LOCUS       sequence-274039-       10472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10472)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD288
FEATURES             Location/Qualifiers
     source          1..10472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             387..692
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(740..763)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(767..790)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(794..817)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    836..869
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1222..1246
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1247..2272)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1247..2272)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(2275..2299)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4217..4540
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(6357..6386)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    6396..6429
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6447..6467)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(7400..7705)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7971..7988)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7971..7987)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7980..8002)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8196..8215)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8315..8337
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8375..8393)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8461..8565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8566..8634,8635..9426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8784..8803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9597..10185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10086..10105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10339..10356
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatgt
      121 tatcgattcc taggaacccg aagtatgtca aaaagaggta tgctatgaag cagcatatta
      181 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
      241 cggttcggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
      301 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
      361 tgccgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
      421 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
      481 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtcccccgt gaactttacc
      541 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      601 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      661 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      721 ctgcaggtcg aactagtctc ttgtcatcgt catccttgta atcacgcttg tcgtcgtcgt
      781 ccttgtagtc acgcttatcg tcatcgtctt tataatcaga tccggctccc tgaaaataac
      841 ttcgtataat gtatgctata cgaagttatt aaaaattgcg gtcataaact gaaacgtaac
      901 atatgataag gtattttgtg tgcggttttt tctatgatgt ttcaaaaaaa tttatcagaa
      961 gtaaaaaaca taagaagtgt acacagaaca ttgtgtagat tttgggatag aattattatt
     1021 aatgggggga gggagcacaa tttttttcga ctttttatta cgaattaatt ctatttttga
     1081 atctacacaa tttcattgtt agaggtgact taaaagaagc taaaaaacaa agaaattaag
     1141 agaagttttt tgataataac aaaaataggg ggtgggagca cagggagaaa gagcatgtag
     1201 ggatgttgaa gagtaattgg acccactttg tacaagaaag ctgggtctat tcctttgccc
     1261 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     1321 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     1381 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     1441 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
     1501 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     1561 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     1621 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     1681 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     1741 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     1801 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     1861 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
     1921 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     1981 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     2041 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     2101 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     2161 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     2221 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atggagcctg
     2281 cttttttgta caaacttgcg gcaccgcctg acatattacc ttaaaattca aaaattaatt
     2341 tcagatcagt cataaccaag aaaacaaaat aaacgttagg acagaaaatt caaattaatt
     2401 aaaaattatg ctgaataaga aaattacatg caaagcctta ttttcctcat agaaacagtt
     2461 gaataacata ttttttaatt gaaaacatag caaaattcgt ggtaaaattt gtcaaaacct
     2521 tggaaaaaat cgaaaaaaga acatttcccg atgcattttc ctatttctcg ttgccgacaa
     2581 cgtataaacg ttgaatactc agtcaacgaa tttggctgct ggcttggact ctcgcacgga
     2641 agagacgcgt gtgtagattt acgacgaaag caaaaatgat caccaaaaac ggaacgttga
     2701 gctggacgga aatagtggta aagtgacatg attatagttt gaagatttct aatttcacaa
     2761 ttagagcaaa tgttgttcgg tatttatttt caacggtatt tatactattt tccacctttt
     2821 tctagaacat tcgagctgct tgttgcaaaa ggagggcgac tcacattcgg tacatggaaa
     2881 agtagtgtac acaataaaga gacccagata cattttccgt ctgcgtctct ttgcacccac
     2941 cgggagtatt ttcaaacgaa tgcatctagg accttctaga acattctgta aggctgcaga
     3001 atgcgggtat ataaggaaag cgggctcaga ggaagccaac acgctttgtt ctagtgcatc
     3061 taaaaaactt cgaaaatatg tcaaatcttc tcactgttca tcaaaatctt ccagcacttc
     3121 cagttgacgc gacaagtgat gaagttcgca agaacctcat ggatatgttc cgtgacagac
     3181 aagccttctc cgagcacacc tggaagatgc ttctctcagt gtgccgttct tgggctgcct
     3241 ggtgcaagct taacaaccgc aagtggttcc cagccgagcc agaagacgtt cgtgactact
     3301 tgctctacct ccaagctcgt ggattggcgg tgaagacgat ccaacaacat ctcggacagc
     3361 tcaatatgct ccatcgccga tctggacttc cacgcccatc agattctaat gccgtatctc
     3421 tcgtaatgag acgtatccgc aaggaaaatg ttgatgctgg agagagagcc aagcaagccc
     3481 tcgccttcga aaggtaagtt taaatattaa tgtattcaat cctaagactt gacgaaattt
     3541 tcagaaccga cttcgatcag gtccgttctc ttatggaaaa ttccgatcgt tgccaggaca
     3601 tccgaaacct cgccttcctc ggaattgctt acaacacact tctccgtatc gccgaaattg
     3661 cccgcattcg cgtcaaggat attagtcgca cagacggagg aagaatgctc atccacatcg
     3721 gacgtacaaa aacgctggtc tcgactgccg gagtcgaaaa agcgctctcc cttggagtta
     3781 ccaagctcgt tgagcgctgg atttcggttt ccggagtcgc cgatgatcca aacaactacc
     3841 tgttctgtcg tgttcgcaag aacggagttg ctgccccgag tgcaacatca caactctcga
     3901 ctcgagctct tgaaggtaag tttaaacaac atattagcta agaataagct gaaaataaat
     3961 tttcaggaat cttcgaagct acccaccgtc tcatctacgg agccaaggat gattccggac
     4021 aacgttactt ggcttggtct ggacattccg cccgtgtcgg agctgcccgt gacatggctc
     4081 gcgctggagt ttccatccca gagattatgc aagccggagg ttggaccaac gtcaacattg
     4141 tgatgaacta tattagaaac ttggattctg aaactggcgc catggttcgt cttctcgaag
     4201 acggtgatta agataaatgc aaaatccttt caagcattcc cttcttctct atcactcttc
     4261 tttctttttg tcaaaaaatt ctctcgctaa tttatttgct tttttaatgt tattatttta
     4321 tgacttttta tagtcactga aaagtttgca tctgagtgaa gtgaatgcta tcaaaatgtg
     4381 attctgtctg atgtactttc acaatctctc ttcaattcca ttttgaagtg ctttaaaccc
     4441 gaaaggttga gaaaaatgcg agcgctcaaa tatttgtatt gtgttcgttg agtgacccaa
     4501 caaaaagagg aaactttatt gtgccgccaa gaaaaaagtc tcactgaatc tcaaatattt
     4561 tattagaaaa caccaacaat ttagatattt ctgtatccac ggttggcgtt gacagcctct
     4621 gatggtggag acttgtctgg gcactggcag tactcggcat cctttcctgg tcttccgtct
     4681 ggtccagtct ctccctgggt tccgtctggt ccttgacgtc cttgctttcc agatggtcct
     4741 tctggtccaa cggttcctgg ctctcctggt ggtccgtgaa gaccgttctg tccagctggt
     4801 cctgttggtc cttgatctcc tcttggtccc tttggtcctg gtcttccgat tggatgctcg
     4861 gcgtcgcgtc ccttctctcc tggtggtcct ggctctccga tctttccgat ttgaccggat
     4921 gatccttgct ctcctggatg tcctggttgt ccatctcttc ctgggtttcc attttgtcct
     4981 cttggtcctg gaagtccacg tggtcctggt cttccaagag ctcctggtgg tccaactggt
     5041 ccttgtgggc aagtgaagca tccgacagac tcgcgttgtg gagcgatatc atcagctcca
     5101 actcctggaa caccgtcaag tcctggaact ccgtcgagtc ctggaactcc tggaactccc
     5161 tttggtccgg atggtccagc tgggcacttg ttatcagcat tgcagttgca ggttggtcca
     5221 tttccattgg ctccatttgg agtctttggc tggttgaaga ctggtggaac tgctggtggt
     5281 tgacctggtg gagcagatgg ggatccatca gcatgtggag ttggggtagc gttggtctct
     5341 tcatattgac aacggacacg cttgctagat cttccgatga caactatgtc cttccacaat
     5401 ccattggtag attgctagaa aattaaaaat tatttaaaat tcaaaatata atttaactca
     5461 ctctgaacac attcatctcg gtatcaagct tctctctgaa tccatcaacc tccgagtaga
     5521 tggttgacat ggtcatgaag cataccataa gagtggcgac agtgaccgaa gccgtcacat
     5581 aacacgcaag ttttacagac atctctgaaa ttatcaaatt taggatttca aaataattcg
     5641 gccaaagaaa tgtaactcac ctagtcttcg agaagcagag acttgtcgat ttcctaccgg
     5701 attcgtctcc ttttattagg aaatctttcc gcaagttttc aagttttttt tgtttttttt
     5761 ttacattacc gacgagacag tgtgcgagac gcagagacga taaaagactg gataagaacg
     5821 ggagataagg agtacggatg taggtgagga atgtggccga aaggacagat gggctttatt
     5881 cgggaagatt ggttgggaaa tgaaccagag aagaatgact gaataaagtt gttgaaactt
     5941 ggtgagaatg gaagatagac aatcgagggc agctagtttc cttcctcctc tatatatttt
     6001 tatgttttct tttaaacctt cctccatatt tctgaattga atgttgttgc atattgtgtg
     6061 tccttatttc atttccatca tccttttgtg tgtttccccc ttccaccaat tccccccacg
     6121 tcctaatgaa atgaaagcga gaagataaat aaacttgcac attgtgttca gatgagagga
     6181 gcggaatacg aattgggaga cggaaagaga aaaaaaaatg tttagatttg gattgaatta
     6241 attttttaga aagcaagaaa aaaaacacaa agagaataaa taaataatag gaaaaaaaac
     6301 aaatattcag tatttaaaat ttgaaaattc aacgacgttg gcgtcgatca tccttagagg
     6361 tcctcctcgg agataagctt ttgctctccc tgaaaataac ttcgtataat gtatgctata
     6421 cgaagttatt ttaaacttac ctttccggat tgaaagtaca gattctcctt gtacagctcg
     6481 tccattccca taacatcagt gaaagctttt tgccactcct tgaagtttaa ttcagtttta
     6541 gagtgcttaa gctcggtctt acggaacacg tacattggct ggtttttcaa atagttggca
     6601 gccattggtt tggcgaacgt gtacgttgta cgagctgtcg atcggtatct gaaaatttaa
     6661 atcagattag ttagttagta aaatcatgtt taaacttacc tttttccgtt tccagttgtg
     6721 taagaccatt tgaatgttga aatgatggtc ttgtcatttg gataggtctt cttggagcgg
     6781 caccaatcgg cggcggtgag actgttggtc ataactggtc catcggctgg gaatccagtt
     6841 cccttgactt gagcctcgcc cttgatgtgg gacccctcat aggtataacg atagtttaca
     6901 gtaagggaag ctccgtctga aaatttaaat atgtatggtt agttagtacc gaactgttta
     6961 aacttacctt cgaattgcat tgttcgatga acttggtatc cggatccatc gaccatagcg
     7021 gcttgaaaag gactcattcc atctggatat ggaagatatt gatgaaatcc gtatccaatg
     7081 tggggcacta aaatccatgg tgagaattga aggtctccct gaaaatttaa ataatcaggg
     7141 ttagttagta tatatatgtt taaacttacc ttcgtcgatt ttaagttgag ctcttcgtaa
     7201 ccgtcatttg gatttccagt tccctgtcca accatatcga aatcgactcc gttgatggat
     7261 ccgaaaatgt gaagctcgtg agttgctgga agagaagcca tgttgtcttc ctcgcctttg
     7321 ctgaccatcg atgctcctga ggctcccgat gctccctagg tcgacctgca gactggctgt
     7381 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
     7441 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg
     7501 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     7561 cacgggggac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     7621 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     7681 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcggc aaaagagccg
     7741 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     7801 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccgaacc ggagatattg
     7861 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     7921 gcttcatagc atacctcttt ttgacatact tcgggtaact agtgccggcg actggccgtc
     7981 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8041 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     8101 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     8161 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8221 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8341 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     8401 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8461 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8521 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8581 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8641 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8701 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8761 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8821 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8881 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8941 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9001 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9061 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9121 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9181 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9241 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9301 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9361 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9421 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9481 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9541 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9721 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9901 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10261 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    10321 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    10381 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    10441 gactggaaag cgggcagtga gcgcaacgca at
//
