LOCUS       sequence-274046-        3433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SapTrap GFP-GLO donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3433)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD373
FEATURES             Location/Qualifiers
     source          1..3433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(990..1089)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1106..1125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1107..1125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1130..1146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1130..1146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1248..1267)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1259..2068
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1326..1345)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2215..2803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2704..2723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2957..2974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3133..3160)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3252..3338)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3387..3409
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3401..3418
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3402..3418
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct gctcttcgat gcgttcttcc
      121 aaaggagaag aactctttac cggagtcgtc ccaattctcg tcgagctcga cggagacgta
      181 aacggacaca aattctcggt ttccggagaa ggagaaggag atgctactta tggaaaactc
      241 acccttaaat tcatttgcac caccggaaag ctcccagtgc cgtgggtaag tttaaacata
      301 tatatactaa ctaaccctga ttatttaaat tttcagccga cccttgtgac tacattctgc
      361 tacggagttc aatgcttttc ccgctaccca gaccacatga agcgtcacga tttcttcaaa
      421 tcagccatgc cagaaggata cgtccaggag cgaacaattt tcttcaagga cgatggaaac
      481 tacaagactc gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
      541 ggtgctgaag tcaagtttga aggagatact ctcgtgaacc gcattgagct caagggaatc
      601 gacttcaaag aagatggaaa cattcttgga cacaagctcg aatacaacta taactcgcac
      661 aacgtgtata tcatggccga caagcaaaaa aatggaatca aggtcgtaag tttaaacatg
      721 attttactaa ctaactaatc tgatttaaat tttcagaact tcaagattcg ccacaacatc
      781 gaagacgggt cggttcaact cgctgatcac taccagcaga acacaccaat tggagatgga
      841 ccagtcctcc tccctgataa tcactacctt tccactcaat ccgctcttag caaggatcca
      901 aatgagaaaa gagatcacat ggttcttctc gagtttgtca ccgccgccgg aatcacccac
      961 ggaatggacg agttgtacaa gcgaagagca cccagctttc ttgtacaaag ttggcattat
     1021 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     1081 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     1141 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1201 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1261 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     1321 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     1381 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1441 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1501 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1561 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1621 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     1681 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     1741 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1801 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     1861 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     1921 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     1981 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     2041 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     2101 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     2161 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2221 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2281 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2341 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2401 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2461 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2521 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2581 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2641 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2701 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2761 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2821 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2881 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2941 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     3001 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     3061 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
     3121 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
     3181 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
     3241 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     3301 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     3361 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
     3421 taagctcggg ccc
//
