LOCUS       sequence-274082-        8783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF1A 400-1038.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF17 tail 400-1038
FEATURES             Location/Qualifiers
     source          1..8783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..275
                     /label=chicken beta-actin promoter
     primer_bind     1218..1240
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1308..1310,1311..1313,1314..2021)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1353..1374)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1614..1633)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1961..1982
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4202..4336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4239..4258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4293..4312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4367..4383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4367..4383)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4380..4402)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4391..4407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4415..4421,4422..4439,4440..4445))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4460..4481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4598..4615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4769..5357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4849..4868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5528..6319,6320..6388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6151..6170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6389..6493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6561..6579
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6617..6639)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6739..6758
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6952..6974
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6966..6983
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6967..6983
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggc
      121 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      181 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      241 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
      301 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
      361 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
      421 aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc cgggagggcc
      481 ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      541 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
      601 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
      661 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
      721 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
      781 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
      841 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
      901 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
      961 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1021 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     1081 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     1141 ccgtcccctt ctccatctcc agcctcgggg ctgtccgcag ggggacggct gccttcgggg
     1201 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggggttt atatcttccc
     1261 ttctctgttc ctccgcagcc cccaagcttg gcgcgcctag gcctaccatg gtgagcaagg
     1321 gcggggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1381 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1441 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1501 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1561 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1621 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1681 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1741 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1801 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1861 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1921 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1981 tgaccgccgc cgggatcact ctcggcatgg acgagctgta cggcggagga tccggaggcg
     2041 gctctggcgg agggctgtac aagcggaccg gcgctagcgg aggcggatct ggaggcggga
     2101 gcggaggcgg ttccggaggc gggactagtt atccgtacat gcaggacacg gaggctgaga
     2161 agcagttgat ccgagaggag tatgaggagc gcctggcccg cctgaaggct gactacgagg
     2221 cagagcaaga gtctcgggtc cggctgcagg aggacattac tgccatgcgt aactcgtatg
     2281 acgtgaagct gtccacactg caggagaatc tgcgcaagga gaaggagaca gaggccattc
     2341 tcaaggcgga agtcctgtgt aagacggagg tcatgtccag ggccgagtta gccagcgggc
     2401 ccgagtactc acctcctttg cagtatgaga ctgcggtgaa gcccaccatc ctctccatgc
     2461 ccgacatgcc acccagtggc aaggtcacca agagccaagc gcccctcgct ttcgaggaac
     2521 cacacgggga gacctccagg tccgagtttt cctttgagtc caatgagtgt tccactctcg
     2581 aagactctgc cacatcagag gccttccctg ggccagagga gttttccaac atggagttct
     2641 ccatggctgc ggcactgacc gagagcaggt accttcctga ggagtatctc ggcggccagg
     2701 aggctgctgc atctccgctg gaggcagagc gctatgtcca ggagaacgag ccatcgctgg
     2761 agcccctgcg gatactggca agcctgcagg atccttttgc tgaagtggag gccaagctgg
     2821 ccagactgtc ctctactgtg gccatgtcgg actcatccca gaccgtcgtc ccccagatcc
     2881 ctaagcagca gccatcctcg gctgacctgc tggagcccag cgacaccaag tcagaagctg
     2941 atgtggctga caacgtcgtc gtcctcggga ctgagcctga tgtaaacctg agagtggctg
     3001 aggaggtggt ttctgaggca gagactggcg tgtggatgga gtctgaagcc caggtggccc
     3061 atgtggccca ggtgtctgag gaggcccagc ctcagccctt gctggccatg gtgagcgtga
     3121 ggagggaaag tgtgggtgta gaagtggcag tgttgacgga ggaggagctg cagcctgtgg
     3181 atcaacagca ggtgctggcc cgcttgcagt tgctggagca gcaggtggtg gggggcgagc
     3241 aggctaaaaa caaggatctg agggagaagc acaagcggcg gaagcggtac gcagatgagc
     3301 gcaagaagca gctggtggcc gcgctgcaga actcggacga ggatggcggg gactgggtgc
     3361 tgctcaacgt ctatgactct atccaggagg aagtgagggc caagagcaag ctgctggaga
     3421 agatgcagag gaagctccgg gcagcagagg tagaaatcaa ggacctgcag tcagagttcc
     3481 agctggagaa gattgattac ctggcaacca tccgccggca ggagcgggac tccatgctct
     3541 tccagcaact cctggagcag gtgcagcctc tcatccgcag agactgtaac tatagcaacc
     3601 tggagaagat caggcgagag tcgagctggg atgaggataa tggcttctgg aagattccag
     3661 atcccatcat actgaaaacc agcctcccag tagttccaac tgggacacag aacaagccag
     3721 ctcgaaaaac ctctgcagta gacagtgggg agccacacat gcaggaggaa gacaggtata
     3781 agctgatgct cagccgaagc gacagtgaga atatcgctag caactacttc cgctccaagc
     3841 gggccagcca gatcctcagc acggacccca tgaagagcct cacatatcac aactcaccac
     3901 cggggctaaa ttcctctctg agcaacaact ctgccttgcc gcccacccag accccggaaa
     3961 tgccccagcc ccggcccttc cgcctagagt cccttgacat ccccttctcc aaagccaagc
     4021 gtaaaaaaag caaaaacagc ttcggtggtg agcctctgtg atccagctgc ctgctgctat
     4081 ctgcttgaat tcattgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4141 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4201 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4261 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4321 ttatcatgtc tggatccccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc
     4381 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4441 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4501 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4561 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4621 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4681 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4741 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4801 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4861 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4921 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4981 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5041 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5101 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5161 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5221 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5281 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5341 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5401 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5461 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5521 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5581 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5641 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5701 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5761 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5821 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5881 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5941 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6001 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6061 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6121 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6181 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6241 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6301 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6361 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6421 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6481 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     6541 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     6601 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6661 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6721 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     6781 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     6841 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     6901 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     6961 gacgttgtaa aacgacggcc agtgccaagt tgggatcttt gcattggccc acggctctca
     7021 ggatggggat gctccccttc agcacccggt tccccttgga aactgatggt cctggctctg
     7081 tggcatggca gtggcactgt gaggagcccc ctaccagcag cacacagtgg ggttggcact
     7141 gccacgctcc ggatgccgcg ctctgatcca accccataat caagggaacc cgaattgccc
     7201 catcattgcc cccaccaccc ccatcctgcc gggccctcac accccacgct gccttctggt
     7261 gacattcccc agcccaaacc cacggcttca tggctaccgc ggggcatttc ccattgccgc
     7321 cccattatca gctctgcaca cctcccgctg tacccatgcc tcgtggctgc ccttctttga
     7381 cgtataatct tctaattaat acccggcctt gtcaaagtgg agcacaaacg ttaattaatt
     7441 ccccagcagg caggtaatta acagtgtgac tccctttttg ctgcgagtgg ggctgataca
     7501 gagagatgtg gcactatgga gcccacgggg tcctggcact gggtgcccac ggaggtcccc
     7561 atgtgctgca gtgtcaccgc ctccgaggag acagtattgt ccctgcggtg tccctgcagc
     7621 tcagctctgt ccacagggcc acctccagtt tggaggggac acaatgcagc cccgatgcaa
     7681 cccatcctcg cagcatccca gggacaaaga ccccactgca agaccgcaca cagggctggg
     7741 tcccgctccc ctaatatcta cagtgcttct tgcatggccc cttaatcaat gcagttaatc
     7801 agcatgcgct catgcaccgc tctggagctg caaagcccct cgcagcgctg ctcaccaaca
     7861 ccgcgcaccg ccccggccca gcctgcagca cgcgctgcaa acaggaaaga aacaaaatat
     7921 tgcccaaatg taggcaaagg cattcggctg ccttgacctc cgccgggccg ggccctgcct
     7981 gactcagctc cttactcagc gctcgcttcc tccctccggc tgccaccgcc gcagcgcaca
     8041 ccctgacaaa gagtggccct taacgggctc tgaggtgcac ccagcagtgc actcagcagt
     8101 ccaagggccg gcctggaggt ttgcaccgct acgtgctgac attagcattg aacttggccc
     8161 tgggtagtgc tgcaggccgg gcggggtggg tgtagagagt gcagcgcgcg ttgcacccgg
     8221 tgccccttcc cctcccttgc atcccagcag gctgcacccc agcaccaggc ccgtgcatgc
     8281 atgctcctgg tgttattgca gcctggtgca tgcatgcgtc ttgatggtgc agcgctgtgc
     8341 atgcatcctc cttggtgtgt agcagtctag tgcatgcata cccctcggtg ttattgctgc
     8401 tctgtgcacg cacgctcatt gtatcacttc atcccagtgc atgcactcac actggagcga
     8461 ttgctgctcg gtgcacgcac actcattgta tcactgcagc tcagtggctg cacgcacacc
     8521 ggtgttattg ctgctcggtg cgtgcatgca catcagtgtc gctgcagctc agtgcatgca
     8581 cgctcattgc ccatcgctat ccctgcctct cctgctggcg ctccccggga ggtgacttca
     8641 aggggaccgc aggaccacct cgggggtggg gggagggctg cacacgcgga ccccgctccc
     8701 cctccccaac aaagcactgt ggaatcaaaa aggggggagg ggggatggag gggcgcgtca
     8761 cacccccgcc ccacaccctc acc
//
