LOCUS       sequence-274101-        9393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of GFP with a nuclear localization sequence, under 
            the human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Miller JD, Ganat YM, Kishinevsky S, Bowman RL, Liu B, Tu EY, Mandal 
            PK, Vera E, Shim JW, Kriks S, Taldone T, Fusaki N, Tomishima MJ, 
            Krainc D, Milner TA, Rossi DJ, Studer L
  TITLE     Human iPSC-based modeling of late-onset disease via progerin-induced
            aging.
  JOURNAL   Cell Stem Cell. 2013 Dec 5;13(6):691-705. doi: 
            10.1016/j.stem.2013.11.006.
  PUBMED    24315443
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11"; journalName: "Cell Stem Cell"; date: 
            "2013-12-5- 5"; volume: "13"; issue: "6"; pages: "691-705"
COMMENT     Sequence Label: pLenti-hSyn-nucGFP
FEATURES             Location/Qualifiers
     source          1..9393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    208..229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(244..249,250..267,268..274)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    282..298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     287..309
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     306..322
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     306..322
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    371..492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(382..401)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     436..455
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(622..996)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1015..1062)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1110..1439)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1124..1259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1178..1197)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1424..1444
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1453..1881)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1563..1584)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1775..1794
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1927..2151
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2140..2157
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2183..2363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     2868..2884
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2869..2885
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2888..3476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2994..3005
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3403..3423
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3522..3542)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3546..3566)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3570..3590)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3615..4331)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        complement(4345..4792)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    4867..4984
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5282..5326)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5511..5744
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6237..6362
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6409..6589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6607..6805)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6632..6652)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6807..7186
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7358..7377
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7452..7556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7557..7625,7626..8417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7775..7794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8588..9176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9077..9096
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
       61 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      121 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
      181 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      241 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      301 tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtat
      361 acagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      421 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      481 aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
      541 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
      601 atcgaaatct cgtagcacgt gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc
      661 ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct cggtcatggc
      721 cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg cgtacagctc
      781 gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct ggtcctggac
      841 cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa
      901 gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc
      961 ggtgagcacc ggaacggcac tggtcaactt ggccatggtt tagttcctca ccttgtcgta
     1021 ttatactatg ccgatatact atgccgatga ttaattgtca acacgtgctg atcagatccg
     1081 aaaatggata tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct
     1141 cctcactact tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa
     1201 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     1261 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     1321 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     1381 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     1441 ttccacagaa ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     1501 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     1561 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     1621 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     1681 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     1741 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     1801 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     1861 atgcgccgct acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct
     1921 cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
     1981 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
     2041 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
     2101 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
     2161 atcagcgggt ttaaacgggc cctgctagag attttccaca ctgactaaaa gggtctgagg
     2221 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     2281 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     2341 atctggtcta accagagaga cccagtacag tccggatgca gctctcgggc catgtgatga
     2401 aatgctaggc ggctgtcaaa cctccactct aatacttctc tctccgggtc atccatccca
     2461 tgcacgctca cagggtgtaa caagcgggtg ttctctcctt cattggcttc ttctaccttc
     2521 tcttgctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgatccct
     2581 ggccctggtg tgtagttctg ccaatcaggg aagtagcctt gtgtgtggta gatccacaga
     2641 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt
     2701 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     2761 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg
     2821 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg aggtcgacgg
     2881 tatcgatgcg gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga
     2941 cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg
     3001 gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc
     3061 agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc
     3121 cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg
     3181 agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg
     3241 ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac
     3301 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg
     3361 gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca
     3421 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg
     3481 ataagcttga tatcgaattc ttatctagat ccggtggatc ctacctttct cttctttttt
     3541 ggatctacct ttctcttctt ttttggatct acctttctct tcttttttgg atcagctcga
     3601 gatctgagtc cggacttgta cagctcatcc atgccgtggg tgatggcggc ggcggtcacg
     3661 aagccgaagt agatcatgtg atcgcgcttc tcgttggggt ccttggacag ggcgctctgg
     3721 gtggacaggt agtggttatc gggcagcagc acagggccat cgccgatggg ggtattctgc
     3781 tggtagtggt cggccagctg cacgctgcca tcctcgatgt tgtggcggat cttgaagttc
     3841 accttgatgc cattcttggc cttgtcggtc atgatgtaca cattgtgggc gttgtagttg
     3901 tactccatct tattgcccag gatgttgcca tcctccttga aatcggtgcc ggtcagctcg
     3961 atgcgattca ccagggtatc gccctcgaac ttcacctcgg cgcgcgactt gtagttgccg
     4021 tcatcctcga agaagatggt gcgctcctgg atgtagccct caggcatggc gctcttgaag
     4081 aagtcgtgct gcttcatgtg atcggggtag cgtgagaagc actgcacgcc gtagctcagg
     4141 gtggtcacca gggtgggcca gggcacaggc agcttgccgg tggtgcagat gaacttcagg
     4201 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgc tcacgctgaa cttgtggcca
     4261 ttcacatcgc cattcagctc gatcaggatg ggcacgatgc cggtgaacag ctcggcgccc
     4321 ttgctcacca taccggtttc tcgactgcgc tctcaggcac gacacgactc ctccgctgcc
     4381 caccgcagac tgaggcagcg ctgagtcgcc ggcgccgcag cgcagatggt cgcgcccgtg
     4441 cccccctatc tcgcgcctcg cgtggtgcgg tccggctggg ccggcggcgg cgcggacgcg
     4501 accaaggtgg ccgggaaggg gagtttgcgg gggaccggcg agtgacgtca gcgcgccttc
     4561 agtgctgagg cggcggtggc gcgcgccgcc aggcgggggc gaaggcactg tccgcggtgc
     4621 tgaagctggc agtgcgcacg cgcctcgccg catcctgttt cccctccccc tctctgatag
     4681 gggatgcgca atttggggaa tgggggttgg gtgcttgtcc agtgggtcgg ggtcggtcgt
     4741 caggtaggca cccccacccc gcctcatcct ggtcctaaaa cccacttgca ctcatacgca
     4801 gggccctctg cagtctagac acttaattaa ccaaactgga tctctgctgt ccctgtaata
     4861 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
     4921 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     4981 aaaattgtgg atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt
     5041 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     5101 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     5161 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     5221 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     5281 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     5341 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     5401 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     5461 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     5521 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     5581 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     5641 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     5701 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     5761 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     5821 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     5881 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
     5941 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     6001 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     6061 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
     6121 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     6181 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
     6241 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
     6301 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
     6361 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
     6421 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     6481 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     6541 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa
     6601 aacgcgctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
     6661 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     6721 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     6781 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     6841 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     6901 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     6961 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     7021 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     7081 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     7141 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg
     7201 gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg
     7261 tcggtcaagc cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc
     7321 aacacacaag cagggagcag atactggctt aactatgcgg catcagagca gattgtactg
     7381 agagtgcacc ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc
     7441 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7501 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7561 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7621 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7681 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7741 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7801 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7861 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7921 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7981 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8041 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8101 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8161 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8221 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8281 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8341 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8401 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8461 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8521 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8581 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8641 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8701 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8761 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8821 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8881 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8941 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9001 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9061 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9121 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9181 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9241 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9301 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9361 gcccaatacg caaaccgcct ctccccgcgc gtt
//
