LOCUS       sequence-273880-        9004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag CDK7
FEATURES             Location/Qualifiers
     source          1..9004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    805..1004
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1205..1621
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1551..1573
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1589..1605
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..1686
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1741..1765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2804..2828)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2887..3348
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2943..2960)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3170..3189
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3353..4147
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3407..3426)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4017..4036
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4334..4803
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4839..4943
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4944..5012,5013..5804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5162..5181)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7570..8158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8059..8078
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8312..8329
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8433..8860)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8543..8564)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8755..8774
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgcg
      601 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
      661 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
      721 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
      781 accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg
      841 tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg
      901 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
      961 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     1021 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     1081 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     1141 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1201 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1261 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1321 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1381 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1441 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1501 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1561 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1621 cggccggatc cagaattcgc caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
     1681 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
     1741 acaagtttgt acaaaaaagt tggcatggct ctggacgtga agtctcgggc aaagcgttat
     1801 gagaagctgg acttccttgg ggagggacag tttgccaccg tttacaaggc cagagataag
     1861 aacaccaacc aaattgtcgc cattaagaaa atcaaacttg gacatagatc agaagctaaa
     1921 gatggtataa atagaaccgc cttaagagag ataaaattat tacaggagct aagtcatcca
     1981 aatataattg gtctccttga tgcttttgga cataaatcta atattagcct tgtctttgat
     2041 tttatggaaa ctgatctaga ggttataata aaggataata gtcttgtgct gacaccatca
     2101 cacatcaaag cctacatgtt gatgactctt caaggattag aatatttaca tcaacattgg
     2161 atcctacata gggatctgaa accaaacaac ttgttgctag atgaaaatgg agttctaaaa
     2221 ctggcagatt ttggcctggc caaatctttt gggagcccca atagagctta tacacatcag
     2281 gttgtaacca ggtggtatcg ggcccccgag ttactatttg gagctaggat gtatggtgta
     2341 ggtgtggaca tgtgggctgt tggctgtata ttagcagagt tacttctaag ggttcctttt
     2401 ttgccaggag attcagacct tgatcagcta acaagaatat ttgaaacttt gggcacacca
     2461 actgaggaac agtggccgga catgtgtagt cttccagatt atgtgacatt taagagtttc
     2521 cctggaatac ctttgcatca catcttcagt gcagcaggag acgacttact agatctcata
     2581 caaggcttat tcttatttaa tccatgtgct cgaattacgg ccacacaggc actgaaaatg
     2641 aagtatttca gtaatcggcc agggccaaca cctggatgtc agctgccaag accaaactgt
     2701 ccagtggaaa ccttaaagga gcaatcaaat ccagctttgg caataaaaag gaaaagaaca
     2761 gaggccttag aacaaggagg attgcccaag aaactaattt tttacccaac tttcttgtac
     2821 aaagtggttt gatctagtcg accaattccg gttattttcc accatattgc cgtcttttgg
     2881 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     2941 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3001 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     3061 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     3121 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     3181 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     3241 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc
     3301 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata ccatgattga
     3361 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3421 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3481 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     3541 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     3601 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     3661 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     3721 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     3781 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     3841 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     3901 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     3961 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4021 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4081 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4141 cttctgagcg ggactctggg gttcgcatcg ataaaataaa agattttatt tagtctccag
     4201 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     4261 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
     4321 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     4381 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     4441 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
     4501 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     4561 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     4621 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
     4681 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     4741 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     4801 tcacaggcca gtggggccgc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4861 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4921 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4981 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5041 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5101 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5161 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5221 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5281 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5341 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5401 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5461 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5521 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5581 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5641 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5701 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5761 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5821 actcatatat actttagatt gatttgcggc cgcgaaaagg acaagcagcg aaaattcacg
     5881 cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc
     5941 atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc
     6001 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     6061 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
     6121 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
     6181 atatgctatc cagatatttg ggtagtatat gctacccatg gcaacattag cccaccgtgc
     6241 tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg cttggcgctc aggcgcaagt
     6301 gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct atcttggccc gcccacctac
     6361 ttatgcaggt attccccggg gtgccattag tggttttgtg ggcaagtggt ttgaccgcag
     6421 tggttagcgg ggttacaatc agccaagtta ttacaccctt attttacagt ccaaaaccgc
     6481 agggcggcgt gtgggggctg acgcgtgccc ccactccaca atttcaaaaa aaagagtggc
     6541 cacttgtctt tgtttatggg ccccattggc gtggagcccc gtttaatttt cgggggtgtt
     6601 agagacaacc agtggagtcc gctgctgtcg gcgtccactc tctttcccct tgttacaaat
     6661 agagtgtaac aacatggttc acctgtcttg gtccctgcct gggacacatc ttaataaccc
     6721 cagtatcata ttgcactagg attatgtgtt gcccatagcc ataaattcgt gtgagatgga
     6781 catccagtct ttacggcttg tccccacccc atggatttct attgttaaag atattcagaa
     6841 tgtttcattc ctacactagt atttattgcc caaggggttt gtgagggtta tattggtgtc
     6901 atagcacaat gccaccactg aaccccccgt ccaaatttta ttctgggggc gtcacctgaa
     6961 accttgtttt cgagcacctc acatacacct tactgttcac aactcagcag ttattctatt
     7021 agctaaacga aggagaatga agaagcaggc gaagattcag gagagttcac tgcccgctcc
     7081 ttgatcttca gccactgccc ttgtgactaa aatggttcac taccctcgtg gaatcctgac
     7141 cccatgtaaa taaaaccgtg acagctcatg gggtgggaga tatcgctgtt ccttaggacc
     7201 cttttactaa ccctaattcg atagcatatg cttcccgttg ggtaacatat gctattgaat
     7261 tagggttagt ctggatagta tatactacta cccgggaagc atatgctacc cgtttagggt
     7321 taacaagggg gccttataaa cactattgct aatgccctct tgagggtccg cttatcggta
     7381 gctacacagg cccctctgat tgacgttggt gtagcctccc gtagtcttcc tgggcccctg
     7441 ggaggtacat gtcggccgca aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7501 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7561 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7621 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7681 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7741 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7801 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7921 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7981 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8041 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8101 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8161 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8221 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8281 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8341 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaactatgg
     8401 ttggcgcgtt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     8461 ctcatttttt aaccaataga ccgaaatcgg caaaatccct tataaatcaa aagaatagcc
     8521 cgagatagag ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     8581 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     8641 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     8701 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     8761 gaaagcgaaa ggagcgggcg ctaaggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     8821 caccacaccc gccgcgctta atgcgccgct acagggcgcg ccgatcttca atattggcca
     8881 ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     8941 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     9001 tgtt
//
