LOCUS       sequence-27378-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Dann CT, Alvarado AL, Molyneux LA, Denard BS, Garbers DL, Porteus MH
  TITLE     Spermatogonial stem cell self-renewal requires OCT4, a factor 
            downregulated during retinoic acid-induced differentiation.
  JOURNAL   Stem Cells. 2008 Nov . 26(11):2928-37.
  PUBMED    18719224
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2008 Nov . 26(11):2928-37."
COMMENT     Sequence Label: pLLU2G-Oct3
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     149..169
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     300..316
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     301..317
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(359..392)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata
       61 atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc tataagagat
      121 acaaatacta aattattatt ttaaaaaaca gcacaaaagg aaactcaccc taactgtaaa
      181 gtaattgtgt gttttgagac tataaatatc ccttggagaa aagccttgtt tgaaccgtgt
      241 gaggtggaac ttcaagagag ttccacctca cacggttctt ttttaacgcg cggtgaccct
      301 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat
      361 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggcccg
      421 cgggcccggg atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
      481 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
      541 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
      601 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
      661 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
      721 cggtgaacgc cgatgattat ataggacgcg ccgggngtgg cacagctagt tccgtcgcag
      781 ccgggattgg ncgc
//
