LOCUS       sequence-274029-       10030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dimpLbCas12a-SunTag in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Toth E, Varga E, Kulcsar PI, Kocsis-Jutka V, Krausz SL, Nyeste A, 
            Welker Z, Huszar K, Ligeti Z, Talas A, Welker E
  TITLE     Improved LbCas12a variants with altered PAM specificities further 
            broaden the genome targeting range of Cas12a nucleases.
  JOURNAL   Nucleic Acids Res. 2020 Feb 28. pii: 5763098. doi: 
            10.1093/nar/gkaa110.
  PUBMED    32107556
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Feb 28. pii: 5763098. doi: 10.1093/nar/gkaa110."
COMMENT     Sequence Label: pTE2690
FEATURES             Location/Qualifiers
     source          1..10030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        114..493
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        494..697
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     648..668
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     742..761
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        742..760
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             789..809
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             813..833
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             837..857
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             879..4562
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMRGWDKDVETDRRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPRVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4650..4706
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4722..4778
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4794..4850
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4866..4922
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4938..4994
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5010..5066
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5082..5138
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5154..5210
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5226..5282
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5298..5354
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(5500..5517)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5506..5730
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5776..6204
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5863..5882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6073..6094
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6213..6233)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6218..6547
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6398..6533
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6460..6479
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6614..7408
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6668..6687)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7278..7297
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7582..7703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7619..7638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7673..7692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7752..7768)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7752..7768)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7765..7787)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7776..7792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7800..7806,7807..7824,7825..7830))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7845..7866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7983..8000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8154..8742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8234..8253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8913..9704,9705..9773))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9536..9555
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9774..9878)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9953..9972)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
       61 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      121 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      181 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      241 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      301 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      361 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      421 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      481 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      541 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      601 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      661 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      721 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggcgtcag
      781 ccaccatgcc caaaaagaag cggaaggtcg atccaaagaa aaaacgcaaa gtcgatccta
      841 agaaaaagag aaaggtggcg gccgccagta cggccaccat gagcaagctg gagaagttta
      901 caaactgcta ctccctgtct aagaccctga ggttcaaggc catccctgtg ggcaagaccc
      961 aggagaacat cgacaataag cggctgctgg tggaggacga gaagagagcc gaggattata
     1021 agggcgtgaa gaagctgctg gatcgctact atctgtcttt tatcaacgac gtgctgcaca
     1081 gcatcaagct gaagaatctg aacaattaca tcagcctgtt ccggaagaaa accagaaccg
     1141 agaaggagaa taaggagctg gagaacctgg agatcaatct gcggaaggag atcgccaagg
     1201 ccttcaaggg caacgagggc tacaagtccc tgtttaagaa ggatatcatc gagacaatcc
     1261 tgccagagtt cctggacgat aaggacgaga tcgccctggt gaacagcttc aatggcttta
     1321 ccacagcctt caccggcttc tttcgtaaca gagagaatat gttttccgag gaggccaaga
     1381 gcacatccat cgccttcagg tgtatcaacg agaatctgac ccgctacatc tctaatatgg
     1441 acatcttcga gaaggtggac gccatctttg ataagcacga ggtgcaggag atcaaggaga
     1501 agatcctgaa cagcgactat gatgtggagg atttctttga gggcgagttc tttaactttg
     1561 tgctgacaca ggagggcatc gacgtgtata acgccatcat cggcggcttc gtgaccgaga
     1621 gcggcgagaa gatcaagggc ctgaacgagt acatcaacct gtataatcag aaaaccaagc
     1681 agaagctgcc taagtttaag ccactgtata agcaggtgct gagcgatcgg gagtctctga
     1741 gcttctacgg cgagggctat acatccgatg aggaggtgct ggaggtgttt agaaacaccc
     1801 tgaacaagaa cagcgagatc ttcagctcca tcaagaagct ggagaagctg ttcaagaatt
     1861 ttgacgagta ctctagcgcc ggcatctttg tgaagaacgg ccccgccatc agcacaatct
     1921 ccaaggatat cttcggcgag tggaacgtga tccgggacaa gtggaatgcc gagtatgacg
     1981 atatccacct gaagaagaag gccgtggtga ccgagaagta cgaggacgat cggagaaagt
     2041 ccttcaagaa gatcggctcc ttttctctgg agcagctgca ggagtacgcc gacgccgatc
     2101 tgtctgtggt ggagaagctg aaggagatca tcatccagaa ggtggatgag atctacaagg
     2161 tgtatggctc ctctgagaag ctgttcgacg ccgattttgt gctggagaag agcctgaaga
     2221 agaacgacgc cgtggtggcc atcatgaagg acctgctgga ttctgtgaag agcttcgaga
     2281 attacatcaa ggccttcttt ggcgagggca aggagacaaa cagggacgag tccttctatg
     2341 gcgattttgt gctggcctac gacatcctgc tgaaggtgga ccacatctac gatgccatcc
     2401 gcaattatgt gacccagaag ccctactcta aggataagtt caagctgtat tttcagaacc
     2461 ctcagttcat gaggggctgg gacaaggatg tggagaccga tcggcgggcc accatcctga
     2521 gatacggctc caagtactat ctggccatca tggataagaa gtacgccaag tgcctgcaga
     2581 agatcgacaa ggacgatgtg aacggcaatt acgagaagat caactataag ctgctgcccg
     2641 gccctaataa gatgctgcca agggtgttct tttctaagaa gtggatggcc tactataacc
     2701 ccagcgagga catccagaag atctacaaga atggcacatt caagaagggc gatatgttta
     2761 acctgaatga ctgtcacaag ctgatcgact tctttaagga tagcatctcc cggtatccaa
     2821 agtggtccaa tgcctacgat ttcaactttt ctgagacaga gaagtataag gacatcgccg
     2881 gcttttacag agaggtggag gagcagggct ataaggtgag cttcgagtct gccagcaaga
     2941 aggaggtgga taagctggtg gaggagggca agctgtatat gttccagatc tataacaagg
     3001 acttttccga taagtctcac ggcacaccca atctgcacac catgtacttc aagctgctgt
     3061 ttgacgagaa caatcacgga cagatcaggc tgagcggagg agcagagctg ttcatgaggc
     3121 gcgcctccct gaagaaggag gagctggtgg tgcacccagc caactcccct atcgccaaca
     3181 agaatccaga taatcccaag aaaaccacaa ccctgtccta cgacgtgtat aaggataaga
     3241 ggttttctga ggaccagtac gagctgcaca tcccaatcgc catcaataag tgccccaaga
     3301 acatcttcaa gatcaataca gaggtgcgcg tgctgctgaa gcacgacgat aacccctatg
     3361 tgatcggcat cgcgaggggc gagcgcaatc tgctgtatat cgtggtggtg gacggcaagg
     3421 gcaacatcgt ggagcagtat tccctgaacg agatcatcaa caacttcaac ggcatcagga
     3481 tcaagacaga ttaccactct ctgctggaca agaaggagaa ggagaggttc gaggcccgcc
     3541 agaactggac ctccatcgag aatatcaagg agctgaaggc cggctatatc tctcaggtgg
     3601 tgcacaagat ctgcgagctg gtggagaagt acgatgccgt gatcgccctg gaggacctga
     3661 actctggctt taagaatagc cgcgtgaagg tggagaagca ggtgtatcag aagttcgaga
     3721 agatgctgat cgataagctg aactacatgg tggacaagaa gtctaatcct tgtgcaacag
     3781 gcggcgccct gaagggctat cagatcacca ataagttcga gagctttaag tccatgtcta
     3841 cccagaacgg cttcatcttt tacatccctg cctggctgac atccaagatc gatccatcta
     3901 ccggctttgt gaacctgctg aaaaccaagt ataccagcat cgccgattcc aagaagttca
     3961 tcagctcctt tgacaggatc atgtacgtgc ccgaggagga tctgttcgag tttgccctgg
     4021 actataagaa cttctctcgc acagacgccg attacatcaa gaagtggaag ctgtactcct
     4081 acggcaaccg gatcagaatc ttccggaatc ctaagaagaa caacgtgttc gactgggagg
     4141 aggtgtgcct gaccagcgcc tataaggagc tgttcaacaa gtacggcatc aattatcagc
     4201 agggcgatat cagagccctg ctgtgcgagc agtccgacaa ggccttctac tctagcttta
     4261 tggccctgat gagcctgatg ctgcagatgc ggaacagcat cacaggccgc accgacgtgg
     4321 attttctgat cagccctgtg aagaactccg acggcatctt ctacgatagc cggaactatg
     4381 aggcccagga gaatgccatc ctgccaaaga acgccgacgc caatggcgcc tataacatcg
     4441 ccagaaaggt gctgtgggcc atcggccagt tcaagaaggc cgaggacgag aagctggata
     4501 aggtgaagat cgccatctct aacaaggagt ggctggagta cgcccagacc agcgtgaagc
     4561 acgctagtgg gcacaagctt aatggcggtg gcggagggat ggatgctaag tcactaactg
     4621 gatccaacgg tccgactgac gccgcggaag aagaactttt gagcaagaat tatcatcttg
     4681 agaacgaagt ggctcgtctt aagaaaggtt ctggcagtgg agaagaactg ctttcaaaga
     4741 attaccacct ggaaaatgag gtagctagac tgaaaaaggg gagcggaagt ggggaggagt
     4801 tgctgagcaa aaattatcat ttggagaacg aagtagcacg actaaagaaa gggtccggat
     4861 cgggtgagga gttactctcg aaaaattatc atctcgaaaa cgaagtggct cggctaaaaa
     4921 agggcagtgg ttctggagaa gagctattat ctaaaaacta ccacctcgaa aatgaggtgg
     4981 cacgcttaaa aaagggaagt ggcagtggtg aagagctact atccaagaat tatcatcttg
     5041 agaacgaggt agcgcgtttg aagaagggtt ccggctcagg agaggaactg ctctcgaaga
     5101 actatcatct tgaaaatgag gtcgctcgat taaaaaaggg atcgggcagt ggtgaggaac
     5161 tactttcaaa gaattaccac ctcgaaaacg aagtagctcg attaaagaaa ggttcagggt
     5221 cgggtgaaga attactgagt aaaaattatc atctggaaaa tgaggtagcg agactaaaaa
     5281 aggggagtgg ttctggcgag gaattgctat cgaaaaatta tcatcttgag aacgaagttg
     5341 ctaggctcaa aaagggctca ggctcaggca ccgcggtaaa cataggtggt ggaaccggtc
     5401 cgatggatct acagcggccg tacaagtaag aattctgcag atatccagca cagtggcggc
     5461 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     5521 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5581 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5641 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     5701 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     5761 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5821 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5881 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5941 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6001 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6061 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6121 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6181 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     6241 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6301 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6361 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6421 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6481 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6541 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     6601 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     6661 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     6721 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     6781 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     6841 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     6901 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6961 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7021 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     7081 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     7141 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     7201 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     7261 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     7321 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     7381 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     7441 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7501 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     7561 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     7621 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7681 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7741 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7801 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7861 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7921 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7981 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8041 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8101 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8161 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8221 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8281 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8341 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8401 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8461 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8521 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8581 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8641 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8701 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8761 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8821 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8881 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8941 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9001 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9061 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9121 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9181 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9241 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9301 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9361 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9421 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9481 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9541 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9601 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9661 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9721 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9781 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9841 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9901 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     9961 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
    10021 agtagtgcgc
//
