LOCUS       sequence-274087-        5799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes region -2761 to -1814 of CCR5 promoter HHE Haplotype fused 
            to firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Joshi A, Punke EB, Sedano M, Beauchamp B, Patel R, Hossenlopp C, 
            Alozie OK, Gupta J, Mukherjee D, Garg H
  TITLE     CCR5 promoter activity correlates with HIV disease progression by 
            regulating CCR5 cell surface expression and CD4 T cell apoptosis.
  JOURNAL   Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1038/s41598-017-00192-x.
  PUBMED    28331180
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-22"; volume: "7(1):232. doi"; pages: " 
            10.1038/s41598-017-00192-x"
COMMENT     Sequence Label: pGL3 CCR5 HHE Short
FEATURES             Location/Qualifiers
     source          1..5799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    131..179
                     /note="synthetic polyadenylation signal"
     misc_feature    193..284
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     233..252
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      1354..1363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1360..3012
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1426..1444)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3053..3174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3064..3083)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3118..3137
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3422..3439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3593..4181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3673..3692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4352..5143,5144..5212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4975..4994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5213..5317)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5344..5772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5431..5450)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5641..5662
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg
      121 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
      181 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
      241 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccctt
      301 cagatagatt atatctggag tgaagaatcc tgccacctat gtatctggca tagtgtgagt
      361 cctcataaat gcttactggt ttgaagggca acaaaatagt gaacagagtg aaaatcccca
      421 ctaagatcct gggtccagaa aaagatggga aacctgttta gctcacccgt gagcccatag
      481 ttaaaactct ttagacaaca ggttgtttcc gtttacagag aacaataata ttgggtggtg
      541 agcatctgtg tgggggttgg ggtgggatag gggatacggg gagagtggag aaaaagggga
      601 cacagggtta atgtgaagtc caggatcccc ctctacattt aaagttggtt taagttggct
      661 ttaattaata gcaactctta agataatcag aattttctta accttttagc cttactgttg
      721 aaaagccctg tgatcttgta caaatcattt gcttcttgga tagtaatttc ttttactaaa
      781 atgtgggctt ttgactagat gaatgtaaat gttcttctag ctctgatatc ctttattctt
      841 tatattttct aacagattct gtgtagtggg atgagcagag aacaaaaaca aaataatcca
      901 gtgagaaaag cccgtaaata aaccttcaga ccagagatct attctctagc ttattttaag
      961 ctcaacttaa aaagaagaac tgttctctga ttcttttcgc cttcaataca cttaatgatt
     1021 taactccacc ctccttcaaa agaaacagca tttcctactt ttatactgtc tatatgattg
     1081 atttgcacag ctcatctggc cagaagagct gagacatccg ttcccctaca agaaactctc
     1141 cccggtaagt aacctctcag ctgcttggcc tgttagttag cttctgagat gagtaaaaga
     1201 ctttacagga aacccataga agacatttgg caaacaccaa gtgctcatac aattatctta
     1261 aaatataatc tttaagataa ggaaagggtc acagtttgga atgctcgaga tctgcgatct
     1321 aagtaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
     1381 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
     1441 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
     1501 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
     1561 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     1621 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     1681 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     1741 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
     1801 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
     1861 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
     1921 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
     1981 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
     2041 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     2101 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     2161 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
     2221 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
     2281 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
     2341 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
     2401 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     2461 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
     2521 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
     2581 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     2641 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
     2701 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
     2761 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     2821 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     2881 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     2941 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     3001 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
     3061 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3121 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3181 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     3241 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag
     3301 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     3361 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     3421 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3481 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3541 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3601 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3661 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3721 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3781 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3841 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3901 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3961 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4021 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4081 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4141 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4201 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4261 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4321 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4381 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4441 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4501 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4561 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4621 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4681 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4741 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4801 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4861 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4921 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4981 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5041 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5101 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5161 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5221 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5281 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5341 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5401 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5461 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5521 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5581 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5641 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5701 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5761 ttaacgcgaa ttttaacaaa atattaacgc ttacaattt
//
