LOCUS       sequence-27389-e         311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 311)
  TITLE     Garbers lab plasmids
REFERENCE   2  (bases 1 to 311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLLU2G-Dazl2
FEATURES             Location/Qualifiers
     source          1..311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    159..192
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(234..250)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..251)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gcctcgnacg ngggcggagg atcaggacgc tcgctgcgcc cttcgtctga cgtggcagcg
       61 ctcgccgntg aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccagat
      121 cccgggcccg cgggccgctt aagcttggaa cccttaatat aacttcgtat aatgtatgct
      181 atacgaagtt attaggtccc tcgacctgct ggaatctcgt gaagcgagct tatcgatacc
      241 gtcgacctcg agggtcaccg cgcgttaaaa aagttgatcc aggagctgac ctctcttgaa
      301 ngtnnnctca a
//
