LOCUS       sequence-273939-       12679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2,
            and N3 are removed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 12679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz76_CB_operon_CsoS2_N4
FEATURES             Location/Qualifiers
     source          1..12679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1228..1314
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1406..1433
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1455..1474
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(1489..1494,1495..1512,1513..1519)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1527..1543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        9393..9494
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             9495..10154
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9561..9580)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        10219..10296
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             10297..11379
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(10316..10335)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      complement(join(12364..12679,1..273))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12444..12463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
       61 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      301 tctgacgctc agtctagttc agccagctcg tcgtgatgtc gaaacccaag ccaccctcag
      361 aggtgaaggc cgcttcgagc acatcgggaa gcgtgtcgac gacggtgctc ggtgcctcgg
      421 gtaagagcca gatagatgcg gtgacgttca ccgtcgcgat cgtggcggga accacggtca
      481 ctgcgtccgt tacaactcgc acgtcgtcgg ccagcacgac tgactcgacg gcttcgatta
      541 gcccgggaga ggcaaggcca tcaggctcgg tagacagaat gctgattaac acctcgccgg
      601 gagcagggct gctcacagcc gcgtccttca cccgtgggtc agccgtcagc gcttgatagc
      661 ggtaccaggc cgcaccgcct gcggtcgagc tgcccttgat ccgctcgatg gtgcgatcgc
      721 gaagctcgcc atccggctca ttcggctgcc gcaccacgcc gtagaacgcg gcaaggttgt
      781 cgaggtcggg gccgtttgag tagcgaagca gtgtcgcaag aagtgcgtcg ttgatccgct
      841 gacgcaggat cagctcgcgg gctgcgcaga cctccagcag cttgatgacc gggtcggatt
      901 cgaggatggc ggtgtagctg gcgtcacgcg atcgcaggtc gtcgatcaag tcctgcagga
      961 tcagttcaaa gtccagcgct tcgatgatgg tgggcgcggg aatcgtagca aagtcaagaa
     1021 cggtcatgag acgactaagc cctccagcgt gatacgcctg ccctcgggga tgtagtagcc
     1081 gatcaggttc agctcgactt gaccagctgc gctggctgag acgatgcgaa ccttctccag
     1141 cttcagccgt ggctcccagc gatcgagcgc ttcagctgtg gccgccacca ggtcaacgat
     1201 gagggactgg ttgatcggtc tagtcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1261 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1321 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     1381 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     1441 ctactctagt ttcgaattgt gagcgctcac aatttcgaaa ccccaggctt gacactttat
     1501 gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcagc taggtgcgcg
     1561 aatcccccat cctttcagga ggaactcatg gcagttaaaa agtatagtgc tggtgtaaaa
     1621 gaataccggc agacctattg gatgccggaa tacacaccgt tggattccga catccttgca
     1681 tgcttcaaaa tcaccccaca accgggtgtt gatcgcgaag aagccgcagc cgcggttgca
     1741 gcagaatctt caaccggcac atggaccacc gtgtggaccg atttgctgac cgacatggac
     1801 tactacaaag gccgtgccta tcgcattgaa gacgtacccg gtgacgatgc ggcattctat
     1861 gcctttatcg cctacccaat cgacctgttc gaagaagggt cagttgttaa cgtgtttacc
     1921 tcactggttg gtaacgtgtt cggcttcaaa gcggtacgcg gcctgcgtct ggaagatgtt
     1981 cgcttcccac tcgcctacgt taaaacctgt ggcggcccac cgcacggtat tcaagtcgaa
     2041 cgcgacaaga tgaacaaata tggtcgccca ctgttgggtt gcaccatcaa gccaaaactt
     2101 ggtttgtctg cgaaaaacta cggccgtgcc gtatacgagt gcctccgtgg cggcctcgac
     2161 ttcactaaag atgatgaaaa catcaattct cagccgttca tgcgctggcg cgatcgcttc
     2221 ttgttcgtac aagacgcgac cgaaactgct gaagcccaaa ccggcgaacg caaaggccat
     2281 tacctcaacg taacggcgcc aactcctgaa gaaatgtaca agcgcgcaga attcgccaaa
     2341 gaaattggcg cgccaatcat tatgcacgac tacatcaccg gtggcttcac ggccaacact
     2401 ggcttggcca agtggtgtca agacaacggc gtactgctgc acatccaccg tgcgatgcat
     2461 gcggttatcg accgtaaccc gaaccacggt attcacttcc gtgttctgac caagattctg
     2521 cgtttgtcgg gtggcgatca cctgcacacc ggtaccgttg tcggcaaact ggaaggcgac
     2581 cgtgcctcta ctctgggctg gattgatttg ctccgcgaat cgtttatccc tgaagatcgc
     2641 tcgcgcggta tcttcttcga tcaagactgg ggttcaatgc caggcgtatt cgctgtggcc
     2701 tctggtggta ttcacgtatg gcacatgcct gcgctggtta acatctttgg tgacgactct
     2761 gtcctccaat tcggtggcgg tacgctgggt catccatggg gcaacgctgc cggtgctgct
     2821 gccaaccgtg ttgctctgga agcctgcgta gaagcgcgta accaaggccg cgatatcgaa
     2881 aaagaaggca aagaaattct gactgctgct gcacagcaca gcccagaact gaagattgcc
     2941 atggaaactt ggaaagagat caaattcgaa tttgacactg tcgacaaact cgacactcaa
     3001 aatcgttgat ccctcgtacc acacaacata ctaaggtgag taaccatggc tgaaatgcag
     3061 gattacaagc aaagcctcaa atatgagact ttctcttatc ttccacccat gaacgcggaa
     3121 cgcatccgcg ctcaaatcaa gtacgcaatt gctcaaggct ggagccccgg cattgagcac
     3181 gtagaagtga aaaactccat gaaccaatat tggtacatgt ggaaacttcc cttcttcggc
     3241 gaacaaaatg tcgacaacgt gttggctgaa attgaagcgt gtcgtagtgc gtatccaaca
     3301 caccaggtca aactggtggc ttatgacaac tatgcgcaaa gcttaggtct ggccttcgtg
     3361 gtctaccgcg gcaactaagt caagtctgtc attgcgctgt gcgtttctct acgcacagca
     3421 cttattcaag atggggtcaa acatgcgcgc cgtgccgcca aagccgcaat ctcaaggtgg
     3481 tccaggacgc aatggctatc aaattgatgg atacctagat accgcacttc atggccgcga
     3541 tgccgccaag cgccaccgtg aaatgctctg tcaatacggc cgcggcacag caccttcctg
     3601 caagccaaca ggccgtgtca aaaattctgt acagtcgggc aacgcagcgc caaaaaaggt
     3661 tgaaaccggt cacaccctat ccggcggatc tgttacgggc acgcaagtgg atcgtaaatc
     3721 tcatgtgact ggaaacgagc cgggcacttg ccgagcagtc acgggcaccg agtacgtagg
     3781 tactgagcaa ttcacctctt tttgcaatac cagccccaag ccaaatgcga cgaaggtcaa
     3841 tgtgaccaca acggctcgtg gtcgccctgt tagcggtacg gaagtttcac ggaccgagaa
     3901 ggtaactggc aacgaatccg gtgtctgccg taacgttacc ggcaccgaat acatgagtaa
     3961 tgaagctcac ttttctctat gtggaacagc cgcaaagcct tcacaagcgg ataaagtcat
     4021 gttcggcgcc acagcacgaa cgcatcaagt ggtcagtggc agtgatgaat tcaggccctc
     4081 ttctgttacg ggtaacgaat cgggtgcaaa acgcacaatt accggctcgc agtacgcaga
     4141 cgaaggtctt gcgcgactca cgatcaacgg agcacctgca aaagtagcca gaacccacac
     4201 ctttgcgggc tctgacgtta ccggcacgga aatcggtcgc tctactcgcg taactggtga
     4261 tgaaagcggt tcgtgtcgtt caatctcagg caccgagtat ctcagtaacg agcaattcca
     4321 atccttttgt gacacaaaac ctcaacgcag cccgttcaag gttggccaag atcgcacgaa
     4381 caagggtcag tctgtgactg gtaacttggt tgatcgttcc gaactggtta caggtaacga
     4441 accaggttca tgctctcggg ttacaggctc tcagtatggc caaagcaaaa tctgcggtgg
     4501 tggcgtggga aaagtgcgct caatgcgcac ccttcgcggc acctcagtat ctggccaaca
     4561 gctagatcat gccccaaaga tgtccggtga cgagcgcggc gggtgcatgc ccgtcaccgg
     4621 taatgagtac tacggtcgtg aacatttcga accgttttgt acgagcaccc cagagcccga
     4681 agctcaatca actgaacaat cattgacctg tgaaggacaa attattagcg gcacttcagt
     4741 tgacgccagt gatttggtca caggaaatga aatcggtgaa cagcaactca tcagcggtga
     4801 cgcctatgtt ggcgcgcagc agacaggttg ccttcccact agtccacgct tcaaccaaac
     4861 tggcaatgtt cagtcaatgg gttttaagaa caccaatcag ccagaacaaa actttgcacc
     4921 aggtgaagta atgcctactg actttagtat tcaaacccca gctcgctcgg ctcagaatcg
     4981 cattacaggt aacgacattg cgccctcagg tcgcattaca ggccctggta tgctggcaac
     5041 cggcttgatt acaggaaccc ccgaattcag gcacgctgcg cgcgagttgg ttggttctcc
     5101 acaacccatg gcaatggcca tggccaaccg taataaggct gctcaagcac ctgttgtgca
     5161 gccagaagtg gttgcaactc aggaaaagcc tgagttggta tgtgcaccaa gaagcgatca
     5221 aatggatcgt gtgagtggcg aaggcaaaga acgttgccac atcactggcg atgactggtc
     5281 agttaacaag cacatcaccg gtacagccgg tcaatgggcg agtggtcgca acccttccat
     5341 gcgcggtaat gcgcgtgtgg tcgaaaccag cgcgtttgcc aatcgcaatg tgccaaaacc
     5401 tgaaaagccg ggctccaaga tcacgggcag tagtggtaat gacacccaag gtagtctgat
     5461 cacttactcc ggcggcgcgc gcggttgagc gttaagtaaa gtgtaacgat catgaacacc
     5521 cgtaacacac gaagcaagca acgcgcaccg tttggtgtta gctcatcagt caaacctcgg
     5581 cttgacttga ttgagcaagc accaaaccct gcctatgacc gccatcctgc ttgcataacg
     5641 ttgcctgagc gtacctgccg gcacccgtta actgaccttg aagccaacga acaactgggt
     5701 cgttgcgagg atagcgtcaa gaaccgtttt gatcgcgtta tccctttctt gcaagttgtt
     5761 gctggcattc ctcttggtct ggattatgtt acccgtgttc aagaattagc ccagtcgtcg
     5821 cttggacata cgctgcccga agaattactc aaagataatt ggatcagtgg gcacaattta
     5881 aaaggcattt ttggctacgc gactgctaaa gcactaacag cagccacgga acaattcagt
     5941 cgtaaaataa tgtctgagaa ggacgattcc gcatcggcca ttggcttctt cctggattgc
     6001 gggttccacg cagtggacat aagcccttgt gccgatggtc gtctcaaagg gttactgcct
     6061 tacatattgc gtttgcccct tacggcattc acctatcgta aagcctacgc aggctcgatg
     6121 ttcgatattg aagatgatct ggcacagtgg gagaaaaatg aactccgccg ttatcgtgaa
     6181 ggtgttccaa atacagcgga tcagccaaca cgatacctga aaattgctgt gtatcatttc
     6241 agcacctctg acccgacaca ctctggctgc gcggcacacg gcagtaatga tcgtgcagca
     6301 ctggaagcgg ctttaaccca gctgatgaaa ttcagagaag cggttgaaaa tgcccattgc
     6361 tgcggcgcaa gtatcgatat tttactgatt ggcgttgata cggatacgga tgccattcgc
     6421 gttcatattc cggatagcaa aggttttttg aatccgtatc gttatgttga caacacagta
     6481 acttatgcgc aaacactaca tctggcgccg gatgaggctc gtgtgattat tcacgaagca
     6541 attctcaacg caaaccgcag cgatggttgg gctaaaggaa atggagtagc cagcgagggg
     6601 atgcgtcgtt ttattggtca gcttttgatc aacaacctct cgcaaatcga ttacgtagta
     6661 aatcgtcatg gtggtcgcta tccacccaat gatattggtc atgctgagcg atatatcagt
     6721 gttggtgatg gttttgatga agttcaaatc cggaatttag cctactacgc gcatttggat
     6781 acggttgaag aaaatgcgat tgatgtggat gtgggaatca aaattttcac caaacttaat
     6841 ttgagtcgag gtttaccgat tccgattgcc atccactatc gctatgaccc caatgttcca
     6901 ggctccagag aaagaaccgt ggtcaaagca agacggatat acaacgccat taaagagcgg
     6961 ttctcatcct tggatgagca gaatctattg cagtttcgtt tgagcgttca ggcgcaggat
     7021 atcggaagcc cgattgaaga ggttgcatcc gcatgaaaat catgcaagtt gagaaaacgt
     7081 tggtttcaac aaaccgtatt gctgatatgg gtcacaaacc actattagtg gtatgggaga
     7141 aaccgggcgc gcccaggcag gtcgccgtgg atgcgattgg ctgcataccg ggcgattggg
     7201 ttttgtgcgt tgggtcatcg gcagcacgag aggctgcagg aagcaagtct tatccctctg
     7261 atttgacgat tatcgggatt attgatcagt ggaatggtga gtaatggaag taatgcgcgt
     7321 tcgttccgac ctaatcgcaa cacgcaggat tcccggtctt aaaaatatct ctttgcgtgt
     7381 catggaggat gctacgggta aggtcagtgt cgcttgcgat cccattggcg ttcccgaggg
     7441 atgttgggtc tttacgatta gcggctctgc cgctcggttt ggcgtgggtg attttgagat
     7501 tctcacggat ttgacgattg gtggcatcat cgatcactgg gtaacttgac catcgctaga
     7561 tgagttgatt ttgaatgagt ctttattgag gagagaagaa atggcagcag taacaggtat
     7621 tgcactgggt atgattgaaa cacgtggtct ggttccagcg attgaagctg ccgatgccat
     7681 gaccaaggcc gccgaagtac gtttggttgg ccgtcaattt gttggtggtg gttacgtgac
     7741 cgttttggtc cgtggtgaaa ccggtgccgt caacgcagca gttcgtgcgg gcgctgatgc
     7801 ctgcgaacga gtcggcgatg gtctggtcgc ggcgcatatc attgcccgtg tccattccga
     7861 agtcgaaaac atcctgccga aagcccctga agcttaagga ttgggaaaga cgaaccggcg
     7921 caggcttgtt ccggttcttt gcataaagtg acagcttagg agtttattta aatggctgat
     7981 gtaactggta ttgctctggg tatgatcgaa acacgtggct tagttcctgc gattgaagca
     8041 gcggacgcca tgactaaagc ggctgaagtg cgtttggtcg gtcgtcaatt tgttggtggc
     8101 ggttacgtca ccgtattggt tcggggcgaa acaggcgctg ttaacgccgc tgttcgtgct
     8161 ggcgccgatg cttgcgaacg tgttggtgat ggcttggttg ctgcgcacat cattgcgcgt
     8221 gtccactcag aagtagaaaa cattctgcct aaggcgccac aagcctaagt cagatattcc
     8281 taagacggct cacttcggcg acacccgtcg ccgaaaccgt ctaaccaaat ttctgggctc
     8341 gttctgaacg tgctcaacac tagttttaga ggatctgtta tggcaacgac tcacggtatt
     8401 gccctgggca tgattgaaac acgaggattg gttcctgcca ttgaagccgc agatgccatg
     8461 accaaagcgg cggaagtccg tctggtcgga cgatcatttg ttggcggcgg ttacgtgacc
     8521 gtaatggttc gtggtgagac aggtgcagta aatgctgccg ttcgtgcggg tgctgacgcc
     8581 tgtgaacgtg ttggcgatgg cctggttgct gcgcacatca ttgcgcgcgt tcattctgaa
     8641 gttgagatca tcctacccga gacgcccgaa gactcagatt ccgcgtggtg tatcgcaaat
     8701 ctgaatagct aatgtctagt agggaagatg cgcatgaaca acattgattt gcgcgtctat
     8761 tcgtttatcg attcgcttca accgcagctt gcatcctatc ttgcgacatc atcgcaaggc
     8821 tttcttcccg ttccgggtga tgcctgcttg tggattgaag tcgcgccggg catggccgtc
     8881 catcgcctca gtgatattgc gctaaaggcc acgaacgttc gtctcggcga acaggtagtc
     8941 gagcgtgctt ttggctcgat ggaaattcat taccgaaacc aaagcgacgt tctcgcatcc
     9001 ggtgaggccg ttttaagaga aatcaatcac gcgcaagaag atcgtctgcc ttgtcgcatc
     9061 gcatggaaag agatcattcg agcgattacc cccgatcatg ccaccttgat caatcgccag
     9121 ttgcgtaaag gctctatgct gttgccgggc aaaagcatgt tcatccttga aacagaaccg
     9181 gcaggttata ttgttcaggc tgccaacgag gccgagaaag cagctcatgt gactctgatc
     9241 gatgtacgtg cttttggtaa ctttggtcgc ctgaccatga tgggcagcga agcggaaacc
     9301 gaagaagcca tgcgggcggc tgaggccaca atcgcaagca tcaacgcgcg tgcgcgtcgc
     9361 gctgaagggt tctaagcggc cgcgtaggat ccgatcggca cgtaagaggt tccaactttc
     9421 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     9481 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     9541 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     9601 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     9661 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg
     9721 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     9781 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     9841 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     9901 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     9961 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcact atgggcaaat
    10021 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct
    10081 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
    10141 agggcggggc gtaatttttt taaggcagtt attggtgccc ttgaattcct actagtcgaa
    10201 gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga
    10261 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
    10321 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
    10381 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
    10441 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
    10501 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
    10561 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
    10621 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
    10681 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
    10741 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
    10801 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
    10861 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
    10921 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
    10981 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
    11041 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
    11101 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
    11161 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
    11221 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
    11281 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11341 ctggcacgac aggtttcccg actggaaagc gggcagtgat cccacagccg ccagttccgc
    11401 tggcggcatt ttaactttct ttaatgaatc tagtgacaag ccggggcaga cgtgagccgt
    11461 agtcccgtcg ccagacgcgg gtgcccacgg gcgtcgtcag gatgtccgta attgactgcc
    11521 ggaggtggtc aatgcccttc agctccttgc cactgtcacg gctcatgcct cgggtcatta
    11581 gtcgcccgct ccggtatctt cactggcttc gatgattgcc gccccgcagc tgcagaggtc
    11641 accgatccga gcagtcggcc tctggttggt aaagaccgtg cgactgccgg tgatgatcgt
    11701 gttcaagcca tgcagggggc aggcgtgaag gtcatccttt cgggccacgg ggcggctgtt
    11761 gacgaaggtg tcgtcgctcc cggtgatgat gatcccgccg tgatcggtca cgtcgtttag
    11821 tcgagcgatg cctggcgtcg tagtcacggg tttaggtcaa tacgacttgc ggtcactgta
    11881 acgttgccct cggcggtcac gttaacgtcg ccttgggctt cgacttgcgc ctcctgcaca
    11941 aggatcacaa tccgtccttg ggctgcggtg aggtcgatct tgtactcatg cgcttcgcgg
    12001 tcgtactgga tgattgagtc atcctcgaac tgcgtctttt ggatcgtttc tttgtcctcg
    12061 atctgggggt agtcagtcga gaacgcgccg ggcatcgcga agccctgact gatctcgccg
    12121 gagggggcca tcacgacgac ggcctcaccg acctcgggcg cccaccagaa ccgatccttg
    12181 cccgctcgct gcgtgagcca cggaatccag tcagtgagga gcagcgcctc gccgctctcc
    12241 tcgtcttcct cgatcgcgac acggatcagc cccttgggat agtcagcctc ggctaccctg
    12301 cctacgcgga gcaagttgcc gtgacgccga ctgtctcgag tatctagtcg cgttgctggc
    12361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    12421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    12481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    12541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    12601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    12661 taactatcgt cttgagtcc
//
