LOCUS       sequence-274234-       11352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression, Lentiviral. Expression of human 
            phosphofructokinase platelet isoform PFKP-GFP fusion protein with 
            K281R mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen 
            CS, Gao B, Minna JD, Bachoo R, DeBerardinis RJ, Danuser G
  TITLE     Mechanical regulation of glycolysis via cytoskeleton architecture.
  JOURNAL   Nature. 2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 
            10.1038/s41586-020-1998-1.
  PUBMED    32051585
REFERENCE   2  (bases 1 to 11352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Feb 12. pii: 10.1038/s41586-020-1998-1. doi: 
            10.1038/s41586-020-1998-1."
COMMENT     Sequence Label: pLVX neo PFKP-GFP K281R
FEATURES             Location/Qualifiers
     source          1..11352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2685..2687,2688..2690,2691..3404)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2730..2751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2991..3010)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3338..3359
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3422..3996,3997..3999,4000..4008)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3589..3606)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3816..3835
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4009..4803
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4063..4082)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4673..4692
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4817..5405
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4870..4890)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5288..5299)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5612..6245
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6373..6389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6373..6389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6386..6408)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6397..6413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6421..6427,6428..6445,6446..6451))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6466..6487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6604..6621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6775..7363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6855..6874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7534..8325,8326..8394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8157..8176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8395..8499)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8547..8681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8584..8603)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8638..8657
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8849..9482
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9529..9654
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10151..10384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10569..10613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10876..10991
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        11049..11352
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attctttcga gatggacgcg gacgactccc gggcccccaa gggctccttg cggaagttcc
      361 tggagcacct ctccggggcc ggcaaggcca tcggcgtgct gaccagcggc ggggatgctc
      421 aaggtatgaa cgctgccgtc cgtgccgtgg tgcgcatggg tatctacgtg ggggccaagg
      481 tgtacttcat ctacgagggc taccagggca tggtggacgg aggctcaaac atcgcagagg
      541 ccgactggga gagtgtctcc agcatcctgc aagtgggcgg gacgatcatt ggcagtgcgc
      601 ggtgccaggc cttccgcacg cgggaaggcc gcctgaaggc tgcttgcaac ctgctgcagc
      661 gcggcatcac caacctgtgt gtgatcggcg gggacgggag cctcaccggg gccaacctct
      721 tccggaagga gtggagtggg ctgctggagg agctggccag gaacggccag atcgataagg
      781 aggccgtgca gaagtacgcc tacctcaacg tggtgggcat ggtgggctcc atcgacaatg
      841 atttctgcgg caccgacatg accatcggca cggactccgc cctgcacagg atcatcgagg
      901 tcgtcgacgc catcatgacc acggcccaga gccaccagag gaccttcgtt ctggaggtga
      961 tgggacgaca ctgtgggtac ctggccctgg tgagtgcctt ggcctgcggt gcggactggg
     1021 tgttccttcc agaatctcca ccagaggaag gctgggagga gcagatgtgt gtcaaactct
     1081 cggagaaccg tgcccggaaa aaaaggctga atattattat tgtggctgaa ggagcaattg
     1141 atacccaaaa tcgacccatc acctctgaga aaatcaaaga gcttgtcgtc acgcagctgg
     1201 gctatgacac acgtgtgacc atcctcgggc acgtgcagag aggagggacc ccttcggcat
     1261 tcgacaggat cttggccagc cgcatgggag tggaggcagt catcgccttg ctagaggcca
     1321 ccccggacac cccagcttgc gtcgtgtcac tgaacgggaa ccacgccgtg cgcctgccgc
     1381 tgatggagtg cgtgcagatg actcaggatg tgcagaaggc gatggacgag aggagatttc
     1441 aagatgcggt tcgactccga gggaggagct ttgcgggcaa cctgaacacc tacaagcgac
     1501 ttgccatcaa gctgccggat gatcagatcc caaagaccaa ttgcaacgta gctgtcatca
     1561 acgtgggggc acccgcggct gggatgaacg cggccgtacg ctcagctgtg cgcgtgggca
     1621 ttgccgacgg ccacaggatg ctcgccatct atgatggctt tgacggcttc gccaagggcc
     1681 agatcaaaga aatcggctgg acagatgtcg ggggctggac cggccaagga ggctccattc
     1741 ttgggacaaa acgcgttctc ccggggaagt acttggaaga gatcgccaca cagatgcgca
     1801 cgcacagcat caacgcgctg ctgatcatcg gtggattcga ggcctacctg ggactcctgg
     1861 agctgtcagc cgcccgggag aagcacgagg agttctgtgt ccccatggtc atggttcccg
     1921 ctactgtgtc caacaatgtg ccgggttccg atttcagcat cggggcagac accgccctga
     1981 acactatcac cgacacctgc gaccgcatca agcagtccgc cagcggaacc aagcggcgcg
     2041 tgttcatcat cgagaccatg ggcggctact gtggctacct ggccaacatg ggggggctcg
     2101 cggccggagc tgatgccgca tacattttcg aagagccctt cgacatcagg gatctgcagt
     2161 ccaacgtgga gcacctgacg gagaaaatga agaccaccat ccagagaggc cttgtgctca
     2221 gaaatgagag ctgcagtgaa aactacacca ccgacttcat ttaccagctg tattcagaag
     2281 agggcaaagg cgtgtttgac tgcaggaaga acgtgctggg tcacatgcag cagggtgggg
     2341 caccctctcc atttgataga aactttggaa ccaaaatctc tgccagagct atggagtgga
     2401 tcactgcaaa actcaaggag gcccggggca gaggaaaaaa atttaccacc gatgattcca
     2461 tttgtgtgct gggaataagc aaaagaaacg ttatttttca acctgtggca gagctgaaga
     2521 agcaaacgga ttttgagcac aggattccca aagaacagtg gtggctcaag ctacggcccc
     2581 tcatgaaaat cctggccaag tacaaggcca gctatgacgt gtcggactca ggccagctgg
     2641 aacatgtgca gccctggagt gtcccggatc caccggtcgc caccatggtg agcaagggcg
     2701 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     2761 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2821 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     2881 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2941 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3001 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     3061 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     3121 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     3181 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     3241 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     3301 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     3361 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcggatccc
     3421 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     3481 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     3541 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     3601 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     3661 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     3721 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     3781 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     3841 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     3901 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     3961 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccat ggctgaacaa
     4021 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4081 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4141 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     4201 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4261 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4321 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4381 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4441 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4501 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     4561 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4621 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4681 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4741 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4801 tgaacgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4861 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4921 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4981 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5041 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5101 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5161 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     5221 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5281 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5341 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5401 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     5461 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     5521 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     5581 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
     5641 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     5701 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     5761 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     5821 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     5881 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     5941 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     6001 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     6061 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6241 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6301 atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt
     6361 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6421 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6481 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6541 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6601 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6661 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6781 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6841 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6901 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6961 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7021 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7081 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7141 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7201 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7261 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7321 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7381 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7441 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8521 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
     8581 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8641 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     8701 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     8761 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     8821 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     8881 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     8941 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     9001 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     9061 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     9121 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     9181 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     9241 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     9301 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9361 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9421 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9481 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     9541 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     9601 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     9661 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     9721 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     9781 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     9841 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     9901 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     9961 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
    10021 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    10081 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    10141 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    10201 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
    10261 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
    10321 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
    10381 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
    10441 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
    10501 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
    10561 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
    10621 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
    10681 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
    10741 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
    10801 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
    10861 ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10921 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10981 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg ataagcttgg
    11041 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
    11101 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
    11161 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
    11221 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
    11281 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
    11341 gctattacca tg
//
