LOCUS       sequence-274033-        8610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP FLP/FRT reporter piggyBac transposon (3x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-F-3xSV40pA-F-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        123..502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        504..781
                     /label=chicken beta-actin promoter
     intron          782..1798
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1722..1744
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1806..1825
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1858..1891)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2022..2143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2255..2376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2488..2609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2632..2665)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             join(2674..2676,2677..3387)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2674..3387
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3418..3420,3421..4134)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3418..4131
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4192..4212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4610..4621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4766..4783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4772..4996
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5350..5384
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5471..5490)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5472..5490)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5497..5514)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5497..5513)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5506..5528)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5655..6110)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5792..5813)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6004..6023
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6137..6241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6242..6310,6311..7102)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6460..6479)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7273..7861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7762..7781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8015..8032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8149..8170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8185..8190,8191..8208,8209..8215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8223..8239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8228..8250
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8247..8263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8247..8263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8282..8302
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8284..8302
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8366..8428
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tctcttttac aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa
       61 taagtaatta gcttaaccta tacataatag aaacaaatta tacttattaa ccggtcatgg
      121 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      361 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      421 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      481 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      541 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      601 gatgggggcg gggggggggg gggggcgcgc gccggggggg gggggggggg gggggggggg
      661 ggggggggsg rggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      721 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      781 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      841 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      901 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      961 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     1021 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     1081 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1141 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1201 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1261 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1321 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1381 ggggccgcct cgggccgggg rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg
     1441 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1501 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1561 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1621 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1681 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1741 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1801 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgctagcgaa
     1861 gttcctatac tttctagaga ataggaactt cccaccgcgg tggcggccgc tctagaatca
     1921 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     1981 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2041 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2101 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaactagaa tcataatcag
     2161 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2221 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2281 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2341 tagttgtggt ttgtccaaac tcatcaatgt atcttaacta gaatcataat cagccatacc
     2401 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2461 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     2521 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2581 ggtttgtcca aactcatcaa tgtatcttaa ctagtggatc tcatgcctag ggaagttcct
     2641 atactttcta gagaatagga acttcggcgc gccatgagca aaggagaaga acttttcact
     2701 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     2761 cgtggagagg gtgaaggtga tgctacaaac ggaaaactca cccttaaatt tatttgcact
     2821 actggaaaac tacctgttcc gtggccaaca cttgtcacta ctctgaccta tggtgttcaa
     2881 tgcttttccc gttatccgga tcacatgaaa cggcatgact ttttcaagag tgccatgccc
     2941 gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaccta caagacgcgt
     3001 gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa gggtattgat
     3061 tttaaagaag atggaaacat tcttggacac aaactcgaat acaactttaa ctcacacaat
     3121 gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac
     3181 aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac tccaattggc
     3241 gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgt cctttcgaaa
     3301 gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc tgctgggatt
     3361 acacatggca tggatgagct ctacaaaggt tctgctggat ctgcagcagg atctggtatg
     3421 agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     3481 gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgctac aaacggaaaa
     3541 ctcaccctta aatttatttg cactactgga aaactacctg ttccgtggcc aacacttgtc
     3601 actactctga cctatggtgt tcaatgcttt tcccgttatc cggatcacat gaaacggcat
     3661 gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat atctttcaaa
     3721 gatgacggga cctacaagac gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     3781 cgtatcgagt taaagggtat tgattttaaa gaagatggaa acattcttgg acacaaactc
     3841 gaatacaact ttaactcaca caatgtatac atcacggcag acaaacaaaa gaatggaatc
     3901 aaagctaact tcaaaattcg ccacaacgtt gaagatggtt ccgttcaact agcagaccat
     3961 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     4021 tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt
     4081 gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa atgattaatt
     4141 aaaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4201 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4261 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4321 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4381 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4441 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4501 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4561 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4621 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4681 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcagg atccactaga
     4741 gcggccgcct agagctcgct atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     4801 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     4861 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4921 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     4981 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgac tagagcttgc
     5041 ggaaccctta agctttttta ttatatatta gtcacgatat ctataacaag aaaatatata
     5101 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
     5161 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
     5221 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
     5281 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
     5341 tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa aagatttgcg
     5401 ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa aaccacattg
     5461 tgttgggtcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     5521 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5581 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5641 gaatggcgaa tggcaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     5701 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     5761 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     5821 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5881 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5941 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     6001 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     6061 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     6121 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6181 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6241 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6301 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6361 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6421 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6481 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6541 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6601 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6661 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6721 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6781 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6841 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6901 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6961 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7021 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7081 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7141 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7201 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7261 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7321 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7381 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7441 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7501 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7561 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7621 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7681 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7741 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7801 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7861 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7921 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7981 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8041 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8101 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8161 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8221 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8281 cgcaattaac cctcactaaa gggaacctcc cccggtattc acgacagcag gctgaataat
     8341 aaaaaaatta gaaactatta tttaacccta gaaagataat catattgtga cgtacgttaa
     8401 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
     8461 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
     8521 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
     8581 tctataaagt aacaaaactt ttaaacattc
//
