LOCUS       sequence-274071-        8704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-L-bpA-2xSV40pA-L-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        166..545
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        547..824
                     /label=chicken beta-actin promoter
     intron          825..1841
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1765..1787
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1849..1868
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1901..1934)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1955..2179
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2364..2485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2597..2718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2736..2769)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(2779..2781,2782..3492)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2779..3492
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3523..3525,3526..4239)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3523..4236
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    4276..4864
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4329..4349)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4747..4758)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4909..5133
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5487..5521
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5608..5627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5609..5627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5634..5651)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5634..5650)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5643..5665)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5792..6247)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5929..5950)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6141..6160
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6274..6378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6379..6447,6448..7239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6597..6616)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7410..7998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7899..7918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8152..8169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8286..8307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8322..8327,8328..8345,8346..8352)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8360..8376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8365..8387
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8384..8400
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8384..8400
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8419..8439
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8421..8439
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8503..8565
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 actcaaaatt tcttctataa agtaacaaaa cttttaaaca ttctctcttt tacaaaaata
       61 aacttatttt gtactttaaa aacagtcatg ttgtattata aaataagtaa ttagcttaac
      121 ctatacataa tagaaacaaa ttatacttat taaccggtca tggtcgacat tgattattga
      181 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      241 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      301 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      361 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      421 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      481 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      541 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      601 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      661 ggggggggcg cgcgccgggg gggggggggg ggggsggggg gsggggsggg gsgrggcgga
      721 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      781 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      841 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      901 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      961 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
     1021 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
     1081 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
     1141 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     1201 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     1261 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     1321 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     1381 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cckcgggccg
     1441 gggrgggctc gggggrgggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     1501 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1561 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1621 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1681 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     1741 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     1801 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     1861 ttattgtgct gtctcatcat tttggcaaag aattgctagc ataacttcgt atagcataca
     1921 ttatacgaag ttatagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     1981 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2041 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2101 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2161 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gactagagct
     2221 tgcggaaccc ttaccaccgc ggtggcggcc gctctagaat cataatcagc cataccacat
     2281 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2341 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2401 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2461 tgtccaaact catcaatgta tcttaactag aatcataatc agccatacca catttgtaga
     2521 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2581 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2641 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2701 actcatcaat gtatcttata actaaataac ctaggataac ttcgtatagc atacattata
     2761 cgaagttata ggcgcgccat gagcaaagga gaagaacttt tcactggagt tgtcccaatt
     2821 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa
     2881 ggtgatgcta caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct
     2941 gttccgtggc caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat
     3001 ccggatcaca tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     3061 gaacgcacta tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt
     3121 gaaggtgata cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga
     3181 aacattcttg gacacaaact cgaatacaac tttaactcac acaatgtata catcacggca
     3241 gacaaacaaa agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt
     3301 tccgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     3361 ttaccagaca accattacct gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag
     3421 cgtgaccaca tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat
     3481 gagctctaca aaggttctgc tggatctgca gcaggatctg gtatgagcaa aggagaagaa
     3541 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     3601 ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg gaaaactcac ccttaaattt
     3661 atttgcacta ctggaaaact acctgttccg tggccaacac ttgtcactac tctgacctat
     3721 ggtgttcaat gcttttcccg ttatccggat cacatgaaac ggcatgactt tttcaagagt
     3781 gccatgcccg aaggttatgt acaggaacgc actatatctt tcaaagatga cgggacctac
     3841 aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat cgagttaaag
     3901 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aactcgaata caactttaac
     3961 tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa
     4021 attcgccaca acgttgaaga tggttccgtt caactagcag accattatca acaaaatact
     4081 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtcgac acaatctgtc
     4141 ctttcgaaag atcccaacga aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct
     4201 gctgggatta cacatggcat ggatgagctc tacaaatgat taattaatga tcatacagtg
     4261 ctctgagtac gctataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4321 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4381 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4441 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4501 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4561 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4621 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4681 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4741 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4801 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4861 ccgcaggatc cactagagcg gccgcctaga gctcgctatc agcctcgact gtgccttcta
     4921 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4981 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5041 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5101 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     5161 gctcgactag agcttgcgga acccttaagc ttttttatta tatattagtc acgatatcta
     5221 taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata
     5281 attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact
     5341 cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg
     5401 ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
     5461 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
     5521 gttaaaaaag atttgcgctt tactcgacct aaactttaaa cacgtcatag aatcttcgtt
     5581 tgacaaaaac cacattgtgt tgggtcgccc tatagtgagt cgtattacaa ttcactggcc
     5641 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5701 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5761 caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat tttgttaaaa
     5821 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     5881 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     5941 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     6001 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     6061 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     6121 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     6181 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     6241 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6301 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6361 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6421 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6481 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6541 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6601 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6661 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6721 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6781 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6841 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6901 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6961 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7021 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7081 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7141 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7201 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7261 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7321 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7981 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8041 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8101 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8161 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8221 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8281 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8341 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8401 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacctccccc ggtattcacg
     8461 acagcaggct gaataataaa aaaattagaa actattattt aaccctagaa agataatcat
     8521 attgtgacgt acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg
     8581 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     8641 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac
     8701 aaaa
//
