LOCUS       sequence-27422-e         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Wollert T, Wunder C, Lippincott-Schwartz J, Hurley JH
  TITLE     Membrane scission by the ESCRT-III complex.
  JOURNAL   Nature. 2009 Mar 12. 458(7235):172-7.
  PUBMED    19234443
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Mar 12. 458(7235):172-7."
COMMENT     Sequence Label: pMBP-HIS2-Vps20_deltaC
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatataca tatgtcgtac taccatcacc
       61 atcaccatca cgattacatg atcgaagaag gtaaactggt aatctggatt aacggcgata
      121 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
      181 tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg
      241 atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc
      301 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg
      361 atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc
      421 tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
      481 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
      541 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
      601 agtacgacat taaagacgtg g
//
