LOCUS       sequence-274041-        3607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SapTrap HaloTag-GLO donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3607)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD362
FEATURES             Location/Qualifiers
     source          1..3607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1064..1163)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1180..1199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1181..1199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1204..1220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1204..1220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1322..1341)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1333..2142
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1400..1419)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2289..2877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2778..2797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3031..3048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3207..3234)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3326..3412)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3461..3483
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3475..3492
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3476..3492
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3508..3607
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gctcttcgat ggccgaaatc ggaactggat tcccattcga cccccattat gttgaggttc
       61 ttggagaacg catgcattat gttgatgtag gaccacgaga tggaactcca gttctcttcc
      121 ttcacggaaa tccgacttct tcatatgtct ggagaaacat cattccacac gttgctccaa
      181 cacacagatg tattgctcca ggtaagttta ggcatatgtg cacatctgtt catggacacc
      241 atgatttttc agacctcatc ggaatgggaa aatcggataa gccagatctt ggatacttct
      301 tcgatgatca tgttcgattc atggatgcct tcatcgaggc tcttggactc gaagaagtcg
      361 tcctcgtcat acatgattgg ggatctgctc ttggattcca ttgggccaag agaaacccgg
      421 agcgtgtcaa gggaattgct ttcatggagt ttattcgccc aattccaact tgggatgagt
      481 ggcctgaatt cgcccgcgag gtaagtttgc taaagaataa acctgatgta tttcatggtc
      541 cgtattttca gaccttccaa gcattccgga ccaccgatgt cggaagaaag cttatcattg
      601 atcaaaacgt tttcattgaa ggaactcttc cgatgggagt tgtccgtcca cttaccgaag
      661 tcgaaatgga ccactacaga gagccgttcc tcaaccctgt cgacagagaa ccactctgga
      721 ggtttccaaa tgagttgccg atcgctggag gtaagtttct tgctattata ataacacgat
      781 caaataatac tttcttttca gagccggcta acattgttgc tcttgttgag gagtacatgg
      841 actggctgca ccaaagtcca gttccaaaac tccttttctg gggaactcca ggagtcctta
      901 ttccacctgc tgaagccgcc cgtcttgcca agtccctccc aaactgcaag gccgtcgata
      961 tcggaccggg actcaacttg ctccaagaag acaacccaga cctcatcgga tccgaaattg
     1021 cccgctggct ctccaccctt gagatttctg gaaagcgaag agcacccagc tttcttgtac
     1081 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     1141 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     1201 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     1261 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1321 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     1381 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1441 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1501 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1561 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1621 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1681 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1741 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1801 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     1861 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1921 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     1981 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2041 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2101 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2161 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     2221 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2401 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2941 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3001 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3061 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3121 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     3181 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     3241 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     3301 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     3361 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     3421 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     3481 acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc
     3541 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
     3601 gcaggct
//
