LOCUS       sequence-274056-        8439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 1x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-F-bpA-SV40pA-F-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        157..536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        538..815
                     /label=chicken beta-actin promoter
     intron          816..1832
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1756..1778
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1840..1859
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1892..1925)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    1946..2170
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2355..2476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2392..2411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2446..2465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(2494..2527)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             join(2537..2539,2540..3250)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2537..3250
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3281..3283,3284..3997)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3281..3994
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4055..4075)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4473..4484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4635..4859
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5213..5247
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5334..5353)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5335..5353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5360..5377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5360..5376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5369..5391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5518..5973)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5655..5676)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5867..5886
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6000..6104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6105..6173,6174..6965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6323..6342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7136..7724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7625..7644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7878..7895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8012..8033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8048..8053,8054..8071,8072..8078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8086..8102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8091..8113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8110..8126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8110..8126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8145..8165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8147..8165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8229..8291
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ttcttctata aagtaacaaa acttttaaac attctctctt ttacaaaaat aaacttattt
       61 tgtactttaa aaacagtcat gttgtattat aaaataagta attagcttaa cctatacata
      121 atagaaacaa attatactta ttaaccggtc atggtcgaca ttgattattg actagttatt
      181 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      241 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      301 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      361 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      421 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      481 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
      541 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      601 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc
      661 gcgcgccggg gggggggggg gggggggggg gggggggsgg ggsgrggcgg agaggtgcgg
      721 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      781 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
      841 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
      901 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
      961 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     1021 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     1081 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     1141 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     1201 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     1261 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     1321 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1381 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggrgggct
     1441 cgggggrggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1501 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1561 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     1621 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1681 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     1741 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     1801 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     1861 tgtctcatca ttttggcaaa gaattgctag cgaagttcct atactttcta gagaatagga
     1921 acttcagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     1981 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     2041 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2101 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2161 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgactagagc ttgcggaacc
     2221 cttaccaccg cggtggcggc cgctctagaa tcataatcag ccataccaca tttgtagagg
     2281 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2341 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2461 tcatcaatgt atcttataac taaataacct agggaagttc ctatactttc tagagaatag
     2521 gaacttcagg cgcgccatga gcaaaggaga agaacttttc actggagttg tcccaattct
     2581 tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg
     2641 tgatgctaca aacggaaaac tcacccttaa atttatttgc actactggaa aactacctgt
     2701 tccgtggcca acacttgtca ctactctgac ctatggtgtt caatgctttt cccgttatcc
     2761 ggatcacatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     2821 acgcactata tctttcaaag atgacgggac ctacaagacg cgtgctgaag tcaagtttga
     2881 aggtgatacc cttgttaatc gtatcgagtt aaagggtatt gattttaaag aagatggaaa
     2941 cattcttgga cacaaactcg aatacaactt taactcacac aatgtataca tcacggcaga
     3001 caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc
     3061 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     3121 accagacaac cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg
     3181 tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga
     3241 gctctacaaa ggttctgctg gatctgcagc aggatctggt atgagcaaag gagaagaact
     3301 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     3361 ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga aaactcaccc ttaaatttat
     3421 ttgcactact ggaaaactac ctgttccgtg gccaacactt gtcactactc tgacctatgg
     3481 tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc
     3541 catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg ggacctacaa
     3601 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg
     3661 tattgatttt aaagaagatg gaaacattct tggacacaaa ctcgaataca actttaactc
     3721 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
     3781 tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc
     3841 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct
     3901 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
     3961 tgggattaca catggcatgg atgagctcta caaatgatta attaaaacct ctggattaca
     4021 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4081 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4141 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4201 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4261 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4321 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4381 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4441 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4501 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4561 gtcggatctc cctttgggcc gcctccccgc aggatccact agagcggccg cctagagctc
     4621 gctatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4681 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4741 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4801 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4861 ttctgaggcg gaaagaacca gctggggctc gactagagct tgcggaaccc ttaagctttt
     4921 ttattatata ttagtcacga tatctataac aagaaaatat atatataata agttatcacg
     4981 taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta
     5041 aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact
     5101 taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg
     5161 cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc
     5221 aattttacgc agactatctt tctagggtta aaaaagattt gcgctttact cgacctaaac
     5281 tttaaacacg tcatagaatc ttcgtttgac aaaaaccaca ttgtgttggg tcgccctata
     5341 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5401 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     5461 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcaaa
     5521 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5581 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5641 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5701 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5761 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5821 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5881 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5941 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     6001 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6061 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6121 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6181 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6241 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6301 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6361 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6421 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6481 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6541 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6601 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6661 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6721 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6781 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6841 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6901 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6961 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7021 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7801 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7861 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7921 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7981 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8041 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8101 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8161 aaagggaacc tcccccggta ttcacgacag caggctgaat aataaaaaaa ttagaaacta
     8221 ttatttaacc ctagaaagat aatcatattg tgacgtacgt taaagataat catgcgtaaa
     8281 attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata
     8341 ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac aatttattta
     8401 tgtttattta tttattaaaa aaaaacaaaa actcaaaat
//
