LOCUS       sequence-273781-        7534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MosSCI backbone for MosSCI-SapTrap assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Fan X, Henau S, Feinstein J, Miller SI, Han B, Frokjaer-Jensen C, 
            Griffin EE
  TITLE     SapTrap Assembly of Caenorhabditis elegans MosSCI Transgene Vectors.
  JOURNAL   G3 (Bethesda). 2019 Dec 17. pii: g3.119.400822. doi: 
            10.1534/g3.119.400822.
  PUBMED    31848219
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2019 Dec 17. pii: g3.119.400822. doi: 
            10.1534/g3.119.400822."
COMMENT     Sequence Label: pXF87
FEATURES             Location/Qualifiers
     source          1..7534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(199..303)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     371..389
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(427..449)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    772..792
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2130..2154
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2300..2311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4251..4268
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4252..4268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4481..4497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4481..4497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4504..4528)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5956..5976)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(6113..6701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6193..6212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6872..7534,1..129,130..198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7495..7514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
       61 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      301 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
      361 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
      421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
      481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
      541 taactatgcg gcatcagagc agattgcact gagagtgcac catatgcggt gtgaaatacc
      601 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
      661 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      721 atgtgctgca aggcgattaa gttgggtaac gccagggatc cgccaagcta tcaactttgt
      781 atagaaaagt tgcttgtcgg attatgggca cttcttttat cctctacttt ctttctagaa
      841 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
      901 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
      961 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     1021 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     1081 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     1141 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     1201 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     1261 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     1321 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     1381 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     1441 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     1501 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     1561 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     1621 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     1681 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     1741 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     1801 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     1861 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     1921 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     1981 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     2041 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     2101 gattccatga tggtagcaaa ctcacttcgt caagtttgta caaaaaagca ggcttgagcc
     2161 aatttatcca agtccttgta aaaagtattc gaaaattgtt aacggataaa atgtttatta
     2221 taatatcaaa aacaatttgt cagttgacca ctttttgata tatttgacag aaaccgggat
     2281 gaattggctc aaagttaggg cgcccttcta ttacagggtt tctgataaca aaccggttat
     2341 taactcccaa caagggatga tttcaattca tcatgctcaa attgacccaa attaagttac
     2401 atgacaaatt catcgccctt ttcaactctt cttggctcat catctgttat tctgttccta
     2461 ttctgtctgc accctatacc cctttgcata ctctctcgtc attcctttct tggatagtgt
     2521 cttcttctcc ccagctctgc tacttctatg acttgcgccc gctgcttttc cgctcgttcc
     2581 tctcctctct gacgtcttct tctgcttctt ttctccttcc tttttccctt ctcaatcttc
     2641 ctctttttcc catttctgtc aatcattcga agaagaagaa gaagaagacc ctcattcatt
     2701 tcattttttt ttctgtcggt gtgtgctgct cggttaagag tgagctctct attccacgtc
     2761 ttcttctttt tcttcttcga ttcgaatcaa tcactccacc aaaacgcatt cgtttttggg
     2821 attcaccccg cggttcgcaa taggtttctt tttcaaatta ttagcgttat aaatagaaaa
     2881 atggtggagt ttcaataaaa atgataattt acaaagtgta ttttgattac atgtactcaa
     2941 aaaggttgaa atttttcata ccagttttcc ggaaatccat ctgatatcat tatcgtattt
     3001 ttctttttaa aaatgttttc aaaaaaaaac aaaatatagc tggttatttg gcaccctcta
     3061 attaccattt tcctgtcaca ccacactctt ttccttcttc cacttctttc cgttttcagc
     3121 cgcttccaac caaaccgata tgaaagccga gcaacaacga tcgattccac ccggctcggc
     3181 gaccttcccg tcgcaggtga gactcagaaa actagagaaa ccgctcaact aactcttgat
     3241 atccgatttc attcttttct cttttctttt tgttgaactt ttccatattt ccagatgcca
     3301 cggccaccac caagcaccga acaaggaatc acaacggaat cggagcttgc gaagaaagct
     3361 caaatcactc cgaacgacgt tttagcactt ccgggaatca ctcaaggtat cttgctccct
     3421 ttggatttca gtttaaaaca taaatttagg attcttatgc tccccatctg cgaacatcta
     3481 taacatcgag ttcaccaagt tccaaatccg tgatctggac actgagcaag tgcttttcga
     3541 gatcgccaaa ccggagaacg atcaggagaa tgatgagtcg ccacaggagt cggcaagata
     3601 cgtgcgttat agatttgctc caaacttttt gaaactcaaa acggtcggag caactgtgga
     3661 attcaaagta ggagacatcc caatccatca tttccgaatg atcgaacgtc acttcttcaa
     3721 agatcgcctt ctgaagtgtt ttgactttga attcggattc tgtattccga attcacgaaa
     3781 caactgtgaa catatctatg agttccctca actctctcaa caactcagtg agtcattatt
     3841 ctaaaaagta caattcaaaa gactaatctc tttttcagtg gacgacatga tcaacaatcc
     3901 aaacgagact cgttctgaca gcttctattt cgtcgataac aaactcgtca tgcacaacaa
     3961 agccgactac tcatatgatg cataaatatt taatacaaaa atgttctgga taattattct
     4021 gtcgaataga aaaaaaactc caaatgtgat taaattccaa taattcctgt ctagtttgtt
     4081 ccttcccctt ccccttctca tgttcaatgc attcctaagc ttttcagttc cccccttgtt
     4141 tctatatttt ttcgctgtcc tgtcacactc gctaaaaaca ctaatcacac ggaaatctgt
     4201 tttcaataaa aactccaact ttaactcatt ttcaatttca actgcacggg tgtaaaacga
     4261 cggccagtcc gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta
     4321 ggtatctaga accggtgacg tcaccatggg aagcttcgtg gatccagata tcctgcagga
     4381 attcctcgag tggcgaagag cccatggatc ccatatggaa ttctgcaggc ctgctcttcg
     4441 gtaactagtg agtcgtatta cgtagcttgg cgtaatcatg gtcatagctg tttcctgccc
     4501 gtgacccagc tttcttgtac aaagtggtaa gtgcaagtaa gatcagtgtt tgtttcgata
     4561 tcagtctgtt tcgtaacggt cttctgtata actacaaaaa agaataaaaa accgtatcac
     4621 aactgagaaa aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt attttgtaaa
     4681 aaatcgagtt tttctccaaa ataacgcacg caatcaaaaa caagaaaact aacgtgttct
     4741 cctgggcgtg gaactcctta tcaagattac cattattgtc tgtcagaatt gatatttcaa
     4801 ttatttcata tctagacaaa cccgggtgtc ttgcatttat tagtactgtg agtttacata
     4861 caaacgaggc ttactcagat aacatagagt cacctctaat tagcaaaaac ccatttctaa
     4921 ttagctattt cgaccagttc taaagtcacc gtaattagaa atggtttttg tagcttttct
     4981 aattagaaat gaccccgaga taagtgaaat atggcatttt ctgggttttc tagtaagaaa
     5041 tgatcccgca cgtagtgatt ccgcgttaca agccaaattc aaattaaaaa agtatattac
     5101 acaattcaag ttacattatt ttcaggaaac aatggcgaag tgcaaagcag agattcaaaa
     5161 ggaggaaaga gacaacagat atgaacgaag acttcttgaa aagagaaaga gacatgaggt
     5221 gagtaaccat gataataaat aatcaagtaa gggctataga tttataaatt ctggagttaa
     5281 cagtttcgaa aaaaagtagt tttcatatgt gcgaaggaat atcaaaaata ggggaatagc
     5341 ctaaacgagt gagaaaatta aatgtttgtt attttttcaa aaatgtctgc agggccgtta
     5401 tcctttcaaa tgcactctat caaacataga tcatatccat catatcttat ccagaattta
     5461 tatcatatcc aagattgaga gcgtttcaag gaaaatctca aacaaccata caattttgta
     5521 gatcgacaac aaggcctcca cttcttcatc gatggcctct gcagcacctg caccaccaag
     5581 tttgtatttt tcatattaat ataaagtgag ccacgcatag tcgtttttag gatttttgcc
     5641 agatcgatgt cgcgcaaaca gactgttttt ttttctttga aagccgtgcg tgacatgggc
     5701 cacggattgt aacgccgcgc tggatggata aaaatttatt cagtttttta gcctttacaa
     5761 aatatggttc gactactttc tttattttta catttttata ggtgctcatt gagctgagat
     5821 attttgggta taatcggaaa atcgctgatt ttggtcattt ttctgcacta aagattacac
     5881 aaaaagttca gtatttagat tcattgttga aaattgggga tgatctacag atacatatcg
     5941 ccgtgttcaa aaacacaact ttattataca tagttgataa ttcgatggta tcagctcact
     6001 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6061 caaaaggcca gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6121 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6181 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6241 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6301 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6361 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6421 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6481 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6541 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6601 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6661 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6721 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6781 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6841 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6901 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6961 ctacgatacg ggagggctta ccatctgacc ccagtgctgc aatgataccg cgagacccac
     7021 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7081 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7141 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7201 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7261 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7321 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7381 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7441 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7501 ccgcgccaca tagcagaact ttaaaagtgc tcat
//
