LOCUS       sequence-274037-       10468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD291
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1178..1501)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    3419..3443
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3446..4471
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3446..4471
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4472..4496)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4849..4882)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             4901..4990
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(5035..5340)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5613..5629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5613..5629)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5626..5648)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5637..5653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5661..5667,5668..5685,5686..5691))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5706..5727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5844..5861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6015..6603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6095..6114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6774..7565,7566..7634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7397..7416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7635..7739)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7807..7825
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7863..7885)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7985..8004
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8198..8220
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8212..8229
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8213..8229
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8495..8800
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             9721..9741
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(9759..9792)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             9802..9828
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aataaaagga gacgaatccg gtaggaaatc gacaagtctc tgcttctcga agactaggtg
       61 agttacattt ctttggccga attattttga aatcctaaat ttgataattt cagagatgtc
      121 tgtaaaactt gcgtgttatg tgacggcttc ggtcactgtc gccactctta tggtatgctt
      181 catgaccatg tcaaccatct actcggaggt tgatggattc agagagaagc ttgataccga
      241 gatgaatgtg ttcagagtga gttaaattat attttgaatt ttaaataatt tttaattttc
      301 tagcaatcta ccaatggatt gtggaaggac atagttgtca tcggaagatc tagcaagcgt
      361 gtccgttgtc aatatgaaga gaccaacgct accccaactc cacatgctga tggatcccca
      421 tctgctccac caggtcaacc accagcagtt ccaccagtct tcaaccagcc aaagactcca
      481 aatggagcca atggaaatgg accaacctgc aactgcaatg ctgataacaa gtgcccagct
      541 ggaccatccg gaccaaaggg agttccagga gttccaggac tcgacggagt tccaggactt
      601 gacggtgttc caggagttgg agctgatgat atcgctccac aacgcgagtc tgtcggatgc
      661 ttcacttgcc cacaaggacc agttggacca ccaggagctc ttggaagacc aggaccacgt
      721 ggacttccag gaccaagagg acaaaatgga aacccaggaa gagatggaca accaggacat
      781 ccaggagagc aaggatcatc cggtcaaatc ggaaagatcg gagagccagg accaccagga
      841 gagaagggac gcgacgccga gcatccaatc ggaagaccag gaccaaaggg accaagagga
      901 gatcaaggac caacaggacc agctggacag aacggtcttc acggaccacc aggagagcca
      961 ggaaccgttg gaccagaagg accatctgga aagcaaggac gtcaaggacc agacggaacc
     1021 cagggagaga ctggaccaga cggaagacca ggaaaggatg ccgagtactg ccagtgccca
     1081 gacaagtctc caccatcaga ggctgtcaac gccaaccgtg gatacagaaa tatctaaatt
     1141 gttggtgttt tctaataaaa tatttgagat tcagtgagac ttttttcttg gcggcacaat
     1201 aaagtttcct ctttttgttg ggtcactcaa cgaacacaat acaaatattt gagcgctcgc
     1261 atttttctca acctttcggg tttaaagcac ttcaaaatgg aattgaagag agattgtgaa
     1321 agtacatcag acagaatcac attttgatag cattcacttc actcagatgc aaacttttca
     1381 gtgactataa aaagtcataa aataataaca ttaaaaaagc aaataaatta gcgagagaat
     1441 tttttgacaa aaagaaagaa gagtgataga gaagaaggga atgcttgaaa ggattttgca
     1501 tttatcttaa tcaccgtctt cgagaagacg aaccatggcg ccagtttcag aatccaagtt
     1561 tctaatatag ttcatcacaa tgttgacgtt ggtccaacct ccggcttgca taatctctgg
     1621 gatggaaact ccagcgcgag ccatgtcacg ggcagctccg acacgggcgg aatgtccaga
     1681 ccaagccaag taacgttgtc cggaatcatc cttggctccg tagatgagac ggtgggtagc
     1741 ttcgaagatt cctgaaaatt tattttcagc ttattcttag ctaatatgtt gtttaaactt
     1801 accttcaaga gctcgagtcg agagttgtga tgttgcactc ggggcagcaa ctccgttctt
     1861 gcgaacacga cagaacaggt agttgtttgg atcatcggcg actccggaaa ccgaaatcca
     1921 gcgctcaacg agcttggtaa ctccaaggga gagcgctttt tcgactccgg cagtcgagac
     1981 cagcgttttt gtacgtccga tgtggatgag cattcttcct ccgtctgtgc gactaatatc
     2041 cttgacgcga atgcgggcaa tttcggcgat acggagaagt gtgttgtaag caattccgag
     2101 gaaggcgagg tttcggatgt cctggcaacg atcggaattt tccataagag aacggacctg
     2161 atcgaagtcg gttctgaaaa tttcgtcaag tcttaggatt gaatacatta atatttaaac
     2221 ttacctttcg aaggcgaggg cttgcttggc tctctctcca gcatcaacat tttccttgcg
     2281 gatacgtctc attacgagag atacggcatt agaatctgat gggcgtggaa gtccagatcg
     2341 gcgatggagc atattgagct gtccgagatg ttgttggatc gtcttcaccg ccaatccacg
     2401 agcttggagg tagagcaagt agtcacgaac gtcttctggc tcggctggga accacttgcg
     2461 gttgttaagc ttgcaccagg cagcccaaga acggcacact gagagaagca tcttccaggt
     2521 gtgctcggag aaggcttgtc tgtcacggaa catatccatg aggttcttgc gaacttcatc
     2581 acttgtcgcg tcaactggaa gtgctggaag attttgatga acagtgagaa gatttgacat
     2641 attttcgaag ttttttagat gcactagaac aaagcgtgtt ggcttcctct gagcccgctt
     2701 tccttatata cccgcattct gcagccttac agaatgttct agaaggtcct agatgcattc
     2761 gtttgaaaat actcccggtg ggtgcaaaga gacgcagacg gaaaatgtat ctgggtctct
     2821 ttattgtgta cactactttt ccatgtaccg aatgtgagtc gccctccttt tgcaacaagc
     2881 agctcgaatg ttctagaaaa aggtggaaaa tagtataaat accgttgaaa ataaataccg
     2941 aacaacattt gctctaattg tgaaattaga aatcttcaaa ctataatcat gtcactttac
     3001 cactatttcc gtccagctca acgttccgtt tttggtgatc atttttgctt tcgtcgtaaa
     3061 tctacacacg cgtctcttcc gtgcgagagt ccaagccagc agccaaattc gttgactgag
     3121 tattcaacgt ttatacgttg tcggcaacga gaaataggaa aatgcatcgg gaaatgttct
     3181 tttttcgatt ttttccaagg ttttgacaaa ttttaccacg aattttgcta tgttttcaat
     3241 taaaaaatat gttattcaac tgtttctatg aggaaaataa ggctttgcat gtaattttct
     3301 tattcagcat aatttttaat taatttgaat tttctgtcct aacgtttatt ttgttttctt
     3361 ggttatgact gatctgaaat taatttttga attttaaggt aatatgtcag gcggtgccgc
     3421 aagtttgtac aaaaaagcag gctccatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     3481 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     3541 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     3601 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     3661 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     3721 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     3781 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     3841 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     3901 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     3961 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     4021 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     4081 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     4141 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     4201 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     4261 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     4321 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     4381 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     4441 ccccagcact cgtccgaggg caaaggaata gacccagctt tcttgtacaa agtgggtcca
     4501 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     4561 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     4621 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     4681 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     4741 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     4801 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttaat aacttcgtat
     4861 aggatacttt atacgaagtt attttcaggg agccggatct gaacaaaaac ttatttctga
     4921 agaggatctt gagcaaaagc tcatctccga ggaggacctc gagcagaagt tgatcagcga
     4981 ggaagacttg ccggctcgac ctgcagactg gctgtgtata agggagcctg acatttatat
     5041 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
     5101 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca
     5161 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gggactttat ctgacagcag
     5221 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
     5281 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
     5341 ttcaccagcc cctgttctcg tcggcaaaag agccgttcat ttcaataaac cgggcgacct
     5401 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
     5461 tctgcatggt tgtgcttacc gaaccggaga tattgacatc atatatgcct tgagcaactg
     5521 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac
     5581 atacttcggg ttcctaggaa atcgataaca tggtcatagc tgtttcctgt gtgaaattgt
     5641 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5701 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5761 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5821 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7741 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7801 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     7861 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7921 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     7981 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     8041 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     8101 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8161 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8221 acggccagtc gccggcacta gttacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     8281 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     8341 aatatctccg gttcggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     8401 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     8461 ggctcttttg ccgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     8521 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     8581 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
     8641 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     8701 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     8761 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     8821 cagccagtct gcaggtcgac ctagggagca tcgggagcct caggagcatc gatggtttcc
     8881 gagttgatca aggagaatat gcacatgaaa ctctacatgg aaggaaccgt caacaatcat
     8941 catttcaaat gtacttcaga aggagaagga aaaccatacg agggaactca gaccatgaga
     9001 atcaaggctg ttgagggagg taagtttaaa aaatacgtgt tcataacttg atacgaatat
     9061 aaattttcag gaccacttcc attcgcgttc gacattcttg ccacttcgtt catgtatgga
     9121 agcaagactt tcattaatca cacacaaggg atcccggatt tcttcaagca atctttccca
     9181 gaaggattca catgggagag agttaccact tacgaggatg gaggagttct taccgccacc
     9241 caagacacat ccctccaagg taagtttaaa agattatagc aatgttcgtc taaatataat
     9301 aaattttcag atggatgtct gatctacaac gtcaagatca gaggagtcaa tttcccatca
     9361 aatggaccag ttatgcagaa gaagactttg ggctgggagg cttccaccga gaccctttac
     9421 ccagcagatg gaggtctcga gggccgcgct gatatggctc tcaaactcgt cggaggagga
     9481 cacttgatct gcaatttaaa aaccacctac cggtcgaagg taagtttaaa atcaattttt
     9541 aatagcttat ataataatac aaattttcag aagccagcca agaatctcaa aatgccagga
     9601 gtttactatg ttgaccgtcg tcttgagcgc attaaagagg ccgataagga aacgtacgtt
     9661 gaacaacatg aagttgccgt tgctcgatat tgtgatctcc catccaagct cggacatcgt
     9721 gagaatctgt actttcaatc cggaaaggta agtttaaaat aacttcgtat aggatacttt
     9781 atacgaagtt attttcaggg atacccatac gacgtcccag actacgccta aggatgatcg
     9841 acgccaacgt cgttgaattt tcaaatttta aatactgaat atttgttttt tttcctatta
     9901 tttatttatt ctctttgtgt tttttttctt gctttctaaa aaattaattc aatccaaatc
     9961 taaacatttt tttttctctt tccgtctccc aattcgtatt ccgctcctct catctgaaca
    10021 caatgtgcaa gtttatttat cttctcgctt tcatttcatt aggacgtggg gggaattggt
    10081 ggaaggggga aacacacaaa aggatgatgg aaatgaaata aggacacaca atatgcaaca
    10141 acattcaatt cagaaatatg gaggaaggtt taaaagaaaa cataaaaata tatagaggag
    10201 gaaggaaaac tagctgccct cgattgtcta tcttccattc tcaccaagtt tcaacaactt
    10261 tattcagtca ttcttctctg gttcatttcc caaccaatct tcccgaataa agcccatctg
    10321 tcctttcggc cacattcctc acctacatcc gtactcctta tctcccgttc ttatccagtc
    10381 ttttatcgtc tctgcgtctc gcacactgtc tcgtcggtaa tgtaaaaaaa aaacaaaaaa
    10441 aacttgaaaa cttgcggaaa gatttcct
//
