LOCUS       sequence-273793-       11628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C,
            Fritz-Laylin LK, Buchler NE
  TITLE     Genetic transformation of Spizellomyces punctatus, a resource for 
            studying chytrid biology and evolutionary cell biology.
  JOURNAL   Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.
  PUBMED    32392127
REFERENCE   2  (bases 1 to 11628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-11"; volume: "9. pii: 52741. doi"; pages: " 
            10.7554/eLife.52741"
COMMENT     Sequence Label: pGI3EM29
FEATURES             Location/Qualifiers
     source          1..11628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             197..1270
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1336..1530
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    1874..2014
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2029..2046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2200..2788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2280..2299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2883..3698)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2883..3698)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3606..3625
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3690..3709
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    3991..4015
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    4192..4213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4228..4233,4234..4251,4252..4258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4266..4282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4271..4293
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4290..4306
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4290..4306
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(4321..4508)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     4429..4453
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(4524..5234,5235..5237,5238..5240))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4566..4587)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4915..4934
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5174..5195
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5256..6281)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             complement(5256..6281)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(6580..6597)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6580..6596)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6589..6611)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6792..6816
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6983..7786)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7787..8002)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8205..8223)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      8264..9606
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    8596..8643
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             10767..11396
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
       61 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
      121 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
      181 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
      241 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
      301 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
      361 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
      421 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
      481 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
      541 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
      601 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
      661 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
      721 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
      781 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
      841 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
      901 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
      961 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     1021 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     1081 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     1141 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     1201 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     1261 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     1321 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     1381 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     1441 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     1501 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     1561 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     1621 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     1681 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     1741 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     1801 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     1861 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     1921 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     1981 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     2041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2641 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2821 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcaggatc atgaattaat
     2881 tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     2941 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     3001 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     3061 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     3121 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     3181 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     3241 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     3301 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     3361 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
     3421 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     3481 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     3541 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     3601 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     3661 ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     3721 atgtaagcag acagttttat tgttcatgat ctggatcaca ggcagcaacg ctctgtcatc
     3781 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
     3841 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
     3901 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
     3961 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
     4021 gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga
     4081 attgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4141 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4201 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4261 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4321 ccggtagagg tgtggtcaat aagagcgacc tcatactata cctgagaaag caacctgacc
     4381 tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa
     4441 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag
     4501 aaattcgctt ataagtggcg cgccttgtac agctcgtcca tgccgagagt gatcccggcg
     4561 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc
     4621 ttggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     4681 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     4741 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     4801 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     4861 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     4921 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     4981 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca catcaggccg
     5041 tagccgaagg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     5101 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     5161 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     5221 tcctcgccct tgctcaccat ggatcctcct cctccttcct ttgccctcgg acgagtgctg
     5281 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
     5341 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
     5401 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
     5461 tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga
     5521 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     5581 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     5641 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     5701 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     5761 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     5821 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     5881 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc
     5941 tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     6001 cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc
     6061 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     6121 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     6181 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     6241 ttctcgacag acgtcgcggt gagttcaggc tttttaccca ttaattaatt tgtgtgtgtg
     6301 atggatgaga gtggtgcggg tgaaaggtct tggtcaagtc gcaaagtgtt tgtgaaaaag
     6361 acgtagcggg ggaagcgcac acacgcccgc cttttataga cgaagcttcg agcaggtcat
     6421 ttaacgatgc ttagtagcaa tatacaataa agcctaattg ggactaatta cggtcacgtg
     6481 aaaaactcac catgtagcct tttatggaaa tattgtacac gctactaagc cactgtaata
     6541 tcggcctcat cgtctccgct tggagcataa aagcttggca ctggccgtcg ttttacaacg
     6601 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6661 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6721 cctgaatggc gaatgagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa
     6781 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     6841 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     6901 tgccaggtaa taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg
     6961 catttactta taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc
     7021 agcctgcttt tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc
     7081 ctcttccaac aataataatg tcagatcctg tagagaccac atcatccacg gttctatact
     7141 gttgacccaa tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat
     7201 cgtaaccttc atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt
     7261 tgtcgctctt cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct
     7321 tgcatgacaa ttctgctagc atcaaaaggc ctctaggttc ctttgttact tcttctgccg
     7381 cctgcttcaa accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg
     7441 cccattctgc tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt
     7501 cagcaaattt tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg
     7561 gcttaactgt gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac
     7621 aaattttggg acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca
     7681 atgaagcaca caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag
     7741 gactaggatg agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt
     7801 tcctgcaggt ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt
     7861 caacactaca tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct
     7921 tctgttcgga gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat
     7981 aaaaaaaaaa tgatgaattg aaaaggtggt atggtgcact ctcagtacaa tctgctctga
     8041 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8101 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8161 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     8221 atttttatag gttaatgtca tgataataat ggtttcttag tatgatccaa tatcaaagga
     8281 aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct
     8341 aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga
     8401 ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata
     8461 aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
     8521 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
     8581 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     8641 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
     8701 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
     8761 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
     8821 ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta
     8881 ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata
     8941 cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat
     9001 aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca
     9061 atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag
     9121 cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa
     9181 tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag
     9241 agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa
     9301 aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt
     9361 aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
     9421 gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
     9481 ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     9541 tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
     9601 ttcgttcagg tggcactttt ccttctgacg ttcagtgcag ccgtcttctg aaaacgacat
     9661 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     9721 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     9781 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     9841 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     9901 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     9961 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
    10021 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
    10081 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
    10141 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
    10201 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
    10261 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
    10321 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
    10381 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
    10441 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
    10501 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
    10561 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
    10621 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
    10681 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
    10741 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
    10801 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
    10861 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
    10921 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
    10981 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
    11041 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
    11101 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
    11161 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
    11221 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
    11281 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
    11341 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
    11401 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
    11461 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
    11521 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
    11581 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccaga
//
