LOCUS       sequence-273881-        9773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag PLK1
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    805..1004
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1205..1621
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1551..1573
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1589..1605
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..1686
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1741..1765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3573..3597)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3656..4117
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3712..3729)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3939..3958
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4122..4916
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4176..4195)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4786..4805
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5103..5572
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5608..5712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5713..5781,5782..6573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5931..5950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8339..8927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8828..8847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9081..9098
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9202..9629)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9312..9333)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9524..9543
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgcg
      601 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
      661 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
      721 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
      781 accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg
      841 tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg
      901 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
      961 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     1021 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     1081 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     1141 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1201 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1261 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1321 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1381 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1441 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1501 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1561 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1621 cggccggatc cagaattcgc caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
     1681 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
     1741 acaagtttgt acaaaaaagt tggcatgagt gctgcagtga ctgcagggaa gctggcacgg
     1801 gcaccggccg accctgggaa agccggggtc cccggagttg cagctcccgg agctccggcg
     1861 gcggctccac cggcgaaaga gatcccggag gtcctagtgg acccacgcag ccggcggcgc
     1921 tatgtgcggg gccgcttttt gggcaagggc ggctttgcca agtgcttcga gatctcggac
     1981 gcggacacca aggaggtgtt cgcgggcaag attgtgccta agtctctgct gctcaagccg
     2041 caccagaggg agaagatgtc catggaaata tccattcacc gcagcctcgc ccaccagcac
     2101 gtcgtaggat tccacggctt tttcgaggac aacgacttcg tgttcgtggt gttggagctc
     2161 tgccgccgga ggtctctcct ggagctgcac aagaggagga aagccctgac tgagcctgag
     2221 gcccgatact acctacggca aattgtgctt ggctgccagt acctgcaccg aaaccgagtt
     2281 attcatcgag acctcaagct gggcaacctt ttcctgaatg aagatctgga ggtgaaaata
     2341 ggggattttg gactggcaac caaagtcgaa tatgacgggg agaggaagaa gaccctgtgt
     2401 gggactccta attacatagc tcccgaggtg ctgagcaaga aagggcacag tttcgaggtg
     2461 gatgtgtggt ccattgggtg tatcatgtat accttgttag tgggcaaacc accttttgag
     2521 acttcttgcc taaaagagac ctacctccgg atcaagaaga atgaatacag tattcccaag
     2581 cacatcaacc ccgtggccgc ctccctcatc cagaagatgc ttcagacaga tcccactgcc
     2641 cgcccaacca ttaacgagct gcttaatgac gagttcttta cttctggcta tatccctgcc
     2701 cgtctcccca tcacctgcct gaccattcca ccaaggtttt cgattgctcc cagcagcctg
     2761 gaccccagca accggaagcc cctcacagtc ctcaataaag gcttggagaa ccccctgcct
     2821 gagcgtcccc gggaaaaaga agaaccagtg gttcgagaga caggtgaggt ggtcgactgc
     2881 cacctcagtg acatgctgca gcagctgcac agtgtcaatg cctccaagcc ctcggagcgt
     2941 gggctggtca ggcaagagga ggctgaggat cctgcctgca tccccatctt ctgggtcagc
     3001 aagtgggtgg actattcgga caagtacggc cttgggtatc agctctgtga taacagcgtg
     3061 ggggtgctct tcaatgactc aacacgcctc atcctctaca atgatggtga cagcctgcag
     3121 tacatagagc gtgacggcac tgagtcctac ctcaccgtga gttcccatcc caactccttg
     3181 atgaagaaga tcaccctcct taaatatttc cgcaattaca tgagcgagca cttgctgaag
     3241 gcaggtgcca acatcacgcc gcgcgaaggt gatgagctcg cccggctgcc ctacctacgg
     3301 acctggttcc gcacccgcag cgccatcatc ctgcacctca gcaacggcag cgtgcagatc
     3361 aacttcttcc aggatcacac caagctcatc ttgtgcccac tgatggcagc cgtgacctac
     3421 atcgacgaga agcgggactt ccgcacatac cgcctgagtc tcctggagga gtacggctgc
     3481 tgcaaggagc tggccagccg gctccgctac gcccgcacta tggtggacaa gctgctgagc
     3541 tcacgctcgg ccagcaaccg ttcaggcctc ctacccaact ttcttgtaca aagtggtttg
     3601 atctagtcga ccaattccgg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     3661 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     3721 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     3781 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     3841 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     3901 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     3961 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4021 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca
     4081 cggggacgtg gttttccttt gaaaaacacg atgataatac catgattgaa caagatggat
     4141 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4201 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4261 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     4321 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4381 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4441 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4501 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4561 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4621 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     4681 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4741 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4801 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4861 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     4921 gactctgggg ttcgcatcga taaaataaaa gattttattt agtctccaga aaaagggggg
     4981 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     5041 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
     5101 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     5161 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     5221 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     5281 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     5341 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     5401 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     5461 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     5521 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt cacaggccag
     5581 tggggccgca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5941 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6001 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6061 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6121 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6181 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6241 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6301 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6361 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6421 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6481 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6541 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6601 ctttagattg atttgcggcc gcgaaaagga caagcagcga aaattcacgc ccccttggga
     6661 ggtggcggca tatgcaaagg atagcactcc cactctacta ctgggtatca tatgctgact
     6721 gtatatgcat gaggatagca tatgctaccc ggatacagat taggatagca tatactaccc
     6781 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
     6841 agatataaat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
     6901 agatatagat taggatagcc tatgctaccc agatatagat taggatagca tatgctatcc
     6961 agatatttgg gtagtatatg ctacccatgg caacattagc ccaccgtgct ctcagcgacc
     7021 tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg tgtgtaattt
     7081 gtcctccaga tcgcagcaat cgcgccccta tcttggcccg cccacctact tatgcaggta
     7141 ttccccgggg tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt ggttagcggg
     7201 gttacaatca gccaagttat tacaccctta ttttacagtc caaaaccgca gggcggcgtg
     7261 tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc acttgtcttt
     7321 gtttatgggc cccattggcg tggagccccg tttaattttc gggggtgtta gagacaacca
     7381 gtggagtccg ctgctgtcgg cgtccactct ctttcccctt gttacaaata gagtgtaaca
     7441 acatggttca cctgtcttgg tccctgcctg ggacacatct taataacccc agtatcatat
     7501 tgcactagga ttatgtgttg cccatagcca taaattcgtg tgagatggac atccagtctt
     7561 tacggcttgt ccccacccca tggatttcta ttgttaaaga tattcagaat gtttcattcc
     7621 tacactagta tttattgccc aaggggtttg tgagggttat attggtgtca tagcacaatg
     7681 ccaccactga accccccgtc caaattttat tctgggggcg tcacctgaaa ccttgttttc
     7741 gagcacctca catacacctt actgttcaca actcagcagt tattctatta gctaaacgaa
     7801 ggagaatgaa gaagcaggcg aagattcagg agagttcact gcccgctcct tgatcttcag
     7861 ccactgccct tgtgactaaa atggttcact accctcgtgg aatcctgacc ccatgtaaat
     7921 aaaaccgtga cagctcatgg ggtgggagat atcgctgttc cttaggaccc ttttactaac
     7981 cctaattcga tagcatatgc ttcccgttgg gtaacatatg ctattgaatt agggttagtc
     8041 tggatagtat atactactac ccgggaagca tatgctaccc gtttagggtt aacaaggggg
     8101 ccttataaac actattgcta atgccctctt gagggtccgc ttatcggtag ctacacaggc
     8161 ccctctgatt gacgttggtg tagcctcccg tagtcttcct gggcccctgg gaggtacatg
     8221 tcggccgcaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8281 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8461 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8701 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8821 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8881 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9001 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9061 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9121 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caactatggt tggcgcgttg
     9181 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     9241 accaatagac cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagagt
     9301 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     9361 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa
     9421 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     9481 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     9541 gagcgggcgc taaggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     9601 ccgcgcttaa tgcgccgcta cagggcgcgc cgatcttcaa tattggccat tagccatatt
     9661 attcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
     9721 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gtt
//
