LOCUS       sequence-274090-       11444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and
            a repeat-spacer-repeat array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Leenay RT, Vento JM, Shah M, Martino ME, Leulier F, Beisel CL
  TITLE     Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed 
            That Editing Outcomes Can Vary Across Strains and Between Methods.
  JOURNAL   Biotechnol J. 2019 Mar;14(3):e1700583. doi: 10.1002/biot.201700583. 
            Epub 2018 Sep 20.
  PUBMED    30156038
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2019-03"; volume: "14(3):e1700583. doi"; pages: " 
            10.1002/biot.201700583. Epub 2018 Sep 20"
COMMENT     Sequence Label: pCB578
FEATURES             Location/Qualifiers
     source          1..11444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(3017..3095)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             3391..7497
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3391..7494
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     rep_origin      8039..8627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8528..8547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8781..8798
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8885..8904
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8885..8903
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9472..9507
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   9538..9573
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
ORIGIN
        1 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
       61 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
      121 ttttaaaagc ggtatactag atataacgaa acaacgaact gaatagaaac gaaaaaagag
      181 ccatgacaca tttataaaat gtttgacgac attttataaa tgcatagccc gataagattg
      241 ccaaaccaac gcttatcagt tagtcagatg aactcttccc tcgtaagaag ttatttaatt
      301 aactttgttt gaagacggta tataaccgta ctatcattat atagggaaat cagagagttt
      361 tcaagtatct aagctactga atttaagaat tgttaagcaa tcaatcggaa atcgtttgat
      421 tgcttttttt gtattcattt atagaaggtg gagtttgtat gaatcatgat gaatgtaaaa
      481 cttatataaa aaatagttta ttggagataa gaaaattagc aaatatctat acactagaaa
      541 cgtttaagaa agagttagaa aagagaaata tctacttaga aacaaaatca gataagtatt
      601 tttcttcgga gggggaagat tatatatata agttaataga aaataacaaa ataatttatt
      661 cgattagtgg aaaaaaattg acttataaag gaaaaaaatc tttttcaaaa catgcaatat
      721 tgaaacagtt gaatgaaaaa gcaaaccaag ttaattaaac aacctatttt ataggattta
      781 taggaaagga gaacagctga atgaatatcc cttttgttgt agaaactgtg cttcatgacg
      841 gcttgttaaa gtacaaattt aaaaatagta aaattcgctc aatcactacc aagccaggta
      901 aaagcaaagg ggctattttt gcgtatcgct caaaatcaag catgattggc ggtcgtggtg
      961 ttgttctgac ttccgaggaa gcgattcaag aaaatcaaga tacatttaca cattggacac
     1021 ccaacgttta tcgttatgga acgtatgcag acgaaaaccg ttcatacacg aaaggacatt
     1081 ctgaaaacaa tttaagacaa atcaatacct tctttattga ttttgatatt cacacggcaa
     1141 aagaaactat ttcagcaagc gatattttaa caaccgctat tgatttaggt tttatgccta
     1201 ctatgattat caaatctgat aaaggttatc aagcatattt tgttttagaa acgccagtct
     1261 atgtgacttc aaaatcagaa tttaaatctg tcaaagcagc caaaataatt tcgcaaaata
     1321 tccgagaata ttttggaaag tctttgccag ttgatctaac gtgtaatcat tttggtattg
     1381 ctcgcatacc aagaacggac aatgtagaat tttttgatcc taattaccgt tattctttca
     1441 aagaatggca agattggtct ttcaaacaaa cagataataa gggctttact cgttcaagtc
     1501 taacggtttt aagcggtaca gaaggcaaaa aacaagtaga tgaaccctgg tttaatctct
     1561 tattgcacga aacgaaattt tcaggagaaa agggtttaat agggcgtaat aacgtcatgt
     1621 ttaccctctc tttagcctac tttagttcag gctattcaat cgaaacgtgc gaatataata
     1681 tgtttgagtt taataatcga ttagatcaac ccttagaaga aaaagaagta atcaaaattg
     1741 ttagaagtgc ctattcagaa aactatcaag gggctaatag ggaatacatt accattcttt
     1801 gcaaagcttg ggtatcaagt gatttaacca gtaaagattt atttgtccgt caagggtggt
     1861 ttaaattcaa gaaaaaaaga agcgaacgtc aacgtgttca tttgtcagaa tggaaagaag
     1921 atttaatggc ttatattagc gaaaaaagcg atgtatacaa gccttattta gtgacgacca
     1981 aaaaagagat tagagaagtg ctaggcattc ctgaacggac attagataaa ttgctgaagg
     2041 tactgaaggc gaatcaggaa attttcttta agattaaacc aggaagaaat ggtggcattc
     2101 aacttgctag tgttaaatca ttgttgctat cgatcattaa agtaaaaaaa gaagaaaaag
     2161 aaagctatat aaaggcgctg acaaattctt ttgacttaga gcatacattc attcaagaga
     2221 ctttaaacaa gctagcagaa cgccctaaaa cggacacaca actcgatttg tttagctatg
     2281 atacaggctg aaaataaaac ccgcactatg ccattacatt tatatctatg atacgtgttt
     2341 gttttttctt tgctgtttag cgaatgatta gcagaaatat acagagtaag attttaatta
     2401 attattaggg ggagaaggag agagtagccc gaaaactttt agttggcttg gactgaacga
     2461 agtgagggaa aggctactaa aacgtcgagg ggcagtgaga gcgaagcgaa cacttgattt
     2521 tttaattttc tatcttttat aggtcattag agtatactta tttgtcctat aaactattta
     2581 gcagcataat agatttattg aataggtcat ttaagttgag catattagag gaggaaaatc
     2641 ttggagaaat atttgaagaa cccgattaca tggattggat tagttcttgt ggttacgtgg
     2701 tttttaacta aaagtagtga atttttgatt tttggtgtgt gtgtcttgtt gttagtattt
     2761 gctagtcaaa gtgattaaat agaattctca tgtttgacag cttatcatcg gagctctgat
     2821 gataagcggt ccaaacatga gaattcctta cgaaatcatc ctgtggagct tagtaggttt
     2881 agcaagatgg cagcgcctaa atgtagaatg ataaaaggat taagagatta atttccctaa
     2941 aaatgataaa acaagcgttt tgaaagcgct tgtttttttg gtttgcagtc agagtagaat
     3001 agaagtatca aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
     3061 ttattttaac ttgctatgct gttttgaatg gttccaacaa gattatttta taacttttat
     3121 aacaaataat caaggagaaa ttcaaagaaa tttatcagcc ataaaacaat acttaatact
     3181 atagaatgat aacaaaataa actacttttt aaaagaattt tgtgttataa tctatttatt
     3241 attaagtatt gggtaatatt ttttgaagag atattttgaa aaagaaaaat taaagcatat
     3301 taaactaatt tcggaggtca ttaaaactat tattgaaatc atcaaactca ttatggattt
     3361 aatttaaact ttttatttta ggaggcaaaa atggataaga aatactcaat aggcttagat
     3421 atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa
     3481 aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct
     3541 cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga
     3601 aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg
     3661 gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac
     3721 aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag
     3781 aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat
     3841 ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt
     3901 gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa
     3961 acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg
     4021 attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc
     4081 ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct
     4141 aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact
     4201 tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt
     4261 ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact
     4321 gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa
     4381 gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc
     4441 ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa
     4501 gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg
     4561 gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt
     4621 ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat
     4681 ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat
     4741 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa
     4801 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca
     4861 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa
     4921 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt
     4981 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat
     5041 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa
     5101 aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca
     5161 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa
     5221 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag
     5281 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
     5341 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
     5401 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
     5461 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa
     5521 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
     5581 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
     5641 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
     5701 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
     5761 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
     5821 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
     5881 aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat
     5941 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
     6001 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
     6061 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
     6121 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
     6181 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
     6241 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc
     6301 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
     6361 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
     6421 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
     6481 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
     6541 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
     6601 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
     6661 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt
     6721 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
     6781 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
     6841 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa
     6901 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
     6961 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
     7021 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
     7081 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
     7141 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
     7201 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
     7261 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt
     7321 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca
     7381 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa
     7441 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgactgaaaa
     7501 aggtggggca gaagttatcc taaaaataaa aaagtaattt agtaatctct aaggattact
     7561 ttttttgttt cggcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7621 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7681 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7741 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7801 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7861 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7921 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7981 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8041 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8101 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8161 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8221 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8281 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8341 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8401 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     8461 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8521 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8581 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8641 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8701 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8761 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8821 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     8881 aaattaatac gactcactat agggagaccg gcctcgagaa gctcgcgtta tcggtccttt
     8941 aattggtgga caaatttaca ttagtctcgg acattctgct cccgccctta tgggatttat
     9001 cttccttatt ctcgctttga ttgttctatc gaaagcgaaa tcaaacgaat aatttataaa
     9061 taaaaaaacc acctctaaaa ggtgattttt atttataaat tacaattttt cagcaatatc
     9121 agtaattgct ttatcaactg ctgctttttg gctatcaatc aaagcaacac gtgctgtaat
     9181 ttgtttaatt gccatttcaa ttgaacgttt caagccttgg ttttctaatt ttggttcaaa
     9241 gaaagcttga gctctcgagt caggggtacc gatcaggaaa ttaaaattgg tctcatataa
     9301 ctgaattatt ttcggaaaat aaagggaatc tgtttacaaa cattaccagt atcgatataa
     9361 tacttaagga ttcttcgaaa attgactact ttgtcttttc cagaagatgt agtataataa
     9421 cacttagaaa tgcattgatg cgaaattgat gtaattcttg aaaacatgta cgttttagag
     9481 ctatgctgtt ttgaatggtc ccaaaacttc gatcgacgaa cgtgtcacta aagccttgtt
     9541 ttagagctat gctgttttga atggtcccaa aacatgcggc cgcgccacgc cgcaaacccc
     9601 gcccctgaca gggcggggtt tcgccgcgat caactcattg cagacatttg cagtacccat
     9661 ggtgagtgcc tccttataat ttattttgta gttccttcga acgaaatcat tgtatctaac
     9721 aaacttcaga atttaatcag agccgtttat tatgctcgcg ttatcgacaa taatattatt
     9781 accaatactt tctcaagata gaattaagac tgttttagat ttgttaatgt ttctattgtc
     9841 agtatagtta taagactaga tctgatccgt agcggttttc aaaatttgca accaggaatg
     9901 aattactatc ccttttatca agaagcgcaa aagaaaaacg aaatgataca ccaatcagtg
     9961 caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg caccatatca
    10021 taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa ataagactta
    10081 gaagcaaact taagagtgtg ttgatagtgc agtatcttaa aattttgtat aataggaatt
    10141 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gtgattacat gaacaaaaat
    10201 ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa
    10261 ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg
    10321 acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc
    10381 aacttatcgt cagaaaaatt aaaactgaat actcgtgtca ctttaattca ccaagatatt
    10441 ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat
    10501 ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg
    10561 attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc
    10621 ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct
    10681 aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat
    10741 aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa
    10801 ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt
    10861 accgttactt atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg
    10921 aaataattct atgagtcgct tttgtaaatt tggaaagtta cacgttacta aagggaatgt
    10981 agataaatta ttaggtatac tactgacagc ttccaaggag ctaaagaggt ccctagcgct
    11041 tagaatcgct ttaggaaaca cgatccagtc caataatcgt cgataaaaac ttttgaaaaa
    11101 ggttggtgaa attacctact tttggaataa tcacaaatca caagtgatta atcacaaatc
    11161 acaagtgatt aatcacttgt ttattaagat attaaaagct ataatttaaa taaagcgtga
    11221 attttattac acaaaaagag gggggagaaa cttggaacta gcatttagag aaagcttaaa
    11281 aaagatgaga ggtaccaaat caaaagaaaa attctcccaa gaattagaaa tgagtagatc
    11341 aaattattca cgaatagaat caggaaaatc agatccaacc ataaaaacac tagaacaaat
    11401 tgcaaagtta actaactcaa cgctagtagt ggatttaatc ccaa
//
