LOCUS       sequence-274079-        8911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-L-bpA-3xSV40pA-L-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          764..1780
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1704..1726
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1788..1807
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1835..1868)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1895..2119
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2304..2425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2537..2658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2770..2891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2914..2947)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(2957..2959,2960..3670)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2957..3670
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3701..3703,3704..4417)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3701..4414
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4475..4495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4893..4904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5055..5279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5633..5667
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5754..5773)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5755..5773)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5780..5797)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5780..5796)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5789..5811)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5938..6393)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6075..6096)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6287..6306
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6420..6524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6525..6593,6594..7385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6743..6762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7556..8144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8045..8064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8298..8315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8432..8453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8468..8473,8474..8491,8492..8498)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8506..8522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8511..8533
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8530..8546
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8530..8546
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8565..8585
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8567..8585
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8649..8711
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 acttattttg tactttaaaa acagtcatgt tgtattataa aataagtaat tagcttaacc
       61 tatacataat agaaacaaat tatacttatt aaccggtcat ggtcgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      541 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      601 gggggggsgc gcgccggggg gggggggggg gggggggggg gggggggggg sgrggcggrg
      661 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      721 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
      781 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
      841 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
      901 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
      961 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     1021 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     1081 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     1141 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     1201 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     1261 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     1321 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ckcgggccgg
     1381 ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     1441 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     1501 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     1561 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     1621 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     1681 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1741 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1801 tattgtgctg tctcatcatt ttggcaaaga attcataact tcgtatagca tacattatac
     1861 gaagttatgc tagcagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     1921 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1981 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2041 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2101 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gactagagct
     2161 tgcggaaccc ttaccaccgc ggtggcggcc gctctagaat cataatcagc cataccacat
     2221 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2281 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2341 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2401 tgtccaaact catcaatgta tcttaactag aatcataatc agccatacca catttgtaga
     2461 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2521 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2581 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2641 actcatcaat gtatcttaac tagaatcata atcagccata ccacatttgt agaggtttta
     2701 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2761 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2821 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2881 aatgtatctt aactagtgga tctcatgcct aggataactt cgtatagcat acattatacg
     2941 aagttatagg cgcgccatga gcaaaggaga agaacttttc actggagttg tcccaattct
     3001 tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg
     3061 tgatgctaca aacggaaaac tcacccttaa atttatttgc actactggaa aactacctgt
     3121 tccgtggcca acacttgtca ctactctgac ctatggtgtt caatgctttt cccgttatcc
     3181 ggatcacatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     3241 acgcactata tctttcaaag atgacgggac ctacaagacg cgtgctgaag tcaagtttga
     3301 aggtgatacc cttgttaatc gtatcgagtt aaagggtatt gattttaaag aagatggaaa
     3361 cattcttgga cacaaactcg aatacaactt taactcacac aatgtataca tcacggcaga
     3421 caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc
     3481 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     3541 accagacaac cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg
     3601 tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga
     3661 gctctacaaa ggttctgctg gatctgcagc aggatctggt atgagcaaag gagaagaact
     3721 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     3781 ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga aaactcaccc ttaaatttat
     3841 ttgcactact ggaaaactac ctgttccgtg gccaacactt gtcactactc tgacctatgg
     3901 tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc
     3961 catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg ggacctacaa
     4021 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg
     4081 tattgatttt aaagaagatg gaaacattct tggacacaaa ctcgaataca actttaactc
     4141 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
     4201 tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc
     4261 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct
     4321 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
     4381 tgggattaca catggcatgg atgagctcta caaatgatta attaaaacct ctggattaca
     4441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4801 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     4861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4981 gtcggatctc cctttgggcc gcctccccgc aggatccact agagcggccg cctagagctc
     5041 gctatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5101 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5161 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5221 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     5281 ttctgaggcg gaaagaacca gctggggctc gactagagct tgcggaaccc ttaagctttt
     5341 ttattatata ttagtcacga tatctataac aagaaaatat atatataata agttatcacg
     5401 taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta
     5461 aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact
     5521 taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg
     5581 cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc
     5641 aattttacgc agactatctt tctagggtta aaaaagattt gcgctttact cgacctaaac
     5701 tttaaacacg tcatagaatc ttcgtttgac aaaaaccaca ttgtgttggg tcgccctata
     5761 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5821 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     5881 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcaaa
     5941 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     6001 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     6061 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     6121 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     6181 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     6241 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     6301 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     6361 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     6421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7681 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7921 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8281 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8401 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8461 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8521 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8581 aaagggaacc tcccccggta ttcacgacag caggctgaat aataaaaaaa ttagaaacta
     8641 ttatttaacc ctagaaagat aatcatattg tgacgtacgt taaagataat catgcgtaaa
     8701 attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata
     8761 ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac aatttattta
     8821 tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa agtaacaaaa
     8881 cttttaaaca ttctctcttt tacaaaaata a
//
