LOCUS       sequence-274194-       11497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   St Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati 
            F, Munagala U, Conticello SG, Harris RS
  TITLE     A fluorescent reporter for quantification and enrichment of DNA 
            editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
  JOURNAL   Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 
            10.1093/nar/gky332.
  PUBMED    29746667
REFERENCE   2  (bases 1 to 11497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332."
COMMENT     Sequence Label: plenti-CMV-mCherry-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..11497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             103..147
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             441..467
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     enhancer        784..1087
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1088..1291
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1242..1262
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1288..1312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1328..2035
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1473..1491)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1741..1760
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2060..2113
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2114..2116,2117..2119,2120..2830)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2159..2180)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2420..2439)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2767..2788
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2905..3538
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5178..5195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5349..5937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5429..5448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6108..6899,6900..6968))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6731..6750
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6969..7073)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7141..7159
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7197..7219)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7319..7338
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             9031..9288
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10222..10339
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11182..11415
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cagatttgga ataacatgac ctggatggag tgggacagag aaattaacaa ttacacaagc
       61 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
      121 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
      181 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
      241 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
      301 ctcccaatcc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
      361 gacagagaca gatccattcg attagtgaac ggatccttag cacttatctg ggacgatctg
      421 cggagcctgt gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg
      481 attgtggaac ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta
      541 caatattgga gtcaggagct aaagaatagt gctgttagct tgctcaatgc cacagccata
      601 gcagtagctg aggggacaga tagggttata gaagtagtac aaggagcttg tagagctatt
      661 cgccacatac ctagaagaat aagacagggc ttggaaagga ttttgctata agatgggtgg
      721 cgcggccgct taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      781 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      841 attgacgtca ataatgacgt atgttcccat agtaacgcca attgggactt tccattgacg
      901 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      961 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1021 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1081 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     1141 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1201 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1261 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tccagctggc
     1321 taccggtatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     1381 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     1441 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     1501 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     1561 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     1621 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     1681 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     1741 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     1801 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     1861 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     1921 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     1981 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagcccgg
     2041 tgcagatctc gagtacaagg agggcagagg aagtcttcta acatgcggtg acgtggagga
     2101 gaatcccggc cctatggtca gtaaaggtga agaactgttc accggggtgg tgcccatcct
     2161 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2221 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2281 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     2341 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2401 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2461 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2521 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2581 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2641 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2701 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     2761 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gtatggatga
     2821 attatataaa taaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2881 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga agacaagata
     2941 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     3001 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     3061 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     3121 gcctgcatgg aatggatgac cctgagagag aagtgttaga gtggaggttt gacagccgcc
     3181 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgacatc
     3241 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     3301 ctggggagtg gcgagccctc agatgctgca tataagcagc tgctttttgc ctgtactggg
     3361 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3421 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3481 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcacc
     3541 caggaggtag aggttgcagt gagccaagat cgcgccactg cattccagcc tgggcaagaa
     3601 aacaagactg tctaaaataa taataataag ttaagggtat taaatatatt tatacatgga
     3661 ggtcataaaa atatatatat ttgggctggg cgcagtggct cacacctgcg cccggccctt
     3721 tgggaggccg aggcaggtgg atcacctgag tttgggagtt ccagaccagc ctgaccaaca
     3781 tggagaaacc ccttctctgt gtatttttag tagattttat tttatgtgta ttttattcac
     3841 aggtatttct ggaaaactga aactgttttt cctctactct gataccacaa gaatcatcag
     3901 cacagaggaa gacttctgtg atcaaatgtg gtgggagagg gaggttttca ccagcacatg
     3961 agcagtcagt tctgccgcag actcggcggg tgtccttcgg ttcagttcca acaccgcctg
     4021 cctggagaga ggtcagacca cagggtgagg gctcagtccc caagacataa acacccaaga
     4081 cataaacacc caacaggtcc accccgcctg ctgcccaggc agagccgatt caccaagacg
     4141 ggaattagga tagagaaaga gtaagtcaca cagagccggc tgtgcgggag aacggagttc
     4201 tattatgact caaatcagtc tccccaagca ttcggggatc agagttttta aggataactt
     4261 agtgtgtagg gggccagtga gttggagatg aaagcgtagg gagtcgaagg tgtccttttg
     4321 cgccgagtca gttcctgggt gggggccaca agatcggatg agccagttta tcaatccggg
     4381 ggtgccagct gatccatgga gtgcagggtc tgcaaaatat ctcaagcact gattgatctt
     4441 aggttttaca atagtgatgt taccccagga acaatttggg gaaggtcaga atcttgtagc
     4501 ctgtagctgc atgactccta aaccataatt tcttttttgt tttttttttt ttatttttga
     4561 gacagggtct cactctgtca cctaggctgg agtgcagtgg tgcaatcaca gctcactgca
     4621 gcctcaacgt cgtaagctca agcgatcctc ccacctcagc ctgcctggta gctgagacta
     4681 caagcgacgc cccagttaat ttttgtattt ttggtagagg cagcgttttg ccgtgtggcc
     4741 ctggctggtc tcgaactcct gggctcaagt gatccagcct cagcctccca aagtgctggg
     4801 acaaccgggg ccagtcactg cacctggccc taaaccataa tttctaatct tttggctaat
     4861 ttgttagtcc tacaaaggca gtctagtccc caggcaaaaa gggggtttgt ttcgggaaag
     4921 ggctgttact gtctttgttt caaactataa actaagttcc tcctaaactt agttcggcct
     4981 acacccagga atgaacaagg agagcttgga ggttagaagc acgatggaat tggttaggtc
     5041 agatctcttt cactgtctga gttataattt tgcaatggtg gttcaaagac tgcccgcttc
     5101 tgacaccagt cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5161 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5221 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5281 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5341 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5401 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5461 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5521 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5581 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5641 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5701 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5761 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5821 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5881 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5941 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6001 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6061 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6121 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6181 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6241 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6301 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6361 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6421 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6481 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6541 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6601 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6661 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6721 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6781 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6841 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6901 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6961 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7021 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7081 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     7141 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7201 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7261 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     7321 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     7381 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     7441 gggcgatcgg tgcgggcctc ttcgctatta cgccagggga ggcagagatt gcagtaagct
     7501 gagatcgcag cactgcactc cagcctgggc gacagagtaa gactctgtct caaaaataaa
     7561 ataaataaat caatcagata ttccaatctt ttcctttatt tatttattta ttttctattt
     7621 tggaaacaca gtccttcctt attccagaat tacacatata ttctattttt ctttatatgc
     7681 tccagttttt tttagacctt cacctgaaat gtgtgtatac aaaatctagg ccagtccagc
     7741 agagcctaaa ggtaaaaaat aaaataataa aaaataaata aaatctagct cactccttca
     7801 catcaaaatg gagatacagc tgttagcatt aaataccaaa taacccatct tgtcctcaat
     7861 aattttaagc gcctctctcc accacatcta actcctgtca aaggcatgtg ccccttccgg
     7921 gcgctctgct gtgctgccaa ccaactggca tgtggactct gcagggtccc taactgccaa
     7981 gccccacagt gtgccctgag gctgcccctt ccttctagcg gctgccccca ctcggctttg
     8041 ctttccctag tttcagttac ttgcgttcag ccaaggtctg aaactaggtg cgcacagagc
     8101 ggtaagactg cgagagaaag agaccagctt tacagggggt ttatcacagt gcaccctgac
     8161 agtcgtcagc ctcacagggg gtttatcaca ttgcaccctg acagtcgtca gcctcacagg
     8221 gggtttatca cagtgcaccc ttacaatcat tccatttgat tcacaatttt tttagtctct
     8281 actgtgccta acttgtaagt taaatttgat cagaggtgtg ttcccagagg ggaaaacagt
     8341 atatacaggg ttcagtacta tcgcatttca ggcctccacc tgggtcttgg aatgtgtccc
     8401 ccgaggggtg atgactacct cagttggatc tccacaggtc acagtgacac aagataacca
     8461 agacacctcc caaggctacc acaatgggcc gccctccacg tgcacatggc cggaggaact
     8521 gccatgtcgg aggtgcaagc acacctgcgc atcagagtcc ttggtgtgga gggagggacc
     8581 agcgcagctt ccagccatcc acctgatgaa cagaacctag ggaaagcccc agttctactt
     8641 acaccaggaa aggctggaag ggctaatttg gtcccaaaaa agacaagaga tccttgatct
     8701 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     8761 gatcagatat ccactgacct ttggatggtg cttcaagtta gtaccagttg aaccagagca
     8821 agtagaagag gccaatgaag gagagaacaa cagcttgtta caccctatga gccagcatgg
     8881 gatggaggac ccggagggag aagtattagt gtggaagttt gacagcctcc tagcatttcg
     8941 tcacatggcc cgagagctgc atccggagta ctacaaagac tgctgacatc gagctttcta
     9001 caagggactt tccgctgggg actttccagg gaggtgtggc ctgggcggga ctggggagtg
     9061 gcgagccctc agatgctaca tataagcagc tgctttttgc ctgtactggg tctctctggt
     9121 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9181 aataaagctt gccttgagtg ctcaaagtag tgtgtgcccg tctgttgtgt gactctggta
     9241 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     9301 cagggacttg aaagcgaaag taaagccaga ggagatctct cgacgcagga ctcggcttgc
     9361 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     9421 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc ggtattaagc gggggagaat
     9481 tagataaatg ggaaaaaatt cggttaaggc cagggggaaa gaaacaatat aaactaaaac
     9541 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc cttttagaga
     9601 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     9661 aagaacttag atcattatat aatacaatag cagtcctcta ttgtgtgcat caaaggatag
     9721 atgtaaaaga caccaaggaa gccttagata agatagagga agagcaaaac aaaagtaaga
     9781 aaaaggcaca gcaagcagca gctgacacag gaaacaacag ccaggtcagc caaaattacc
     9841 ctatagtgca gaacctccag gggcaaatgg tacatcaggc catatcacct agaactttaa
     9901 atgcatgggt aaaagtagta gaagagaagg ctttcagccc agaagtaata cccatgtttt
     9961 cagcattatc agaaggagcc accccacaag atttccagta aaaacagtac atacagacaa
    10021 tggcagcaat ttcaccagta ctacagttaa ggccgcctgt tggtgggcgg ggatcaagca
    10081 ggaatttggc attccctaca atccccaaag tcaaggagta atagaatcta tgaataaaga
    10141 attaaagaaa attataggac aggtaagaga tcaggctgaa catcttaaga cagcagtaca
    10201 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
    10261 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    10321 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggaaagg
    10381 accagcaaag ctcctctgga aaggtgaagg ggcagtagta atacaagata atagtgacat
    10441 aaaagtagtg ccaagaagaa aagcaaagat catcagggat tatggaaaac agatggcagg
    10501 tgatgattgt gtggcaagta gacaggatga ggattaacac atggaaaaga ttagtaaaac
    10561 accatatggg attggaagcc ataataagaa ttctgcaaca actgctgttt atccatttca
    10621 gaattgggtg tcgacatagc agaataggcg ttactcgaca gaggagagca agaaatggag
    10681 ccagtagatc ctagactaga gccctggaag catccaggaa gtcagcctaa aactgcttgt
    10741 accaattgct attgtaaaaa gtgttgcttt cattgccaag tttgtttcat gacaaaagcc
    10801 ttaggcatct cctatggcag gaagaagcgg agacagcgac gaagagctca tcagaacagt
    10861 cagactcatc aagcttctct atcaaagcag taagtagtac atgggcgcgc ccatgtggca
    10921 ggaagtagga aaagcaatgt atgcccctcc catcagtgga caaattagat gttcatcaaa
    10981 tattactggg ctgctattaa caagagatgg tggtaataac aacaatgggt ccgagatctt
    11041 cagacctgga ggaggcgata tgagggacaa ttggagaagt gaattatata aatataaagt
    11101 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
    11161 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
    11221 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctgatat
    11281 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
    11341 cacagtctgg ggcatcaaac agctccaggc aagaatcctg gctgtggaaa gatacctaaa
    11401 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
    11461 gccttggaat gctagttgga gtaataaatc tctggaa
//
