LOCUS       sequence-27381-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Dann CT, Alvarado AL, Molyneux LA, Denard BS, Garbers DL, Porteus MH
  TITLE     Spermatogonial stem cell self-renewal requires OCT4, a factor 
            downregulated during retinoic acid-induced differentiation.
  JOURNAL   Stem Cells. 2008 Nov . 26(11):2928-37.
  PUBMED    18719224
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2008 Nov . 26(11):2928-37."
COMMENT     Sequence Label: pLLNeo
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(129..146)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    135..359
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(416..728)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(477..497)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
       61 catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatcaattct ctagagctcg
      121 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      181 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      241 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
      301 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
      361 ttctgaggcg gaaagaacca gctgggggcg cgcccctcga gggtcaccgc gcgttaacaa
      421 ggcttttctc caagggatat ttatagtctc aaaacacaca attactttac agttagggtg
      481 agtttccttt tgtgctgttt tttaaaataa taatttagta tttgtatctc ttatagaaat
      541 ccaagcctat catgtaaaat gtagctagta ttaaaaagaa cagattatct gtcttttatc
      601 gcacattaag cctctatagt tactaggaaa tattatatgc aaattaaccg gggcagggga
      661 gtagccgagc ttctcccaca agtctgtgcg agggggccgg cgcgggccta gagatggcgc
      721 gtcggatctc tagagcgggc ccggtactaa ccaaactgga tctctgctgt ccctgtaata
      781 aacccgaaaa ttttg
//
