LOCUS       sequence-273768-       13243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Halothiobacillus neapolitanus carboxysome operon with full length 
            CsoS2. For heterologous expression of carboxysomes in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 13243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz37_CB_operon_CsoS2_N1-N4
FEATURES             Location/Qualifiers
     source          1..13243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5..91
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      183..210
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    232..251
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(266..271,272..289,290..296)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    304..320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        8734..8835
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8836..9495
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8902..8921)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        9560..9637
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9638..10720
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9657..9676)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      complement(11705..12293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11785..11804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
       61 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
      121 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
      181 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta ctctagtttc gaattgtgag
      241 cgctcacaat ttcgaaaccc caggcttgac actttatgct tccggctcgt ataatgtgtg
      301 gaattgtgag cggataacaa tttcagctag gtgcgcgaat cccccatcct ttcaggagga
      361 actcatggca gttaaaaagt atagtgctgg tgtaaaagaa taccggcaga cctattggat
      421 gccggaatac acaccgttgg attccgacat ccttgcatgc ttcaaaatca ccccacaacc
      481 gggtgttgat cgcgaagaag ccgcagccgc ggttgcagca gaatcttcaa ccggcacatg
      541 gaccaccgtg tggaccgatt tgctgaccga catggactac tacaaaggcc gtgcctatcg
      601 cattgaagac gtacccggtg acgatgcggc attctatgcc tttatcgcct acccaatcga
      661 cctgttcgaa gaagggtcag ttgttaacgt gtttacctca ctggttggta acgtgttcgg
      721 cttcaaagcg gtacgcggcc tgcgtctgga agatgttcgc ttcccactcg cctacgttaa
      781 aacctgtggc ggcccaccgc acggtattca agtcgaacgc gacaagatga acaaatatgg
      841 tcgcccactg ttgggttgca ccatcaagcc aaaacttggt ttgtctgcga aaaactacgg
      901 ccgtgccgta tacgagtgcc tccgtggcgg cctcgacttc actaaagatg atgaaaacat
      961 caattctcag ccgttcatgc gctggcgcga tcgcttcttg ttcgtacaag acgcgaccga
     1021 aactgctgaa gcccaaaccg gcgaacgcaa aggccattac ctcaacgtaa cggcgccaac
     1081 tcctgaagaa atgtacaagc gcgcagaatt cgccaaagaa attggcgcgc caatcattat
     1141 gcacgactac atcaccggtg gcttcacggc caacactggc ttggccaagt ggtgtcaaga
     1201 caacggcgta ctgctgcaca tccaccgtgc gatgcatgcg gttatcgacc gtaacccgaa
     1261 ccacggtatt cacttccgtg ttctgaccaa gattctgcgt ttgtcgggtg gcgatcacct
     1321 gcacaccggt accgttgtcg gcaaactgga aggcgaccgt gcctctactc tgggctggat
     1381 tgatttgctc cgcgaatcgt ttatccctga agatcgctcg cgcggtatct tcttcgatca
     1441 agactggggt tcaatgccag gcgtattcgc tgtggcctct ggtggtattc acgtatggca
     1501 catgcctgcg ctggttaaca tctttggtga cgactctgtc ctccaattcg gtggcggtac
     1561 gctgggtcat ccatggggca acgctgccgg tgctgctgcc aaccgtgttg ctctggaagc
     1621 ctgcgtagaa gcgcgtaacc aaggccgcga tatcgaaaaa gaaggcaaag aaattctgac
     1681 tgctgctgca cagcacagcc cagaactgaa gattgccatg gaaacttgga aagagatcaa
     1741 attcgaattt gacactgtcg acaaactcga cactcaaaat cgttgatccc tcgtaccaca
     1801 caacatacta aggtgagtaa ccatggctga aatgcaggat tacaagcaaa gcctcaaata
     1861 tgagactttc tcttatcttc cacccatgaa cgcggaacgc atccgcgctc aaatcaagta
     1921 cgcaattgct caaggctgga gccccggcat tgagcacgta gaagtgaaaa actccatgaa
     1981 ccaatattgg tacatgtgga aacttccctt cttcggcgaa caaaatgtcg acaacgtgtt
     2041 ggctgaaatt gaagcgtgtc gtagtgcgta tccaacacac caggtcaaac tggtggctta
     2101 tgacaactat gcgcaaagct taggtctggc cttcgtggtc taccgcggca actaagtcaa
     2161 gtctgtcatt gcgctgtgcg tttctctacg cacagcactt attcaagatg gggtcaaaca
     2221 tgccttcaca gtcaggaatg aatcctgccg acctgagcgg actctctggc aaggaactgg
     2281 cacgcgcacg ccgcgctgca ctatccaagc aagggaaagc agctgtttct aataaaacgg
     2341 ctagcgtaaa ccgtagcact aaacaggcgg catcttcgat caatacaaat caggtgcgct
     2401 cttctgtaaa tgaagtgccc actgattacc aaatggcgga tcaattgtgc tctacgattg
     2461 atcatgctga ctttggtacc gaaagcaatc gcgttagaga tctctgccgt caacgcagag
     2521 aggcactatc aactatcggt aaaaaagcgg ttaaaaccaa cggcaagccg tcgggtcgcg
     2581 ttcgaccaca gcaatcagtg gttcacaacg acgcaatgat cgaaaatgcc ggtgatacta
     2641 accaatcatc gtccacttca ttaaataatg aactttccga aatctgctcc atagcagacg
     2701 acatgccgga gcgttttggt tcacaagcca aaaccgtccg tgatatctgc cgtgcacgcc
     2761 gtcaagcgct ctctgagcgt ggaactcgcg ccgtgccgcc aaagccgcaa tctcaaggtg
     2821 gtccaggacg caatggctat caaattgatg gatacctaga taccgcactt catggccgcg
     2881 atgccgccaa gcgccaccgt gaaatgctct gtcaatacgg ccgcggcaca gcaccttcct
     2941 gcaagccaac aggccgtgtc aaaaattctg tacagtcggg caacgcagcg ccaaaaaagg
     3001 ttgaaaccgg tcacacccta tccggcggat ctgttacggg cacgcaagtg gatcgtaaat
     3061 ctcatgtgac tggaaacgag ccgggcactt gccgagcagt cacgggcacc gagtacgtag
     3121 gtactgagca attcacctct ttttgcaata ccagccccaa gccaaatgcg acgaaggtca
     3181 atgtgaccac aacggctcgt ggtcgccctg ttagcggtac ggaagtttca cggaccgaga
     3241 aggtaactgg caacgaatcc ggtgtctgcc gtaacgttac cggcaccgaa tacatgagta
     3301 atgaagctca cttttctcta tgtggaacag ccgcaaagcc ttcacaagcg gataaagtca
     3361 tgttcggcgc cacagcacga acgcatcaag tggtcagtgg cagtgatgaa ttcaggccct
     3421 cttctgttac gggtaacgaa tcgggtgcaa aacgcacaat taccggctcg cagtacgcag
     3481 acgaaggtct tgcgcgactc acgatcaacg gagcacctgc aaaagtagcc agaacccaca
     3541 cctttgcggg ctctgacgtt accggcacgg aaatcggtcg ctctactcgc gtaactggtg
     3601 atgaaagcgg ttcgtgtcgt tcaatctcag gcaccgagta tctcagtaac gagcaattcc
     3661 aatccttttg tgacacaaaa cctcaacgca gcccgttcaa ggttggccaa gatcgcacga
     3721 acaagggtca gtctgtgact ggtaacttgg ttgatcgttc cgaactggtt acaggtaacg
     3781 aaccaggttc atgctctcgg gttacaggct ctcagtatgg ccaaagcaaa atctgcggtg
     3841 gtggcgtggg aaaagtgcgc tcaatgcgca cccttcgcgg cacctcagta tctggccaac
     3901 agctagatca tgccccaaag atgtccggtg acgagcgcgg cgggtgcatg cccgtcaccg
     3961 gtaatgagta ctacggtcgt gaacatttcg aaccgttttg tacgagcacc ccagagcccg
     4021 aagctcaatc aactgaacaa tcattgacct gtgaaggaca aattattagc ggcacttcag
     4081 ttgacgccag tgatttggtc acaggaaatg aaatcggtga acagcaactc atcagcggtg
     4141 acgcctatgt tggcgcgcag cagacaggtt gccttcccac tagtccacgc ttcaaccaaa
     4201 ctggcaatgt tcagtcaatg ggttttaaga acaccaatca gccagaacaa aactttgcac
     4261 caggtgaagt aatgcctact gactttagta ttcaaacccc agctcgctcg gctcagaatc
     4321 gcattacagg taacgacatt gcgccctcag gtcgcattac aggccctggt atgctggcaa
     4381 ccggcttgat tacaggaacc cccgaattca ggcacgctgc gcgcgagttg gttggttctc
     4441 cacaacccat ggcaatggcc atggccaacc gtaataaggc tgctcaagca cctgttgtgc
     4501 agccagaagt ggttgcaact caggaaaagc ctgagttggt atgtgcacca agaagcgatc
     4561 aaatggatcg tgtgagtggc gaaggcaaag aacgttgcca catcactggc gatgactggt
     4621 cagttaacaa gcacatcacc ggtacagccg gtcaatgggc gagtggtcgc aacccttcca
     4681 tgcgcggtaa tgcgcgtgtg gtcgaaacca gcgcgtttgc caatcgcaat gtgccaaaac
     4741 ctgaaaagcc gggctccaag atcacgggca gtagtggtaa tgacacccaa ggtagtctga
     4801 tcacttactc cggcggcgcg cgcggttgag cgttaagtaa agtgtaacga tcatgaacac
     4861 ccgtaacaca cgaagcaagc aacgcgcacc gtttggtgtt agctcatcag tcaaacctcg
     4921 gcttgacttg attgagcaag caccaaaccc tgcctatgac cgccatcctg cttgcataac
     4981 gttgcctgag cgtacctgcc ggcacccgtt aactgacctt gaagccaacg aacaactggg
     5041 tcgttgcgag gatagcgtca agaaccgttt tgatcgcgtt atccctttct tgcaagttgt
     5101 tgctggcatt cctcttggtc tggattatgt tacccgtgtt caagaattag cccagtcgtc
     5161 gcttggacat acgctgcccg aagaattact caaagataat tggatcagtg ggcacaattt
     5221 aaaaggcatt tttggctacg cgactgctaa agcactaaca gcagccacgg aacaattcag
     5281 tcgtaaaata atgtctgaga aggacgattc cgcatcggcc attggcttct tcctggattg
     5341 cgggttccac gcagtggaca taagcccttg tgccgatggt cgtctcaaag ggttactgcc
     5401 ttacatattg cgtttgcccc ttacggcatt cacctatcgt aaagcctacg caggctcgat
     5461 gttcgatatt gaagatgatc tggcacagtg ggagaaaaat gaactccgcc gttatcgtga
     5521 aggtgttcca aatacagcgg atcagccaac acgatacctg aaaattgctg tgtatcattt
     5581 cagcacctct gacccgacac actctggctg cgcggcacac ggcagtaatg atcgtgcagc
     5641 actggaagcg gctttaaccc agctgatgaa attcagagaa gcggttgaaa atgcccattg
     5701 ctgcggcgca agtatcgata ttttactgat tggcgttgat acggatacgg atgccattcg
     5761 cgttcatatt ccggatagca aaggtttttt gaatccgtat cgttatgttg acaacacagt
     5821 aacttatgcg caaacactac atctggcgcc ggatgaggct cgtgtgatta ttcacgaagc
     5881 aattctcaac gcaaaccgca gcgatggttg ggctaaagga aatggagtag ccagcgaggg
     5941 gatgcgtcgt tttattggtc agcttttgat caacaacctc tcgcaaatcg attacgtagt
     6001 aaatcgtcat ggtggtcgct atccacccaa tgatattggt catgctgagc gatatatcag
     6061 tgttggtgat ggttttgatg aagttcaaat ccggaattta gcctactacg cgcatttgga
     6121 tacggttgaa gaaaatgcga ttgatgtgga tgtgggaatc aaaattttca ccaaacttaa
     6181 tttgagtcga ggtttaccga ttccgattgc catccactat cgctatgacc ccaatgttcc
     6241 aggctccaga gaaagaaccg tggtcaaagc aagacggata tacaacgcca ttaaagagcg
     6301 gttctcatcc ttggatgagc agaatctatt gcagtttcgt ttgagcgttc aggcgcagga
     6361 tatcggaagc ccgattgaag aggttgcatc cgcatgaaaa tcatgcaagt tgagaaaacg
     6421 ttggtttcaa caaaccgtat tgctgatatg ggtcacaaac cactattagt ggtatgggag
     6481 aaaccgggcg cgcccaggca ggtcgccgtg gatgcgattg gctgcatacc gggcgattgg
     6541 gttttgtgcg ttgggtcatc ggcagcacga gaggctgcag gaagcaagtc ttatccctct
     6601 gatttgacga ttatcgggat tattgatcag tggaatggtg agtaatggaa gtaatgcgcg
     6661 ttcgttccga cctaatcgca acacgcagga ttcccggtct taaaaatatc tctttgcgtg
     6721 tcatggagga tgctacgggt aaggtcagtg tcgcttgcga tcccattggc gttcccgagg
     6781 gatgttgggt ctttacgatt agcggctctg ccgctcggtt tggcgtgggt gattttgaga
     6841 ttctcacgga tttgacgatt ggtggcatca tcgatcactg ggtaacttga ccatcgctag
     6901 atgagttgat tttgaatgag tctttattga ggagagaaga aatggcagca gtaacaggta
     6961 ttgcactggg tatgattgaa acacgtggtc tggttccagc gattgaagct gccgatgcca
     7021 tgaccaaggc cgccgaagta cgtttggttg gccgtcaatt tgttggtggt ggttacgtga
     7081 ccgttttggt ccgtggtgaa accggtgccg tcaacgcagc agttcgtgcg ggcgctgatg
     7141 cctgcgaacg agtcggcgat ggtctggtcg cggcgcatat cattgcccgt gtccattccg
     7201 aagtcgaaaa catcctgccg aaagcccctg aagcttaagg attgggaaag acgaaccggc
     7261 gcaggcttgt tccggttctt tgcataaagt gacagcttag gagtttattt aaatggctga
     7321 tgtaactggt attgctctgg gtatgatcga aacacgtggc ttagttcctg cgattgaagc
     7381 agcggacgcc atgactaaag cggctgaagt gcgtttggtc ggtcgtcaat ttgttggtgg
     7441 cggttacgtc accgtattgg ttcggggcga aacaggcgct gttaacgccg ctgttcgtgc
     7501 tggcgccgat gcttgcgaac gtgttggtga tggcttggtt gctgcgcaca tcattgcgcg
     7561 tgtccactca gaagtagaaa acattctgcc taaggcgcca caagcctaag tcagatattc
     7621 ctaagacggc tcacttcggc gacacccgtc gccgaaaccg tctaaccaaa tttctgggct
     7681 cgttctgaac gtgctcaaca ctagttttag aggatctgtt atggcaacga ctcacggtat
     7741 tgccctgggc atgattgaaa cacgaggatt ggttcctgcc attgaagccg cagatgccat
     7801 gaccaaagcg gcggaagtcc gtctggtcgg acgatcattt gttggcggcg gttacgtgac
     7861 cgtaatggtt cgtggtgaga caggtgcagt aaatgctgcc gttcgtgcgg gtgctgacgc
     7921 ctgtgaacgt gttggcgatg gcctggttgc tgcgcacatc attgcgcgcg ttcattctga
     7981 agttgagatc atcctacccg agacgcccga agactcagat tccgcgtggt gtatcgcaaa
     8041 tctgaatagc taatgtctag tagggaagat gcgcatgaac aacattgatt tgcgcgtcta
     8101 ttcgtttatc gattcgcttc aaccgcagct tgcatcctat cttgcgacat catcgcaagg
     8161 ctttcttccc gttccgggtg atgcctgctt gtggattgaa gtcgcgccgg gcatggccgt
     8221 ccatcgcctc agtgatattg cgctaaaggc cacgaacgtt cgtctcggcg aacaggtagt
     8281 cgagcgtgct tttggctcga tggaaattca ttaccgaaac caaagcgacg ttctcgcatc
     8341 cggtgaggcc gttttaagag aaatcaatca cgcgcaagaa gatcgtctgc cttgtcgcat
     8401 cgcatggaaa gagatcattc gagcgattac ccccgatcat gccaccttga tcaatcgcca
     8461 gttgcgtaaa ggctctatgc tgttgccggg caaaagcatg ttcatccttg aaacagaacc
     8521 ggcaggttat attgttcagg ctgccaacga ggccgagaaa gcagctcatg tgactctgat
     8581 cgatgtacgt gcttttggta actttggtcg cctgaccatg atgggcagcg aagcggaaac
     8641 cgaagaagcc atgcgggcgg ctgaggccac aatcgcaagc atcaacgcgc gtgcgcgtcg
     8701 cgctgaaggg ttctaagcgg ccgcgtagga tccgatcggc acgtaagagg ttccaacttt
     8761 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
     8821 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     8881 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     8941 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     9001 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg
     9061 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     9121 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     9181 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     9241 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     9301 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac tatgggcaaa
     9361 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     9421 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     9481 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttgaattcc tactagtcga
     9541 agcggcatgc atttacgttg acaccatcga atggtgcaaa acctttcgcg gtatggcatg
     9601 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
     9661 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
     9721 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     9781 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     9841 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     9901 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     9961 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
    10021 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
    10081 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
    10141 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
    10201 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
    10261 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
    10321 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
    10381 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
    10441 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
    10501 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
    10561 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
    10621 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10681 gctggcacga caggtttccc gactggaaag cgggcagtga tcccacagcc gccagttccg
    10741 ctggcggcat tttaactttc tttaatgaat ctagtgacaa gccggggcag acgtgagccg
    10801 tagtcccgtc gccagacgcg ggtgcccacg ggcgtcgtca ggatgtccgt aattgactgc
    10861 cggaggtggt caatgccctt cagctccttg ccactgtcac ggctcatgcc tcgggtcatt
    10921 agtcgcccgc tccggtatct tcactggctt cgatgattgc cgccccgcag ctgcagaggt
    10981 caccgatccg agcagtcggc ctctggttgg taaagaccgt gcgactgccg gtgatgatcg
    11041 tgttcaagcc atgcaggggg caggcgtgaa ggtcatcctt tcgggccacg gggcggctgt
    11101 tgacgaaggt gtcgtcgctc ccggtgatga tgatcccgcc gtgatcggtc acgtcgttta
    11161 gtcgagcgat gcctggcgtc gtagtcacgg gtttaggtca atacgacttg cggtcactgt
    11221 aacgttgccc tcggcggtca cgttaacgtc gccttgggct tcgacttgcg cctcctgcac
    11281 aaggatcaca atccgtcctt gggctgcggt gaggtcgatc ttgtactcat gcgcttcgcg
    11341 gtcgtactgg atgattgagt catcctcgaa ctgcgtcttt tggatcgttt ctttgtcctc
    11401 gatctggggg tagtcagtcg agaacgcgcc gggcatcgcg aagccctgac tgatctcgcc
    11461 ggagggggcc atcacgacga cggcctcacc gacctcgggc gcccaccaga accgatcctt
    11521 gcccgctcgc tgcgtgagcc acggaatcca gtcagtgagg agcagcgcct cgccgctctc
    11581 ctcgtcttcc tcgatcgcga cacggatcag ccccttggga tagtcagcct cggctaccct
    11641 gcctacgcgg agcaagttgc cgtgacgccg actgtctcga gtatctagtc gcgttgctgg
    11701 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    11761 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    11821 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    11881 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    11941 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    12001 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    12061 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    12121 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    12181 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    12241 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    12301 ctttgatctt ttctacgggg tctgacgctc agtctagttc agccagctcg tcgtgatgtc
    12361 gaaacccaag ccaccctcag aggtgaaggc cgcttcgagc acatcgggaa gcgtgtcgac
    12421 gacggtgctc ggtgcctcgg gtaagagcca gatagatgcg gtgacgttca ccgtcgcgat
    12481 cgtggcggga accacggtca ctgcgtccgt tacaactcgc acgtcgtcgg ccagcacgac
    12541 tgactcgacg gcttcgatta gcccgggaga ggcaaggcca tcaggctcgg tagacagaat
    12601 gctgattaac acctcgccgg gagcagggct gctcacagcc gcgtccttca cccgtgggtc
    12661 agccgtcagc gcttgatagc ggtaccaggc cgcaccgcct gcggtcgagc tgcccttgat
    12721 ccgctcgatg gtgcgatcgc gaagctcgcc atccggctca ttcggctgcc gcaccacgcc
    12781 gtagaacgcg gcaaggttgt cgaggtcggg gccgtttgag tagcgaagca gtgtcgcaag
    12841 aagtgcgtcg ttgatccgct gacgcaggat cagctcgcgg gctgcgcaga cctccagcag
    12901 cttgatgacc gggtcggatt cgaggatggc ggtgtagctg gcgtcacgcg atcgcaggtc
    12961 gtcgatcaag tcctgcagga tcagttcaaa gtccagcgct tcgatgatgg tgggcgcggg
    13021 aatcgtagca aagtcaagaa cggtcatgag acgactaagc cctccagcgt gatacgcctg
    13081 ccctcgggga tgtagtagcc gatcaggttc agctcgactt gaccagctgc gctggctgag
    13141 acgatgcgaa ccttctccag cttcagccgt ggctcccagc gatcgagcgc ttcagctgtg
    13201 gccgccacca ggtcaacgat gagggactgg ttgatcggtc tag
//
