LOCUS       sequence-274031-        3421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SapTrap mNG-GLO donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3421)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD400
FEATURES             Location/Qualifiers
     source          1..3421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(25..111)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     160..182
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     174..191
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     175..191
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    207..306
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1184..1283)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1300..1319)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1301..1319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1324..1340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1324..1340)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1442..1461)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1453..2262
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1520..1539)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2409..2997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2898..2917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3151..3168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3327..3354)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
       61 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
      121 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
      181 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
      241 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
      301 caggctgctc ttcgatggtc agcaaaggcg aggaagacaa catggcttct cttccagcaa
      361 ctcacgagct tcacattttc ggatccatca acggagtcga tttcgatatg gttggacagg
      421 gaactggaaa tccaaatgac ggttacgagg agctcaactt aaaatcgacg aaggtaagtt
      481 taaacatata tatactaact aaccctgatt atttaaattt tcagggagac cttcaattct
      541 caccatggat tttagtgccc cacattggat acggatttca tcaatatctt ccatatccag
      601 atggaatgag tccttttcaa gccgctatgg tcgatggatc cggataccaa gttcatcgaa
      661 caatgcaatt cgaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat
      721 tttcagacgg agcttccctt actgtaaact atcgttatac ctatgagggg tcccacatca
      781 agggcgaggc tcaagtcaag ggaactggat tcccagccga tggaccagtt atgaccaaca
      841 gtctcaccgc cgccgattgg tgccgctcca agaagaccta tccaaatgac aagaccatca
      901 tttcaacatt caaatggtct tacacaactg gaaacggaaa aaggtaagtt taaacatgat
      961 tttactaact aactaatctg atttaaattt tcagataccg atcgacagct cgtacaacgt
     1021 acacgttcgc caaaccaatg gctgccaact atttgaaaaa ccagccaatg tacgtgttcc
     1081 gtaagaccga gcttaagcac tctaaaactg aattaaactt caaggagtgg caaaaagctt
     1141 tcactgatgt tatgggaatg gacgagctgt acaagcgaag agcacccagc tttcttgtac
     1201 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     1261 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     1321 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     1381 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1441 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     1501 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1561 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1621 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1681 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1741 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1801 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1861 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1921 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     1981 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2041 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2101 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2161 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2221 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2281 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     2341 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2461 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2521 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2581 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2641 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2701 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2761 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2821 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2881 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2941 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3001 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3061 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3121 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3181 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3241 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     3301 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     3361 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     3421 c
//
