LOCUS       sequence-274063-        8910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-F-bpA-3xSV40pA-F-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        150..529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..808
                     /label=chicken beta-actin promoter
     intron          809..1825
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1749..1771
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1833..1852
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1880..1913)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    1940..2164
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2349..2470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2582..2703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2815..2936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2959..2992)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             join(3001..3003,3004..3714)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3001..3714
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3745..3747,3748..4461)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3745..4458
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4519..4539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4937..4948)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5099..5323
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5677..5711
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5798..5817)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5799..5817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5824..5841)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5824..5840)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5833..5855)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5982..6437)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6119..6140)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6331..6350
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6464..6568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6569..6637,6638..7429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6787..6806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7600..8188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8089..8108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8342..8359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8476..8497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8512..8517,8518..8535,8536..8542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8550..8566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8555..8577
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8574..8590
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8574..8590
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8609..8629
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8611..8629
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8693..8755
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ataaagtaac aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt
       61 taaaaacagt catgttgtat tataaaataa gtaattagct taacctatac ataatagaaa
      121 caaattatac ttattaaccg gtcatggtcg acattgatta ttgactagtt attaatagta
      181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
      541 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
      601 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggsgcgcgcc
      661 gggggggggg gggggggggg gggggggggg gggggsgrgg cggagaggtg cggcggcagc
      721 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
      781 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
      841 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
      901 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
      961 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1021 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     1081 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     1141 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     1201 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1261 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     1321 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     1381 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggrgg gctcggggga
     1441 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1501 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     1561 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     1621 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     1681 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     1741 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     1801 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     1861 tcattttggc aaagaattcg aagttcctat actttctaga gaataggaac ttcgctagca
     1921 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     1981 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2041 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2101 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2161 atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcttgcgg aacccttacc
     2221 accgcggtgg cggccgctct agaatcataa tcagccatac cacatttgta gaggttttac
     2281 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     2341 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2401 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2461 atgtatctta actagaatca taatcagcca taccacattt gtagaggttt tacttgcttt
     2521 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     2581 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2641 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2701 ttaactagaa tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2761 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2821 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2881 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacta
     2941 gtggatctca tgcctaggga agttcctata ctttctagag aataggaact tcggcgcgcc
     3001 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     3061 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga
     3121 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
     3181 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg
     3241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
     3301 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
     3361 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa
     3421 ctcgaataca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
     3481 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac
     3541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     3601 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     3661 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaggttct
     3721 gctggatctg cagcaggatc tggtatgagc aaaggagaag aacttttcac tggagttgtc
     3781 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt ccgtggagag
     3841 ggtgaaggtg atgctacaaa cggaaaactc acccttaaat ttatttgcac tactggaaaa
     3901 ctacctgttc cgtggccaac acttgtcact actctgacct atggtgttca atgcttttcc
     3961 cgttatccgg atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
     4021 gtacaggaac gcactatatc tttcaaagat gacgggacct acaagacgcg tgctgaagtc
     4081 aagtttgaag gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa
     4141 gatggaaaca ttcttggaca caaactcgaa tacaacttta actcacacaa tgtatacatc
     4201 acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacgttgaa
     4261 gatggttccg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     4321 gtccttttac cagacaacca ttacctgtcg acacaatctg tcctttcgaa agatcccaac
     4381 gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc
     4441 atggatgagc tctacaaatg attaattaaa acctctggat tacaaaattt gtgaaagatt
     4501 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4561 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4621 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4681 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4741 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4801 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4861 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4921 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4981 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5041 ggccgcctcc ccgcaggatc cactagagcg gccgcctaga gctcgctatc agcctcgact
     5101 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5161 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5221 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5281 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5341 accagctggg gctcgactag agcttgcgga acccttaagc ttttttatta tatattagtc
     5401 acgatatcta taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa
     5461 taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt
     5521 cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc
     5581 acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg
     5641 cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta
     5701 tctttctagg gttaaaaaag atttgcgctt tactcgacct aaactttaaa cacgtcatag
     5761 aatcttcgtt tgacaaaaac cacattgtgt tgggtcgccc tatagtgagt cgtattacaa
     5821 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5881 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5941 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat
     6001 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     6061 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     6121 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     6181 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     6241 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     6301 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     6361 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     6421 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6901 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6961 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7021 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7081 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7141 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7201 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7261 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7321 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7381 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7441 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7501 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7561 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7741 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8221 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8281 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8341 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8401 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8461 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     8521 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     8581 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacctccccc
     8641 ggtattcacg acagcaggct gaataataaa aaaattagaa actattattt aaccctagaa
     8701 agataatcat attgtgacgt acgttaaaga taatcatgcg taaaattgac gcatgtgttt
     8761 tatcggtctg tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat
     8821 ttacacttac atactaataa taaattcaac aaacaattta tttatgttta tttatttatt
     8881 aaaaaaaaac aaaaactcaa aatttcttct
//
