LOCUS       sequence-274057-        8704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-F-bpA-2xSV40pA-F-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..850
                     /label=chicken beta-actin promoter
     intron          851..1867
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1791..1813
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1875..1894
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1927..1960)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    1981..2205
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2390..2511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2623..2744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2762..2795)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             join(2805..2807,2808..3518)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2805..3518
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3549..3551,3552..4265)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3549..4262
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    4302..4890
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4355..4375)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4773..4784)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4935..5159
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5513..5547
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5634..5653)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5635..5653)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5660..5677)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5660..5676)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5669..5691)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5818..6273)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5955..5976)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6167..6186
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6300..6404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6405..6473,6474..7265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6623..6642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7436..8024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7925..7944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8178..8195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8312..8333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8348..8353,8354..8371,8372..8378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8386..8402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8391..8413
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8410..8426
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8410..8426
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8445..8465
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8447..8465
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8529..8591
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tatttattta ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta acaaaacttt
       61 taaacattct ctcttttaca aaaataaact tattttgtac tttaaaaaca gtcatgttgt
      121 attataaaat aagtaattag cttaacctat acataataga aacaaattat acttattaac
      181 cggtcatggt cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
      601 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
      661 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccgggggggg gggggggggg
      721 gggggggggg ggsggggsgr ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
      781 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
      841 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
      901 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
      961 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     1021 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     1081 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     1141 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     1201 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     1261 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     1321 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1381 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1441 gggcggggcg gggccgcckc gggccggggr gggctcgggg grggggcgcg gcggcccccg
     1501 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1561 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1621 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1681 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     1741 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     1801 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     1861 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1921 gctagcgaag ttcctatact ttctagagaa taggaacttc agctcgctga tcagcctcga
     1981 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2041 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2101 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     2161 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     2221 gaaccagctg gggctcgact agagcttgcg gaacccttac caccgcggtg gcggccgctc
     2281 tagaatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2341 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2401 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2461 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aactagaatc
     2521 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     2581 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     2641 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2701 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttataacta aataacctag
     2761 ggaagttcct atactttcta gagaatagga acttcaggcg cgccatgagc aaaggagaag
     2821 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     2881 aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat
     2941 ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact actctgacct
     3001 atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga
     3061 gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct
     3121 acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa
     3181 agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgaa tacaacttta
     3241 actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca
     3301 aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata
     3361 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg
     3421 tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg
     3481 ctgctgggat tacacatggc atggatgagc tctacaaagg ttctgctgga tctgcagcag
     3541 gatctggtat gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     3601 tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta
     3661 caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc
     3721 caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat ccggatcaca
     3781 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta
     3841 tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata
     3901 cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga aacattcttg
     3961 gacacaaact cgaatacaac tttaactcac acaatgtata catcacggca gacaaacaaa
     4021 agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac
     4081 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     4141 accattacct gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca
     4201 tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca
     4261 aatgattaat taatgatcat acagtgctct gagtacgcta taatcaacct ctggattaca
     4321 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4381 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4441 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4501 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4561 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4621 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4681 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4741 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4801 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4861 gtcggatctc cctttgggcc gcctccccgc aggatccact agagcggccg cctagagctc
     4921 gctatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4981 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5041 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5101 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     5161 ttctgaggcg gaaagaacca gctggggctc gactagagct tgcggaaccc ttaagctttt
     5221 ttattatata ttagtcacga tatctataac aagaaaatat atatataata agttatcacg
     5281 taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta
     5341 aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact
     5401 taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg
     5461 cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc
     5521 aattttacgc agactatctt tctagggtta aaaaagattt gcgctttact cgacctaaac
     5581 tttaaacacg tcatagaatc ttcgtttgac aaaaaccaca ttgtgttggg tcgccctata
     5641 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5701 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     5761 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcaaa
     5821 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5881 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5941 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     6001 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     6061 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     6121 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     6181 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     6241 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     6301 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6361 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6421 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6481 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6541 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6601 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6661 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6721 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6781 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6841 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6901 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6961 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7021 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7081 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7141 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7201 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7261 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7321 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7381 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7441 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7501 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7561 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7621 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7681 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7741 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7801 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7861 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7921 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7981 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8041 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8101 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8161 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8221 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8281 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8341 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8401 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8461 aaagggaacc tcccccggta ttcacgacag caggctgaat aataaaaaaa ttagaaacta
     8521 ttatttaacc ctagaaagat aatcatattg tgacgtacgt taaagataat catgcgtaaa
     8581 attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata
     8641 ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac aatttattta
     8701 tgtt
//
