LOCUS       sequence-274053-        6815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 
            without its STOP codon .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Marchal C, Wheat Genome Project, Haberer G, Spannagl M, Uauy C
  TITLE     Comparative Genomics and Functional Studies of Wheat BED-NLR Loci.
  JOURNAL   Genes (Basel). 2020 Nov 26;11(12). pii: genes11121406. doi: 
            10.3390/genes11121406.
  PUBMED    33256067
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2020-11-26"; volume: "11(12). pii: genes11121406. 
            doi"; pages: " 10.3390/genes11121406"
COMMENT     Sequence Label: pUAP1_Ta_Yr7_CDS_no_STOP
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      7..64
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(88..105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(259..847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(339..358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1030..1124)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1145..1804)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1719..1738
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1966..1984
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1988..2031)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
       61 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
      121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      241 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
      301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      661 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      961 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     1021 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1081 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1141 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     1201 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     1261 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1321 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     1381 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1441 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1501 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1561 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     1621 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1681 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1741 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1801 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     1861 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
     1921 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     1981 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
     2041 gtctcaaatg gagccggcgg gagactcttc cctggaggcc gcgattgcat ggctggtgca
     2101 gaccatcctt gcaacgctcc tcatggacaa gatggaggcc tggattcagc aagtcgggct
     2161 tgccgacgac gtcgagaggc tccagtctga ggtcgagaga gtcgacacgg tggtggctgc
     2221 tgtgaagggg agggcagccg ggaacatgcc tctgtcccgg tcactcgctc gtgtcaagga
     2281 gcttctctat gacgccgacg acgtgatcga cgagctagac tactacaggc tccaacacca
     2341 agtcgaagga gttacaagtg acgagcctga cggtatgcgt ggagctgaaa gagtggatga
     2401 aatatcaagg ggccatgtcg atacactgaa tgtcagtgtt ggcaaattac ggtccccggt
     2461 atgggaacac ttcaccatca cagaaacaac tatcgacggg aagcgttcaa aagccaaatg
     2521 taagtactgt ggaaatgatt ttaattgcga aacgaaaaca aacgggactt catctatgaa
     2581 aaaacatttg gagaaggagc attccgtgac ttgcacgaat aaatctgcag tgcacccccc
     2641 aaacacttca agcaccggcg atgctacttg taatgtgagg tcggttgaag ttggtagttc
     2701 gtccaacgga aaaagaaaga gaacaaatga ggatccaacg cagaccaccg cagctaacat
     2761 acacgcccaa tgggacaagg ctgagttatc caataggata attaaaatta ctgagaagtt
     2821 acagttacag gacatccagg gggctttgag taaagttctc gagccatatg gatccagcgc
     2881 tacttcaagt tcaaatcatc accgcttgag tacagcatca gatcagcacc caacaacatc
     2941 aagtcttgtt ccaatggaag tttatggcag agttgcagaa aagaataaga tcaaaaagtc
     3001 aataactgaa aaccaatctg gtggtgtaaa tgttctgcct attgtaggca ttgcaggtgt
     3061 tggaaagaca actcttgctc aatttgtgta taatgatcca gacgtgaaaa gtcaatttca
     3121 ccacaggata tgggtttgtg tgtcctgcaa atttgatgaa gtgaagctca caaaggagat
     3181 gttagacttt tttcctcgag aaaggcatga aggaattaac aacttcgcga agcttcaaga
     3241 gatcttgaaa gaacatgtcg agtaccaagc aaagagtttt ctgctcattt tagatgatgt
     3301 ctcggacagt atggattatc ataaatggaa caaattgttg aaccctttgc tatcaagtca
     3361 agcgaagaat ataattctag tcacgaccag aaatttgtct gttgcacaaa ggttaagcac
     3421 acttgaaccg atcaagttag gtgctttaga aaacgatgat atgtggttat tgctcaagtc
     3481 atgtgcattt ggttttggga actatgaagg tacggaaaat ctaagcacta ttggaaggca
     3541 aatagcagag aagttaaagg gcaatccgtt agcagcagta actgcagggg cactgttaag
     3601 agataatctt agcattgatc attggagtaa cattctcaag aatgagaagt ggaaatcgct
     3661 gggactcagt gggggcatca tgcctgcttt gaagcttagt tatgatgagt tgacgtaccg
     3721 tttacaacaa tgtttctcgt attgctctat atttcctgac aaatataggt ttctcgggaa
     3781 ggatttggtc tatatttgga tttctcaggg atttgtgaat tgcacccaaa ataagagatt
     3841 ggaggagacg ggatgggaat atctgaatca attggtaaat cttggattct ttcaacaaat
     3901 tgaagaacaa caagaattgg atggggaaga agaattctct ctacgccgtc agatttggta
     3961 ctctatgtgt gatctcatgc atgatttcgc aaggatgatt tcaaggactg aatgtgcgac
     4021 tatagatggt ctacagtgca ataaaatatt cccaactgta cagcatttgt caatagtaac
     4081 cggttctgca tacaacaaag atctgaaggg gaacattcct cgtaatgaga agtttgaaga
     4141 aaatatgaga aattcagtta catcagttac caaattgaga acattggttg tgcttgggaa
     4201 ctttgactct ttctttgtac ggttgttcca agatatattc cagaaggcac aaaatttacg
     4261 cctgctgcta gtatctctag catccactta tctgtctcaa gtgcctgctg cattcaatga
     4321 ttttaattcc ttcctgtgca atttggcaaa tcctttgcat cttcgttacc taaaacttga
     4381 gttggatggg attgtgccac aagttttgag tacgtttttt catcttcaag tattagatgt
     4441 tggatcaagc atggatactt ctctacccaa tggcttgttg cataatcttg ttagcctgcg
     4501 acatcttgtt gcacacaaga gagtccattc ttccattact agcattggta acatgacatc
     4561 tatccaggag ctacatgatt ttgaagttcg aatttctagc ggctttgaga taacacgact
     4621 ccaatccatg aacgagcttg ttcaacttgg gttgtctcaa cttgacagtg ttaaaaccag
     4681 ggaggacgct tatggggcag gactaagaaa caaggaacac ttagaagagc ttcatttgtc
     4741 ctggaaggat gcatattcag agtatgagta tgccagtgac actgaatttg aatcttctgc
     4801 aaacatggca agagaagtga ttgagggtct tgaaccacac atggatttaa aacatctaca
     4861 aatatctcag tataatggta ccacttcacc agcttggctt gccaacaata tctcagttac
     4921 ctcattgcag acgcttcatc ttgatgattg tggaggatgg agaatacttc catctctggg
     4981 aagtctacca ttccttacaa aggtgaagtt gagcagcatg ctggaagtaa ttgaagtact
     5041 gattccttca ctggaggagc tagttctaat taaaatgccg aagttagtga gatgctcaag
     5101 cacttctgcc gagggtctga gctctagctt aagggtactg cacattgagg attgtgaagc
     5161 attgaaggag tttgatctgt ttgagaacga ttataattct gaaatcattc agggatcatg
     5221 gctgcctggt cttaggaatt tgattctata ttgttgccct catttgaaag tgttgaagcc
     5281 tcttccacct tcaactacct tttctaaggt actcatcaga gaaatttcaa gatttccgtc
     5341 tatggaggta tcatctggtg agaagttaca aattgggaat attgatgtgt acataggcga
     5401 tgattttgat gagtcatctg atgagttgag catactggat gacaaaactt tggcgttcca
     5461 taatcttaga aacctgaaat cgatggagat atatggttgc agaaatctaa ggtctttttc
     5521 gttcgaaggt ttcagtcatc ttgtctcttt aacaagtttg aaaatagtaa gctgtgaaca
     5581 acttttccct tcagatgtga cggcagagta tacccttgaa gatgtgacag ctgtgaactg
     5641 caatgccttc ccatatctta aaagcctcag tatcgactca tgtggaatag cggggaagtg
     5701 gctatcgctg atgctgcagc atgcgccagg cctagaggaa ttgagtttaa caagttgcgc
     5761 ccatataaca agagtagtgt taccgatgga agaggaagaa aacaatctat taacaacagt
     5821 actgtcatca ggaaatcaag atgaggcatt gacatggtta gttcgtgacg gactcttgca
     5881 cattccatca aatctcgtct cctctctcaa gaatatgagt attactcagt gccctcgcct
     5941 aaagtttaac tcaggcaagg actgcttctc tggatttacc tcgcttgaga agcttgaaat
     6001 ttggggatcg ttggtggatg atgacggaag tgatgacctg gagaatggaa gttcttttgt
     6061 gttcggagag gaggatcaac ccctgggggc gaacggaaga tggctcctcc cgacatcact
     6121 tcaggaactt cacatcgtgt cattgtattg ccaagaaacg ctgcaagtct gcttccctag
     6181 agatatcacc agccttaaaa agttaagtgt acgttccggc caaggtttgc aatctctaca
     6241 gctgtactca tgcacggcac tggaagaatt ggcaatttcc ggctctggat cggtcaccgt
     6301 cactgtacta gagggcacgc aacccgctgg cagcctcggg cgtttgaatg tatcagactg
     6361 tcctggcttg ccatcacgtt tggacagctt tccaaggttg tgccctcggc tggaaaggct
     6421 tgacatcaat gacccatctg tccttaccac gccattctgc aagcacctca cctccctgca
     6481 acgcctaaaa cttggcttct tgaaagtgac gagactaaca gatgagcaag aacgagcgct
     6541 tgtgctcctc aagtcactga aagagctcga gattttttat tgtactcatc tcatagatct
     6601 tcctgcgggg ctgcagaccc ttccttccct caagagtttg aagatagaag agggtcgagg
     6661 catctcaagg ctgccggaag caggcctccc acattcgctg gaagaactgg aaatcaaaat
     6721 ttgcagcaag ctagaagatg aatgcaggcg gctagcaaca tgcgaaggca agctaaaagt
     6781 caaaattgat ggtcgatatg tgaattcgcg agacc
//
