LOCUS       sequence-274184-        6939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses proteins in C. elegans cholinergic neurons in head, 
            ventral nerve cord, and tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Williams SL, Lutz S, Charlie NK, Vettel C, Ailion M, Coco C, Tesmer 
            JJ, Jorgensen EM, Wieland T, Miller KG
  TITLE     Trio's Rho-specific GEF domain is the missing Galpha q effector in 
            C. elegans.
  JOURNAL   Genes Dev. 2007 Nov 1;21(21):2731-46. doi: 10.1101/gad.1592007. Epub
            2007 Oct 17.
  PUBMED    17942708
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.1592007"; journalName: "Genes Dev"; date: "2007-11-1- 
            1"; volume: "21"; issue: "21"; pages: "2731-46"
COMMENT     Sequence Label: KG#493
FEATURES             Location/Qualifiers
     source          1..6939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1553..1575)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1675..1694
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1772..1790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1858..1962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1963..2031,2032..2823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2181..2200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2994..3582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3483..3502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3736..3753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3870..3891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3906..3911,3912..3929,3930..3936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3944..3960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3949..3971
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3968..3984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3968..3984
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      4131..4140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccccccctc tgagccaatc aatcatgtgc attttgaatt ggcgccaagg caatcttcgt
       61 aaaaaataac aatagagtca tccctcagaa cagaaaagaa acccgcccgg gggctcaaat
      121 acacatgtca tttttctttt ttcgcctgat ttttagccta aattagtgtt tagcaatttt
      181 ttaaggtaac tcggtggagt catttgaact ttttaaacta aaatttcaga aaaaattctt
      241 tttttttgtc aaaaaataat tttcccgccg gaatttttca aaaaaataat ttttatttta
      301 tttttcaagt tccttgtttc ctctttattt ttttattttg cccccaaaag ttttacacta
      361 aaatatttaa ttttcaggta attcatcgaa agtctttcta ttttccgcat ctcttgttca
      421 agggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat
      481 tttcaggagg acccttggct agcagctctc ctgcagggtt cgacggtacc ggtcagatat
      541 cactcgagga gctcccgaga tcttcagcgc tcagcattcg tagaattgta agtttaaact
      601 ttctcatact aactaacgcg ctctatttaa attttcagaa ttccaactga gcgccggtcg
      661 ctaccattac caacttgtct ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt
      721 ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg
      781 cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct
      841 cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt
      901 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
      961 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
     1021 acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat
     1081 catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca
     1141 atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa
     1201 agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct
     1261 gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct
     1321 tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt
     1381 tgaggctcag tggaaggtga gtagaagttg ataatttgaa agtggagtag tgtctatggg
     1441 gtttttgcct taaatgacag aatacattcc caatatacca aacataactg tttcctacta
     1501 gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1561 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1621 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     1681 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1741 ggagaaaata ccgcatcagg cggccttaag ggcctcgtga tacgcctatt tttataggtt
     1801 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1861 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1921 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1981 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2041 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2101 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2161 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2221 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2281 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2341 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2401 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2461 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2521 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2581 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2641 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2701 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2761 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2821 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2881 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2941 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3001 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3061 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3121 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3181 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3241 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3301 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3361 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3421 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3601 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3661 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3721 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3781 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3841 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3901 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3961 tttcacacag gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct
     4021 tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca
     4081 acgatggata cgctaacaac ttggaaatga aataagcttg catgctcccg gccgccatgg
     4141 ccgcgggatt acaccaatca tttctcccct ccgaaatgtg tagtcgtcat gacaaagtgg
     4201 tgacactgga ttacggtagg ctccgcccac ttcttggaaa agtgacgtgg gctcggtgag
     4261 ccctaaagct tttggttttc acaattttct ggtttttggg atttttgcgg gaattttgag
     4321 aggagaaagt tgggagagaa gatattggaa tggtccccgg ggagctgtac atttatacat
     4381 ctagactgaa gctggggtat tgcaaaaaga gaaaagccta tcactttctt tccatcctgc
     4441 atacttgcca aaaagttttg gctgagctta tttttaaaat tggaattttt tggaattttc
     4501 agaaaagaaa aagttttttc ttttttttta atttaaaaaa tttttgctat ttattgcatg
     4561 gtactacaga aaaacttgaa tgaaataaaa aatttggaaa attcaagaaa actttttttt
     4621 tcatagttta tttattcgag ttttaatgtg gcttttattt tgttcggttt ttgttttaaa
     4681 aagattttat acaaaatttc aagaatttac aatttttccg ttgtaaaaaa tccaaaaaaa
     4741 aatccaaaaa aatagttcag accttttttt aaattttaaa tttaaaaatt caataccaaa
     4801 aaattaaaaa aaattagttt ttccgagaat aaaggaaaaa tgttgaaaaa atattatata
     4861 taaaatttcc agaaaatgtg aaaactccaa aaaactgcag acttttcccc aaactagcta
     4921 ttttttgaat tcattacctt taaaggtgga gtaccgaaat ttaagacttt gcttttttag
     4981 acccaaaatg gtccaaaact accaaatttc gtaatgagac gttctgaaaa tttttcaaaa
     5041 aaatgttatg ggagctcaaa gtttttgaaa aaatggtata tttttagcta attttaacca
     5101 cttcttggcg tcgcagcggt tggaactcaa ttttaattaa attatcatcc ttcaatgtcc
     5161 ttcaatcttt gcaatttcaa tcgaccatac gcgttcagag catttttttt tttcgttttt
     5221 cctcccgtcc agcacatatt taaatattct ctctccccct cccctggttc ctaaaaactt
     5281 gggaaagggg aatttcgaag gaggtggaag cttcttcttc tccccgagca cacacacaca
     5341 tcctctcttc aaaatcttta agaaatagtg cgtgtgaatg actagacgct gaagctaact
     5401 tggtttccgc gaacaatccc aatcagtgcc tgtgccgagg cttcggagaa gaagagattg
     5461 acgtcgtctt tttctttttt tgtgaaacag aataaaaaag aatacgaaat ggactttttc
     5521 acacattttc gttacttttc cagaaaaaaa cgaagctcta gccatggaag tttgaattga
     5581 aaacctaaac cccgatgtgt attcatttga gcttaagcct aagcctaatt ctacgcctaa
     5641 gcataagcct aagcctatgt tcaagcctaa gccaaattgt agaacgttac caaaagatca
     5701 aacctatttt tcctacagaa aaggtctaat gataactgtt aagcgagaga caaggagaga
     5761 tatatatcaa aaactagccc aaactgtgat taacatctat taactgacct caaaaaaaaa
     5821 aatagtagag attaaatact gctcaaaaag tttcttggaa aagtaaattg ttaggtttct
     5881 ggggcctgta aaactggacc gagttttgaa gaattttttt aacttttcaa agtttggcgt
     5941 cactgaaagc tttatattgt acatgttcca ccaattttct tttgtatccg ttttttctgt
     6001 ctcaaaacgg agctcgagcc catgacgtgt ggataccgtg agaatccgcc caattccttc
     6061 acatcgatct catatttctt tcccagttta tattgaattc ccgttgaatt gcggctttgc
     6121 aggggagaag atgtgcgtct ttatattttt ttttctatcc ttacccgccg tgaatccggc
     6181 tcgaattgag ccaattttta tgaatagtag agctaattga cttcgtctcc ctagaattga
     6241 tgaggtaaag ctcgtgggga gatatggaga tttttcgatt ttttttccag taattttcag
     6301 ttcaaaattt gagcttttga gcaggagttt tgagatttgt gccaaaattt cctattattt
     6361 tttcaaatat ttttgctttt gtaaaaaagg tgtagtagaa gttttgtagt gccactattt
     6421 tttttttgaa aattatgttt tggcggaaag ttctttaaaa aagttaatca aaatttttgg
     6481 cgctttagtc aaattaattt tctggcaaaa atattagtaa ttttggcgtg agttcaattt
     6541 tcccattctt aaaatttcac acaattaaga attttaagat ttgggttttt ttttcaacaa
     6601 aaaatcaaat atttacatga aaattcggcg ggaaataaaa aaaaatattc cgattcaatg
     6661 tgtgaataac tttttgcggg gaattcaaat ttgcattatt taaacttttt ttaagctcga
     6721 aaaattgaaa ttcttaaata aataaataaa attttcctcg aattttgaat gtttcgattt
     6781 tttagcgtta ttttttcaaa atttccacta aaactctagt tctaaagttc taaaacttta
     6841 aaaacttcct aatgttgtgg tcttgtgttt ttttggcagc gatttccaaa tttctgaaat
     6901 tccaacaaaa aaaccactac gtcagtttgc atatccaac
//
