LOCUS       sequence-274064-        8613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP cre/lox reporter piggyBac transposon (3x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-L-3xSV40pA-L-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        128..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        509..786
                     /label=chicken beta-actin promoter
     intron          787..1803
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1727..1749
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1811..1830
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1863..1896)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2029..2150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2262..2383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2495..2616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2639..2672)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(2682..2684,2685..3395)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2682..3395
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3426..3428,3429..4142)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3426..4139
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4200..4220)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4618..4629)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4774..4791)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4780..5004
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5358..5392
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5479..5498)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5480..5498)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5505..5522)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5505..5521)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5514..5536)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5663..6118)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5800..5821)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6012..6031
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6145..6249
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6250..6318,6319..7110)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6468..6487)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7281..7869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7770..7789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8023..8040
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8157..8178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8193..8198,8199..8216,8217..8223)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8231..8247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8236..8258
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8255..8271
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8255..8271
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8290..8310
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8292..8310
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8374..8436
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta
       61 taaaataagt aattagctta acctatacat aatagaaaca aattatactt attaaccggt
      121 catggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
      541 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      601 gcagcgatgg gggcgggggg gggggggggg cgcgcgccgg gggggggggg gggggggggg
      661 ggggggggsg gggsgrggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
      721 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      781 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      841 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      901 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
      961 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     1021 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     1081 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     1141 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     1201 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     1261 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     1321 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     1381 ggggcggggc cgcctcgggc cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc
     1441 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     1501 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     1561 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     1621 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     1681 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     1741 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     1801 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgcta
     1861 gcataacttc gtatagcata cattatacga agttatagcc accgcggtgg cggccgctct
     1921 agaatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1981 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2041 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2101 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta actagaatca
     2161 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2221 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2281 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2341 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaactagaa tcataatcag
     2401 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2461 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2521 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2581 tagttgtggt ttgtccaaac tcatcaatgt atcttaacta gtggatctca tgcctaggat
     2641 aacttcgtat agcatacatt atacgaagtt ataggcgcgc catgagcaaa ggagaagaac
     2701 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     2761 tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc cttaaattta
     2821 tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact ctgacctatg
     2881 gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt ttcaagagtg
     2941 ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac gggacctaca
     3001 agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaagg
     3061 gtattgattt taaagaagat ggaaacattc ttggacacaa actcgaatac aactttaact
     3121 cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa
     3181 ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa caaaatactc
     3241 caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgtcc
     3301 tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg
     3361 ctgggattac acatggcatg gatgagctct acaaaggttc tgctggatct gcagcaggat
     3421 ctggtatgag caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     3481 atggtgatgt taatgggcac aaattttctg tccgtggaga gggtgaaggt gatgctacaa
     3541 acggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt ccgtggccaa
     3601 cacttgtcac tactctgacc tatggtgttc aatgcttttc ccgttatccg gatcacatga
     3661 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat
     3721 ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt caagtttgaa ggtgataccc
     3781 ttgttaatcg tatcgagtta aagggtattg attttaaaga agatggaaac attcttggac
     3841 acaaactcga atacaacttt aactcacaca atgtatacat cacggcagac aaacaaaaga
     3901 atggaatcaa agctaacttc aaaattcgcc acaacgttga agatggttcc gttcaactag
     3961 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     4021 attacctgtc gacacaatct gtcctttcga aagatcccaa cgaaaagcgt gaccacatgg
     4081 tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaat
     4141 gattaattaa aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4201 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4261 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4321 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4381 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4441 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4501 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4561 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4621 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4681 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcaggat
     4741 ccactagagc ggccgcctag agctcgctat cagcctcgac tgtgccttct agttgccagc
     4801 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4861 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4921 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4981 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacta
     5041 gagcttgcgg aacccttaag cttttttatt atatattagt cacgatatct ataacaagaa
     5101 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
     5161 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
     5221 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
     5281 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
     5341 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaaaaaa
     5401 gatttgcgct ttactcgacc taaactttaa acacgtcata gaatcttcgt ttgacaaaaa
     5461 ccacattgtg ttgggtcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
     5521 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5581 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5641 cgcagcctga atggcgaatg gcaaattgta agcgttaata ttttgttaaa attcgcgtta
     5701 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5761 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5821 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5881 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5941 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     6001 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     6061 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     6121 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6181 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6241 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6301 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6361 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6421 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6481 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6541 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6601 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6661 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6721 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6781 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6841 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6901 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6961 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7021 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7081 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7141 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7201 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7261 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7321 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7381 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7441 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7501 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7561 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7621 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7681 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7741 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7801 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7861 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7921 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7981 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8041 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8101 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     8161 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     8221 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     8281 gccaagcgcg caattaaccc tcactaaagg gaacctcccc cggtattcac gacagcaggc
     8341 tgaataataa aaaaattaga aactattatt taaccctaga aagataatca tattgtgacg
     8401 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
     8461 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     8521 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     8581 aaatttcttc tataaagtaa caaaactttt aaa
//
