LOCUS       sequence-274092-         298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and
            a repeat-spacer-repeat array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Leenay RT, Vento JM, Shah M, Martino ME, Leulier F, Beisel CL
  TITLE     Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed 
            That Editing Outcomes Can Vary Across Strains and Between Methods.
  JOURNAL   Biotechnol J. 2019 Mar;14(3):e1700583. doi: 10.1002/biot.201700583. 
            Epub 2018 Sep 20.
  PUBMED    30156038
REFERENCE   2  (bases 1 to 298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2019-03"; volume: "14(3):e1700583. doi"; pages: " 
            10.1002/biot.201700583. Epub 2018 Sep 20"
COMMENT     Sequence Label: pCB578
FEATURES             Location/Qualifiers
     source          1..298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   197..232
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   263..298
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
ORIGIN
        1 ggaaattaaa attggtctca tataactgaa ttattttcgg aaaataaagg gaatctgttt
       61 acaaacatta ccagtatcga tataatactt aaggattctt cgaaaattga ctactttgtc
      121 ttttccagaa gatgtagtat aataacactt agaaatgcat tgatgcgaaa ttgatgtaat
      181 tcttgaaaac atgtacgttt tagagctatg ctgttttgaa tggtcccaaa acttcgatcg
      241 acgaacgtgt cactaaagcc ttgttttaga gctatgctgt tttgaatggt cccaaaac
//
