca tcctgggcca caaactggaa tataacttca
     5881 actcccataa cgtttacatc accgcagaca aacagaagaa cggtatcaaa gctaacttca
     5941 aaattcgcca taacgttgaa gacggtagcg tacagctggc ggaccactac cagcagaaca
     6001 ctccgatcgg tgatggtccg gttctgctgc cggataacca ctacctgtcc acccagtcta
     6061 aactgtccaa agacccgaac gaaaagcgcg accacatggt gctgctggag ttcgttactg
     6121 cagcaggtat cacgcacggc atggatgagc tctacaaata aagcg
//
