LOCUS       sequence-274042-        3409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SapTrap mScarlet-I-GLO donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3409)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMS051
FEATURES             Location/Qualifiers
     source          1..3409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(957..1056)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1073..1092)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1074..1092)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1097..1113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1097..1113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1215..1234)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1226..2035
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1293..1312)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2182..2770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2671..2690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2924..2941
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3100..3127)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3219..3305)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3354..3376
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3368..3385
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3369..3385
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(3401..3409,1..91)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc tgctcttcga tggtctccaa aggagaagct
      121 gtgatcaagg agttcatgcg cttcaaggtt cacatggaag gaagcatgaa tggtcacgag
      181 ttcgaaatcg aaggagaagg ggagggccgc ccgtacgagg gaactcagac cgccaagctg
      241 aaggtcacca agggaggtaa gtttgtgata atccaatttc aattcgcaat ggtcatcgtt
      301 ttttcaggac cacttccatt ctcatgggat attctctccc cacaattcat gtacggctcc
      361 cgtgctttca tcaaacaccc agccgacatt ccagattact acaagcaatc tttcccagaa
      421 ggcttcaagt gggagcgtgt gatgaacttc gaggatggtg gagcagttac agtaactcag
      481 gacacttctc tcgaggtaag tttttactcc gcttttaaca atggttgttt gacatcattt
      541 tttcaggatg gcactttgat ctacaaggtc aagctccgtg gtaccaattt cccaccagat
      601 ggaccagtta tgcagaagaa gacgatggga tgggaggctt ccaccgaacg attgtaccca
      661 gaagatggag ttctcaaggg agacatcaaa atggcccttc gcctcaagga cggaggtaag
      721 tttgatgaaa cggtttcgtc ttatatacac taatggtact tttcaggacg ttacctggcg
      781 gatttcaaga ccacatacaa ggcaaagaag ccagttcaaa tgccaggagc atataacgtt
      841 gaccgcaagc ttgatattac ttcccataat gaagactaca cagttgtaga acaatacgaa
      901 cgatccgagg gacgtcattc gaccggagga atggacgagc tgtacaagcg aagagcaccc
      961 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1021 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1081 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1141 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1201 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     1261 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1321 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     1381 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     1441 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     1501 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     1561 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1621 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     1681 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     1741 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     1801 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     1861 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1921 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1981 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     2041 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     2101 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     2161 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2221 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2281 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2341 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2401 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2461 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2521 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2581 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2641 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2701 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2761 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2821 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2881 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2941 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3001 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3061 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     3121 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     3181 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     3241 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     3301 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
     3361 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatg
//
