LOCUS       sequence-273769-       12922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and 
            N2 are removed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 12922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz75_CB_operon_CsoS2_N3-N4
FEATURES             Location/Qualifiers
     source          1..12922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      144..171
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    193..212
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(227..232,233..250,251..257)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    265..281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        8374..8475
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8476..9135
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8542..8561)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        9200..9277
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9278..10360
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9297..9316)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      complement(11345..11933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11425..11444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      join(12888..12922,1..52)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
       61 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
      121 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
      181 actctagttt cgaattgtga gcgctcacaa tttcgaaacc ccaggcttga cactttatgc
      241 ttccggctcg tataatgtgt ggaattgtga gcggataaca atttcagcta ggtgcgcgaa
      301 tcccccatcc tttcaggagg aactcatggc agttaaaaag tatagtgctg gtgtaaaaga
      361 ataccggcag acctattgga tgccggaata cacaccgttg gattccgaca tccttgcatg
      421 cttcaaaatc accccacaac cgggtgttga tcgcgaagaa gccgcagccg cggttgcagc
      481 agaatcttca accggcacat ggaccaccgt gtggaccgat ttgctgaccg acatggacta
      541 ctacaaaggc cgtgcctatc gcattgaaga cgtacccggt gacgatgcgg cattctatgc
      601 ctttatcgcc tacccaatcg acctgttcga agaagggtca gttgttaacg tgtttacctc
      661 actggttggt aacgtgttcg gcttcaaagc ggtacgcggc ctgcgtctgg aagatgttcg
      721 cttcccactc gcctacgtta aaacctgtgg cggcccaccg cacggtattc aagtcgaacg
      781 cgacaagatg aacaaatatg gtcgcccact gttgggttgc accatcaagc caaaacttgg
      841 tttgtctgcg aaaaactacg gccgtgccgt atacgagtgc ctccgtggcg gcctcgactt
      901 cactaaagat gatgaaaaca tcaattctca gccgttcatg cgctggcgcg atcgcttctt
      961 gttcgtacaa gacgcgaccg aaactgctga agcccaaacc ggcgaacgca aaggccatta
     1021 cctcaacgta acggcgccaa ctcctgaaga aatgtacaag cgcgcagaat tcgccaaaga
     1081 aattggcgcg ccaatcatta tgcacgacta catcaccggt ggcttcacgg ccaacactgg
     1141 cttggccaag tggtgtcaag acaacggcgt actgctgcac atccaccgtg cgatgcatgc
     1201 ggttatcgac cgtaacccga accacggtat tcacttccgt gttctgacca agattctgcg
     1261 tttgtcgggt ggcgatcacc tgcacaccgg taccgttgtc ggcaaactgg aaggcgaccg
     1321 tgcctctact ctgggctgga ttgatttgct ccgcgaatcg tttatccctg aagatcgctc
     1381 gcgcggtatc ttcttcgatc aagactgggg ttcaatgcca ggcgtattcg ctgtggcctc
     1441 tggtggtatt cacgtatggc acatgcctgc gctggttaac atctttggtg acgactctgt
     1501 cctccaattc ggtggcggta cgctgggtca tccatggggc aacgctgccg gtgctgctgc
     1561 caaccgtgtt gctctggaag cctgcgtaga agcgcgtaac caaggccgcg atatcgaaaa
     1621 agaaggcaaa gaaattctga ctgctgctgc acagcacagc ccagaactga agattgccat
     1681 ggaaacttgg aaagagatca aattcgaatt tgacactgtc gacaaactcg acactcaaaa
     1741 tcgttgatcc ctcgtaccac acaacatact aaggtgagta accatggctg aaatgcagga
     1801 ttacaagcaa agcctcaaat atgagacttt ctcttatctt ccacccatga acgcggaacg
     1861 catccgcgct caaatcaagt acgcaattgc tcaaggctgg agccccggca ttgagcacgt
     1921 agaagtgaaa aactccatga accaatattg gtacatgtgg aaacttccct tcttcggcga
     1981 acaaaatgtc gacaacgtgt tggctgaaat tgaagcgtgt cgtagtgcgt atccaacaca
     2041 ccaggtcaaa ctggtggctt atgacaacta tgcgcaaagc ttaggtctgg ccttcgtggt
     2101 ctaccgcggc aactaagtca agtctgtcat tgcgctgtgc gtttctctac gcacagcact
     2161 tattcaagat ggggtcaaac atgaaagcgg ttaaaaccaa cggcaagccg tcgggtcgcg
     2221 ttcgaccaca gcaatcagtg gttcacaacg acgcaatgat cgaaaatgcc ggtgatacta
     2281 accaatcatc gtccacttca ttaaataatg aactttccga aatctgctcc atagcagacg
     2341 acatgccgga gcgttttggt tcacaagcca aaaccgtccg tgatatctgc cgtgcacgcc
     2401 gtcaagcgct ctctgagcgt ggaactcgcg ccgtgccgcc aaagccgcaa tctcaaggtg
     2461 gtccaggacg caatggctat caaattgatg gatacctaga taccgcactt catggccgcg
     2521 atgccgccaa gcgccaccgt gaaatgctct gtcaatacgg ccgcggcaca gcaccttcct
     2581 gcaagccaac aggccgtgtc aaaaattctg tacagtcggg caacgcagcg ccaaaaaagg
     2641 ttgaaaccgg tcacacccta tccggcggat ctgttacggg cacgcaagtg gatcgtaaat
     2701 ctcatgtgac tggaaacgag ccgggcactt gccgagcagt cacgggcacc gagtacgtag
     2761 gtactgagca attcacctct ttttgcaata ccagccccaa gccaaatgcg acgaaggtca
     2821 atgtgaccac aacggctcgt ggtcgccctg ttagcggtac ggaagtttca cggaccgaga
     2881 aggtaactgg caacgaatcc ggtgtctgcc gtaacgttac cggcaccgaa tacatgagta
     2941 atgaagctca cttttctcta tgtggaacag ccgcaaagcc ttcacaagcg gataaagtca
     3001 tgttcggcgc cacagcacga acgcatcaag tggtcagtgg cagtgatgaa ttcaggccct
     3061 cttctgttac gggtaacgaa tcgggtgcaa aacgcacaat taccggctcg cagtacgcag
     3121 acgaaggtct tgcgcgactc acgatcaacg gagcacctgc aaaagtagcc agaacccaca
     3181 cctttgcggg ctctgacgtt accggcacgg aaatcggtcg ctctactcgc gtaactggtg
     3241 atgaaagcgg ttcgtgtcgt tcaatctcag gcaccgagta tctcagtaac gagcaattcc
     3301 aatccttttg tgacacaaaa cctcaacgca gcccgttcaa ggttggccaa gatcgcacga
     3361 acaagggtca gtctgtgact ggtaacttgg ttgatcgttc cgaactggtt acaggtaacg
     3421 aaccaggttc atgctctcgg gttacaggct ctcagtatgg ccaaagcaaa atctgcggtg
     3481 gtggcgtggg aaaagtgcgc tcaatgcgca cccttcgcgg cacctcagta tctggccaac
     3541 agctagatca tgccccaaag atgtccggtg acgagcgcgg cgggtgcatg cccgtcaccg
     3601 gtaatgagta ctacggtcgt gaacatttcg aaccgttttg tacgagcacc ccagagcccg
     3661 aagctcaatc aactgaacaa tcattgacct gtgaaggaca aattattagc ggcacttcag
     3721 ttgacgccag tgatttggtc acaggaaatg aaatcggtga acagcaactc atcagcggtg
     3781 acgcctatgt tggcgcgcag cagacaggtt gccttcccac tagtccacgc ttcaaccaaa
     3841 ctggcaatgt tcagtcaatg ggttttaaga acaccaatca gccagaacaa aactttgcac
     3901 caggtgaagt aatgcctact gactttagta ttcaaacccc agctcgctcg gctcagaatc
     3961 gcattacagg taacgacatt gcgccctcag gtcgcattac aggccctggt atgctggcaa
     4021 ccggcttgat tacaggaacc cccgaattca ggcacgctgc gcgcgagttg gttggttctc
     4081 cacaacccat ggcaatggcc atggccaacc gtaataaggc tgctcaagca cctgttgtgc
     4141 agccagaagt ggttgcaact caggaaaagc ctgagttggt atgtgcacca agaagcgatc
     4201 aaatggatcg tgtgagtggc gaaggcaaag aacgttgcca catcactggc gatgactggt
     4261 cagttaacaa gcacatcacc ggtacagccg gtcaatgggc gagtggtcgc aacccttcca
     4321 tgcgcggtaa tgcgcgtgtg gtcgaaacca gcgcgtttgc caatcgcaat gtgccaaaac
     4381 ctgaaaagcc gggctccaag atcacgggca gtagtggtaa tgacacccaa ggtagtctga
     4441 tcacttactc cggcggcgcg cgcggttgag cgttaagtaa agtgtaacga tcatgaacac
     4501 ccgtaacaca cgaagcaagc aacgcgcacc gtttggtgtt agctcatcag tcaaacctcg
     4561 gcttgacttg attgagcaag caccaaaccc tgcctatgac cgccatcctg cttgcataac
     4621 gttgcctgag cgtacctgcc ggcacccgtt aactgacctt gaagccaacg aacaactggg
     4681 tcgttgcgag gatagcgtca agaaccgttt tgatcgcgtt atccctttct tgcaagttgt
     4741 tgctggcatt cctcttggtc tggattatgt tacccgtgtt caagaattag cccagtcgtc
     4801 gcttggacat acgctgcccg aagaattact caaagataat tggatcagtg ggcacaattt
     4861 aaaaggcatt tttggctacg cgactgctaa agcactaaca gcagccacgg aacaattcag
     4921 tcgtaaaata atgtctgaga aggacgattc cgcatcggcc attggcttct tcctggattg
     4981 cgggttccac gcagtggaca taagcccttg tgccgatggt cgtctcaaag ggttactgcc
     5041 ttacatattg cgtttgcccc ttacggcatt cacctatcgt aaagcctacg caggctcgat
     5101 gttcgatatt gaagatgatc tggcacagtg ggagaaaaat gaactccgcc gttatcgtga
     5161 aggtgttcca aatacagcgg atcagccaac acgatacctg aaaattgctg tgtatcattt
     5221 cagcacctct gacccgacac actctggctg cgcggcacac ggcagtaatg atcgtgcagc
     5281 actggaagcg gctttaaccc agctgatgaa attcagagaa gcggttgaaa atgcccattg
     5341 ctgcggcgca agtatcgata ttttactgat tggcgttgat acggatacgg atgccattcg
     5401 cgttcatatt ccggatagca aaggtttttt gaatccgtat cgttatgttg acaacacagt
     5461 aacttatgcg caaacactac atctggcgcc ggatgaggct cgtgtgatta ttcacgaagc
     5521 aattctcaac gcaaaccgca gcgatggttg ggctaaagga aatggagtag ccagcgaggg
     5581 gatgcgtcgt tttattggtc agcttttgat caacaacctc tcgcaaatcg attacgtagt
     5641 aaatcgtcat ggtggtcgct atccacccaa tgatattggt catgctgagc gatatatcag
     5701 tgttggtgat ggttttgatg aagttcaaat ccggaattta gcctactacg cgcatttgga
     5761 tacggttgaa gaaaatgcga ttgatgtgga tgtgggaatc aaaattttca ccaaacttaa
     5821 tttgagtcga ggtttaccga ttccgattgc catccactat cgctatgacc ccaatgttcc
     5881 aggctccaga gaaagaaccg tggtcaaagc aagacggata tacaacgcca ttaaagagcg
     5941 gttctcatcc ttggatgagc agaatctatt gcagtttcgt ttgagcgttc aggcgcagga
     6001 tatcggaagc ccgattgaag aggttgcatc cgcatgaaaa tcatgcaagt tgagaaaacg
     6061 ttggtttcaa caaaccgtat tgctgatatg ggtcacaaac cactattagt ggtatgggag
     6121 aaaccgggcg cgcccaggca ggtcgccgtg gatgcgattg gctgcatacc gggcgattgg
     6181 gttttgtgcg ttgggtcatc ggcagcacga gaggctgcag gaagcaagtc ttatccctct
     6241 gatttgacga ttatcgggat tattgatcag tggaatggtg agtaatggaa gtaatgcgcg
     6301 ttcgttccga cctaatcgca acacgcagga ttcccggtct taaaaatatc tctttgcgtg
     6361 tcatggagga tgctacgggt aaggtcagtg tcgcttgcga tcccattggc gttcccgagg
     6421 gatgttgggt ctttacgatt agcggctctg ccgctcggtt tggcgtgggt gattttgaga
     6481 ttctcacgga tttgacgatt ggtggcatca tcgatcactg ggtaacttga ccatcgctag
     6541 atgagttgat tttgaatgag tctttattga ggagagaaga aatggcagca gtaacaggta
     6601 ttgcactggg tatgattgaa acacgtggtc tggttccagc gattgaagct gccgatgcca
     6661 tgaccaaggc cgccgaagta cgtttggttg gccgtcaatt tgttggtggt ggttacgtga
     6721 ccgttttggt ccgtggtgaa accggtgccg tcaacgcagc agttcgtgcg ggcgctgatg
     6781 cctgcgaacg agtcggcgat ggtctggtcg cggcgcatat cattgcccgt gtccattccg
     6841 aagtcgaaaa catcctgccg aaagcccctg aagcttaagg attgggaaag acgaaccggc
     6901 gcaggcttgt tccggttctt tgcataaagt gacagcttag gagtttattt aaatggctga
     6961 tgtaactggt attgctctgg gtatgatcga aacacgtggc ttagttcctg cgattgaagc
     7021 agcggacgcc atgactaaag cggctgaagt gcgtttggtc ggtcgtcaat ttgttggtgg
     7081 cggttacgtc accgtattgg ttcggggcga aacaggcgct gttaacgccg ctgttcgtgc
     7141 tggcgccgat gcttgcgaac gtgttggtga tggcttggtt gctgcgcaca tcattgcgcg
     7201 tgtccactca gaagtagaaa acattctgcc taaggcgcca caagcctaag tcagatattc
     7261 ctaagacggc tcacttcggc gacacccgtc gccgaaaccg tctaaccaaa tttctgggct
     7321 cgttctgaac gtgctcaaca ctagttttag aggatctgtt atggcaacga ctcacggtat
     7381 tgccctgggc atgattgaaa cacgaggatt ggttcctgcc attgaagccg cagatgccat
     7441 gaccaaagcg gcggaagtcc gtctggtcgg acgatcattt gttggcggcg gttacgtgac
     7501 cgtaatggtt cgtggtgaga caggtgcagt aaatgctgcc gttcgtgcgg gtgctgacgc
     7561 ctgtgaacgt gttggcgatg gcctggttgc tgcgcacatc attgcgcgcg ttcattctga
     7621 agttgagatc atcctacccg agacgcccga agactcagat tccgcgtggt gtatcgcaaa
     7681 tctgaatagc taatgtctag tagggaagat gcgcatgaac aacattgatt tgcgcgtcta
     7741 ttcgtttatc gattcgcttc aaccgcagct tgcatcctat cttgcgacat catcgcaagg
     7801 ctttcttccc gttccgggtg atgcctgctt gtggattgaa gtcgcgccgg gcatggccgt
     7861 ccatcgcctc agtgatattg cgctaaaggc cacgaacgtt cgtctcggcg aacaggtagt
     7921 cgagcgtgct tttggctcga tggaaattca ttaccgaaac caaagcgacg ttctcgcatc
     7981 cggtgaggcc gttttaagag aaatcaatca cgcgcaagaa gatcgtctgc cttgtcgcat
     8041 cgcatggaaa gagatcattc gagcgattac ccccgatcat gccaccttga tcaatcgcca
     8101 gttgcgtaaa ggctctatgc tgttgccggg caaaagcatg ttcatccttg aaacagaacc
     8161 ggcaggttat attgttcagg ctgccaacga ggccgagaaa gcagctcatg tgactctgat
     8221 cgatgtacgt gcttttggta actttggtcg cctgaccatg atgggcagcg aagcggaaac
     8281 cgaagaagcc atgcgggcgg ctgaggccac aatcgcaagc atcaacgcgc gtgcgcgtcg
     8341 cgctgaaggg ttctaagcgg ccgcgtagga tccgatcggc acgtaagagg ttccaacttt
     8401 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
     8461 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     8521 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     8581 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     8641 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg
     8701 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     8761 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     8821 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     8881 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     8941 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac tatgggcaaa
     9001 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     9061 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     9121 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttgaattcc tactagtcga
     9181 agcggcatgc atttacgttg acaccatcga atggtgcaaa acctttcgcg gtatggcatg
     9241 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
     9301 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
     9361 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     9421 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     9481 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     9541 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     9601 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     9661 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     9721 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     9781 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     9841 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     9901 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     9961 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
    10021 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
    10081 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
    10141 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
    10201 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
    10261 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10321 gctggcacga caggtttccc gactggaaag cgggcagtga tcccacagcc gccagttccg
    10381 ctggcggcat tttaactttc tttaatgaat ctagtgacaa gccggggcag acgtgagccg
    10441 tagtcccgtc gccagacgcg ggtgcccacg ggcgtcgtca ggatgtccgt aattgactgc
    10501 cggaggtggt caatgccctt cagctccttg ccactgtcac ggctcatgcc tcgggtcatt
    10561 agtcgcccgc tccggtatct tcactggctt cgatgattgc cgccccgcag ctgcagaggt
    10621 caccgatccg agcagtcggc ctctggttgg taaagaccgt gcgactgccg gtgatgatcg
    10681 tgttcaagcc atgcaggggg caggcgtgaa ggtcatcctt tcgggccacg gggcggctgt
    10741 tgacgaaggt gtcgtcgctc ccggtgatga tgatcccgcc gtgatcggtc acgtcgttta
    10801 gtcgagcgat gcctggcgtc gtagtcacgg gtttaggtca atacgacttg cggtcactgt
    10861 aacgttgccc tcggcggtca cgttaacgtc gccttgggct tcgacttgcg cctcctgcac
    10921 aaggatcaca atccgtcctt gggctgcggt gaggtcgatc ttgtactcat gcgcttcgcg
    10981 gtcgtactgg atgattgagt catcctcgaa ctgcgtcttt tggatcgttt ctttgtcctc
    11041 gatctggggg tagtcagtcg agaacgcgcc gggcatcgcg aagccctgac tgatctcgcc
    11101 ggagggggcc atcacgacga cggcctcacc gacctcgggc gcccaccaga accgatcctt
    11161 gcccgctcgc tgcgtgagcc acggaatcca gtcagtgagg agcagcgcct cgccgctctc
    11221 ctcgtcttcc tcgatcgcga cacggatcag ccccttggga tagtcagcct cggctaccct
    11281 gcctacgcgg agcaagttgc cgtgacgccg actgtctcga gtatctagtc gcgttgctgg
    11341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    11401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    11461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    11521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    11581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    11641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    11701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    11761 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    11821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11941 ctttgatctt ttctacgggg tctgacgctc agtctagttc agccagctcg tcgtgatgtc
    12001 gaaacccaag ccaccctcag aggtgaaggc cgcttcgagc acatcgggaa gcgtgtcgac
    12061 gacggtgctc ggtgcctcgg gtaagagcca gatagatgcg gtgacgttca ccgtcgcgat
    12121 cgtggcggga accacggtca ctgcgtccgt tacaactcgc acgtcgtcgg ccagcacgac
    12181 tgactcgacg gcttcgatta gcccgggaga ggcaaggcca tcaggctcgg tagacagaat
    12241 gctgattaac acctcgccgg gagcagggct gctcacagcc gcgtccttca cccgtgggtc
    12301 agccgtcagc gcttgatagc ggtaccaggc cgcaccgcct gcggtcgagc tgcccttgat
    12361 ccgctcgatg gtgcgatcgc gaagctcgcc atccggctca ttcggctgcc gcaccacgcc
    12421 gtagaacgcg gcaaggttgt cgaggtcggg gccgtttgag tagcgaagca gtgtcgcaag
    12481 aagtgcgtcg ttgatccgct gacgcaggat cagctcgcgg gctgcgcaga cctccagcag
    12541 cttgatgacc gggtcggatt cgaggatggc ggtgtagctg gcgtcacgcg atcgcaggtc
    12601 gtcgatcaag tcctgcagga tcagttcaaa gtccagcgct tcgatgatgg tgggcgcggg
    12661 aatcgtagca aagtcaagaa cggtcatgag acgactaagc cctccagcgt gatacgcctg
    12721 ccctcgggga tgtagtagcc gatcaggttc agctcgactt gaccagctgc gctggctgag
    12781 acgatgcgaa ccttctccag cttcagccgt ggctcccagc gatcgagcgc ttcagctgtg
    12841 gccgccacca ggtcaacgat gagggactgg ttgatcggtc tagtcatcaa ataaaacgaa
    12901 aggctcagtc gaaagactgg gc
//
