LOCUS       sequence-27399-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Kang MG, Guo Y, Huganir RL
  TITLE     AMPA receptor and GEF-H1/Lfc complex regulates dendritic spine 
            development through RhoA signaling cascade.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3. 106(9):3549-54.
  PUBMED    19208802
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Mar 3. 106(9):3549-54."
COMMENT     Sequence Label: lentiviral shRNA GEF-H1
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        146..164
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(262..476)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(322..341)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(496..512)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(496..512)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(544..563)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(545..563)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcgcttttc tgagcgtccg ggcggaggat caggacgctc gctgcgccct tcgtctgacg
       61 tggcagcgct cgccgtgagg aggggggcgc ccgcgggagg cgccaaaacc cggcgcggag
      121 gccagatctg gagccgacac gggttaatta accctcacta aagggaacaa aagctggtac
      181 cgggcccccc ctcgagaaaa aaaaccttca atggctccat tgatctcttg aatcaatgga
      241 gccattgaag gttggggatc tgtggtctca tacagaactt ataagattcc caaatccaaa
      301 gacatttcac gtttatggtg atttcccaga acacatagcg acatgcaaat attgcagggc
      361 gccactcccc tgtccctcac agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc
      421 cgcctagtga cactgggccc gcgattcctt ggagcgggtt gatgacgtca gcgttcgaat
      481 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccaat
      541 tcgccctata gtgagtcgta ttattaatta accaaactgg atctctgctg tccctgtaat
      601 aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
      661 ttgctattat gtctactatt ctcccacgct gaaaaaaaaa taaatgcttt ttttctttta
      721 aaattgtgga tgaata
//
