LOCUS       sequence-274080-        8699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP dre/rox reporter piggyBac transposon (bpA 2x SV40pA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-R-bpA-2xSV40pA-R-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        189..568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        570..847
                     /label=chicken beta-actin promoter
     intron          848..1864
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1788..1810
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1872..1891
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1924..1955
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    1976..2200
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2385..2506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2618..2739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2757..2788
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             join(2797..2799,2800..3510)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2797..3510
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3541..3543,3544..4257)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3541..4254
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    4294..4882
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4347..4367)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4765..4776)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4927..5151
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5505..5539
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5626..5645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5627..5645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5652..5669)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5652..5668)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5661..5683)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5810..6265)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5947..5968)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6159..6178
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6292..6396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6397..6465,6466..7257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6615..6634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7428..8016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7917..7936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8170..8187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8304..8325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8340..8345,8346..8363,8364..8370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8378..8394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8383..8405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8402..8418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8402..8418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8437..8457
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8439..8457
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8521..8583
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ttatttatta aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttaa
       61 acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc atgttgtatt
      121 ataaaataag taattagctt aacctataca taatagaaac aaattatact tattaaccgg
      181 tcatggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      241 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      301 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      361 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      421 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      481 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      541 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
      601 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      661 tgcagcgatg ggggcggggg gggggggggg gcgcgcgccg gggggggggg gggggggggg
      721 gggggggggs ggggsgrggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      781 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      841 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      901 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      961 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     1021 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     1081 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     1141 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1201 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1261 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1321 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1381 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     1441 cggggcgggg ccgcctcggg ccggggrggg ctcgggggag gggcgcggcg gcccccggag
     1501 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1561 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     1621 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     1681 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1741 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1801 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     1861 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct
     1921 agctaacttt aaataattgg cattatttaa agttaagctc gctgatcagc ctcgactgtg
     1981 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     2041 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     2101 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     2161 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     2221 agctggggct cgactagagc ttgcggaacc cttaccaccg cggtggcggc cgctctagaa
     2281 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     2341 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacta gaatcataat
     2521 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2581 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2641 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2701 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat aactaaataa cctaggtaac
     2761 tttaaataat tggcattatt taaagttagg cgcgccatga gcaaaggaga agaacttttc
     2821 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     2881 gtccgtggag agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc
     2941 actactggaa aactacctgt tccgtggcca acacttgtca ctactctgac ctatggtgtt
     3001 caatgctttt cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg
     3061 cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg
     3121 cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaagggtatt
     3181 gattttaaag aagatggaaa cattcttgga cacaaactcg aatacaactt taactcacac
     3241 aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc
     3301 cacaacgttg aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     3361 ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg
     3421 aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg
     3481 attacacatg gcatggatga gctctacaaa ggttctgctg gatctgcagc aggatctggt
     3541 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     3601 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga
     3661 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
     3721 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg
     3781 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
     3841 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
     3901 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa
     3961 ctcgaataca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
     4021 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac
     4081 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     4141 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     4201 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaatgatta
     4261 attaatgatc atacagtgct ctgagtacgc tataatcaac ctctggatta caaaatttgt
     4321 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4381 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4441 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4501 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4561 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4621 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4681 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4741 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4801 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4861 tccctttggg ccgcctcccc gcaggatcca ctagagcggc cgcctagagc tcgctatcag
     4921 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4981 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5041 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5101 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5161 cggaaagaac cagctggggc tcgactagag cttgcggaac ccttaagctt ttttattata
     5221 tattagtcac gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     5281 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     5341 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     5401 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     5461 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     5521 gcagactatc tttctagggt taaaaaagat ttgcgcttta ctcgacctaa actttaaaca
     5581 cgtcatagaa tcttcgtttg acaaaaacca cattgtgttg ggtcgcccta tagtgagtcg
     5641 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5701 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5761 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca aattgtaagc
     5821 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     5881 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     5941 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     6001 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     6061 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     6121 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     6181 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     6241 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6301 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6361 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6421 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6481 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6541 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6601 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6661 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6721 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6781 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6841 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6901 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6961 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7021 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7081 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7141 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7201 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7261 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7321 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7561 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7801 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8041 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8101 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8161 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8221 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8281 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8341 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8401 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     8461 cctcccccgg tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa
     8521 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc
     8581 atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt
     8641 tattatattt acacttacat actaataata aattcaacaa acaatttatt tatgtttat
//
