LOCUS       sequence-274032-       10473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD316
FEATURES             Location/Qualifiers
     source          1..10473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             283..588
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1536..1556
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    1574..1607
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             1617..1646
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3463..3786)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5704..5728
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5731..6756
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5731..6756
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6757..6781)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7134..7167
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7186..7212
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7213..7239
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7874..7890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7874..7890)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7887..7909)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7898..7914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7922..7928,7929..7946,7947..7952))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7967..7988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8105..8122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8276..8864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8356..8375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9035..9826,9827..9895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9658..9677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9896..10000)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10068..10086
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10124..10146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10246..10265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(10459..10473,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(10473,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcgc cggcactagt tacccgaagt atgtcaaaaa gaggtatgct
       61 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
      121 atgatgtcaa tatctccggt tcggtaagca caaccatgca gaatgaagcc cgtcgtctgc
      181 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
      241 aaatgaacgg ctcttttgcc gacgagaaca ggggctggtg aaatgcagtt taaggtttac
      301 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
      361 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
      421 ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
      481 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
      541 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
      601 cttatacaca gccagtctgc aggtcgacct agggagcatc gggagcctca ggagcatcga
      661 tggtctccaa aggagaagaa ctctttaccg gagtcgtccc aattctcgtc gagctcgacg
      721 gagacgtaaa cggacacaaa ttctcggttt ccggagaagg agaaggagat gctacttatg
      781 gaaaactcac ccttaaattc atttgcacca ccggaaagct cccagtgccg tggccgactt
      841 tggtgaccac cctctcctgg ggcgttcaat gcttcgcccg ctacccaggt aagtttggga
      901 atgtatattc aatgtaatga agttggagga ttttttcaga ccatatgaag caacatgact
      961 tcttcaaatc agccatgcca gaaggatacg tccaggagcg aacaattttc ttcaaggacg
     1021 atggaaacta caagactcgt gctgaagtca agtttgaagg agatactctc gtgaaccgca
     1081 ttgagctcaa ggtaagttta acatgggaat cgtgcttctg tttaaacaag aatggttttc
     1141 agggaatcga cttcaaagaa gatggaaaca ttcttggaca caagctcgaa tacaactact
     1201 tcagcgacaa cgtgtatatc accgccgaca agcaaaaaaa tggaatcaag gccaacttca
     1261 agattcgcca caacatcgaa gacgggggcg ttcaacttgc tgatcattat cagcaaaaca
     1321 ccccaattgg aggtaagttt gcattttgta ataatttgag aatccaaaaa cttgcttttt
     1381 cagatggacc agtcctcctc cctgataatc actacctttc cactcaatca aagttgagca
     1441 aggatccaaa tgagaaaaga gatcacatgg ttcttctcga gtttgtcacc gccgccggaa
     1501 tcacacttgg aatggacgag ctgtacaaga ccggtgagaa tctgtacttt caatccggaa
     1561 aggtaagttt aaaataactt cgtataatgt atactatacg aagttatttt cagggagagc
     1621 aaaagcttat ctccgaggag gacctctaag gatgatcgac gccaacgtcg ttgaattttc
     1681 aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt
     1741 tttttcttgc tttctaaaaa attaattcaa tccaaatcta aacatttttt tttctctttc
     1801 cgtctcccaa ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct
     1861 tctcgctttc atttcattag gacgtggggg gaattggtgg aagggggaaa cacacaaaag
     1921 gatgatggaa atgaaataag gacacacaat atgcaacaac attcaattca gaaatatgga
     1981 ggaaggttta aaagaaaaca taaaaatata tagaggagga aggaaactag ctgccctcga
     2041 ttgtctatct tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt
     2101 catttcccaa ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc
     2161 tacatccgta ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca
     2221 cactgtctcg tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat
     2281 ttcctaataa aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact
     2341 aggtgagtta catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag
     2401 atgtctgtaa aacttgcgtg ttatgtgacg gcttcggtca ctgtcgccac tcttatggta
     2461 tgcttcatga ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat
     2521 accgagatga atgtgttcag agtgagttaa attatatttt gaattttaaa taatttttaa
     2581 ttttctagca atctaccaat ggattgtgga aggacatagt tgtcatcgga agatctagca
     2641 agcgtgtccg ttgtcaatat gaagagacca acgctacccc aactccacat gctgatggat
     2701 ccccatctgc tccaccaggt caaccaccag cagttccacc agtcttcaac cagccaaaga
     2761 ctccaaatgg agccaatgga aatggaccaa cctgcaactg caatgctgat aacaagtgcc
     2821 cagctggacc atccggacca aagggagttc caggagttcc aggactcgac ggagttccag
     2881 gacttgacgg tgttccagga gttggagctg atgatatcgc tccacaacgc gagtctgtcg
     2941 gatgcttcac ttgcccacaa ggaccagttg gaccaccagg agctcttgga agaccaggac
     3001 cacgtggact tccaggacca agaggacaaa atggaaaccc aggaagagat ggacaaccag
     3061 gacatccagg agagcaagga tcatccggtc aaatcggaaa gatcggagag ccaggaccac
     3121 caggagagaa gggacgcgac gccgagcatc caatcggaag accaggacca aagggaccaa
     3181 gaggagatca aggaccaaca ggaccagctg gacagaacgg tcttcacgga ccaccaggag
     3241 agccaggaac cgttggacca gaaggaccat ctggaaagca aggacgtcaa ggaccagacg
     3301 gaacccaggg agagactgga ccagacggaa gaccaggaaa ggatgccgag tactgccagt
     3361 gcccagacaa gtctccacca tcagaggctg tcaacgccaa ccgtggatac agaaatatct
     3421 aaattgttgg tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc
     3481 acaataaagt ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg
     3541 ctcgcatttt tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt
     3601 gtgaaagtac atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact
     3661 tttcagtgac tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag
     3721 agaatttttt gacaaaaaga aagaagagtg atagagaaga agggaatgct tgaaaggatt
     3781 ttgcatttat cttaatcacc gtcttcgaga agacgaacca tggcgccagt ttcagaatcc
     3841 aagtttctaa tatagttcat cacaatgttg acgttggtcc aacctccggc ttgcataatc
     3901 tctgggatgg aaactccagc gcgagccatg tcacgggcag ctccgacacg ggcggaatgt
     3961 ccagaccaag ccaagtaacg ttgtccggaa tcatccttgg ctccgtagat gagacggtgg
     4021 gtagcttcga agattcctga aaatttattt tcagcttatt cttagctaat atgttgttta
     4081 aacttacctt caagagctcg agtcgagagt tgtgatgttg cactcggggc agcaactccg
     4141 ttcttgcgaa cacgacagaa caggtagttg tttggatcat cggcgactcc ggaaaccgaa
     4201 atccagcgct caacgagctt ggtaactcca agggagagcg ctttttcgac tccggcagtc
     4261 gagaccagcg tttttgtacg tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta
     4321 atatccttga cgcgaatgcg ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt
     4381 ccgaggaagg cgaggtttcg gatgtcctgg caacgatcgg aattttccat aagagaacgg
     4441 acctgatcga agtcggttct gaaaatttcg tcaagtctta ggattgaata cattaatatt
     4501 taaacttacc tttcgaaggc gagggcttgc ttggctctct ctccagcatc aacattttcc
     4561 ttgcggatac gtctcattac gagagatacg gcattagaat ctgatgggcg tggaagtcca
     4621 gatcggcgat ggagcatatt gagctgtccg agatgttgtt ggatcgtctt caccgccaat
     4681 ccacgagctt ggaggtagag caagtagtca cgaacgtctt ctggctcggc tgggaaccac
     4741 ttgcggttgt taagcttgca ccaggcagcc caagaacggc acactgagag aagcatcttc
     4801 caggtgtgct cggagaaggc ttgtctgtca cggaacatat ccatgaggtt cttgcgaact
     4861 tcatcacttg tcgcgtcaac tggaagtgct ggaagatttt gatgaacagt gagaagattt
     4921 gacatatttt cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc
     4981 cgctttcctt atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg
     5041 cattcgtttg aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg
     5101 tctctttatt gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa
     5161 caagcagctc gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa
     5221 taccgaacaa catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac
     5281 tttaccacta tttccgtcca gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc
     5341 gtaaatctac acacgcgtct cttccgtgcg agagtccaag ccagcagcca aattcgttga
     5401 ctgagtattc aacgtttata cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat
     5461 gttctttttt cgattttttc caaggttttg acaaatttta ccacgaattt tgctatgttt
     5521 tcaattaaaa aatatgttat tcaactgttt ctatgaggaa aataaggctt tgcatgtaat
     5581 tttcttattc agcataattt ttaattaatt tgaattttct gtcctaacgt ttattttgtt
     5641 ttcttggtta tgactgatct gaaattaatt tttgaatttt aaggtaatat gtcaggcggt
     5701 gccgcaagtt tgtacaaaaa agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct
     5761 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     5821 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     5881 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     5941 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     6001 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     6061 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     6121 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     6181 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     6241 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     6301 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     6361 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     6421 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     6481 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     6541 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     6601 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     6661 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     6721 cgacgcccca gcactcgtcc gagggcaaag gaatagaccc agctttcttg tacaaagtgg
     6781 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     6841 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     6901 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     6961 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     7021 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     7081 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttaataactt
     7141 cgtataatgt atactatacg aagttatttt cagggagccg gctcttatcc atacgatgtc
     7201 ccagattacg cttacccata tgacgttcca gactatgccg gaggactagt tcgacctgca
     7261 gactggctgt gtataaggga gcctgacatt tagattcccc agaacataag gttgatagcg
     7321 ttcttgatat cgttctcacg gtgagaaagg tcggcaacct cctctccgat gacggaaact
     7381 ggaacggaag ccatgtcggt ggtcatcata cgccaagact cgtctccgat gtgaacaact
     7441 gggtagagct cacgtggaac cttgtcggaa agaagacggg cggaagcaag tgggataacc
     7501 atacggcgtc ctggggtatc gatgatgtcg gattggacgt cgacgaaaag acggtagcga
     7561 ctctctcttt tataagtgta aaccttgaat tgcatttcac cagcccctgt tctcgtcggc
     7621 aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc
     7681 tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccgaacc
     7741 ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact
     7801 gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggttcct aggaaatcga
     7861 taccggtaac atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7921 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7981 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8101 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8701 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8941 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9001 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9061 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9121 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9181 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9241 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9301 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9361 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9421 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9481 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9541 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9601 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9661 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9721 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9781 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9841 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9901 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9961 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10021 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    10081 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    10141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    10201 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    10261 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    10321 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    10381 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    10441 gggtaacgcc agggttttcc cagtcacgac gtt
//
