LOCUS       sequence-274081-        3412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SapTrap mKate2-GLO donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3412)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD376
FEATURES             Location/Qualifiers
     source          1..3412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..87)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     136..158
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     150..167
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     151..167
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    183..282
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1151..1250)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1267..1286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1268..1286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1291..1307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1291..1307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1409..1428)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1420..2229
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1487..1506)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2376..2964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2865..2884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3118..3135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3294..3321)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
       61 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      121 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      181 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      241 aatgcttttt tataatgcca actttgtaca aaaaagcagg ctgctcttcg atggtttccg
      301 agttgatcaa ggagaatatg cacatgaaac tctacatgga aggaaccgtc aacaatcatc
      361 atttcaaatg tacttcagaa ggagaaggaa aaccatacga gggaactcag accatgagaa
      421 tcaaggctgt tgagggaggt aagtttaaaa aatacgtgtt cataacttga tacgaatata
      481 aattttcagg accacttcca ttcgcgttcg acattcttgc cacttcgttc atgtatggaa
      541 gcaagacttt cattaatcac acacaaggga tcccggattt cttcaagcaa tctttcccag
      601 aaggattcac atgggagaga gttaccactt acgaggatgg aggagttctt accgccaccc
      661 aagacacatc cctccaaggt aagtttaaaa gattatagca atgttcgtct aaatataata
      721 aattttcaga tggatgtctg atctacaacg tcaagatcag aggagtcaat ttcccatcaa
      781 atggaccagt tatgcagaag aagactttgg gctgggaggc ttccaccgag accctttacc
      841 cagcagatgg aggtctcgag ggccgcgctg atatggctct caaactcgtc ggaggaggac
      901 acttgatctg caatttaaaa accacctacc ggtcgaaggt aagtttaaaa tcaattttta
      961 atagcttata taataataca aattttcaga agccagccaa gaatctcaaa atgccaggag
     1021 tttactatgt tgaccgtcgt cttgagcgca ttaaagaggc cgataaggaa acgtacgttg
     1081 aacaacatga agttgccgtt gctcgatatt gtgatctccc atccaagctc ggacatcgta
     1141 agcgaagagc acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1201 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1261 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1321 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1381 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1441 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1501 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1561 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1621 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1681 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1741 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1801 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1861 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1921 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1981 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2041 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2101 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2161 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2221 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2281 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2341 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2401 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2461 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2521 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2581 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2641 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2701 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2761 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2821 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2881 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2941 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3001 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3061 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3121 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3181 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3241 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3301 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3361 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc gg
//
