LOCUS       sequence-27379-e         291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Dann CT, Alvarado AL, Molyneux LA, Denard BS, Garbers DL, Porteus MH
  TITLE     Spermatogonial stem cell self-renewal requires OCT4, a factor 
            downregulated during retinoic acid-induced differentiation.
  JOURNAL   Stem Cells. 2008 Nov . 26(11):2928-37.
  PUBMED    18719224
REFERENCE   2  (bases 1 to 291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2008 Nov . 26(11):2928-37."
COMMENT     Sequence Label: pLLU2G-Oct3
FEATURES             Location/Qualifiers
     source          1..291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    161..194
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(236..252)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(237..253)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gccgtctnac gtcgggcgga ggatcaggac gctcgctgcg cccttcgtct gacgtggcag
       61 cgctcgccgn tgaggagggg ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag
      121 atcccgggcc cgcgggccgc ttaagcttgg aacccttaat ataacttcgt ataatgtatg
      181 ctatacgaag ttattaggtc cctcgacctg ctggaatctc gtgaagcgag cttatcgata
      241 ccgtcgacct cgagggtcac cgcgcgttaa aaaagaaccg tgtgaggtgn a
//
